Gene Information

Name : fliI (Psyr_3454)
Accession : YP_236524.1
Strain : Pseudomonas syringae B728a
Genome accession: NC_007005
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 4116887 - 4118245 bp
Length : 1359 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGCGCCTTGATCGCACCAGTTTCGCCAAGCGCCTCGGTTCCTATGCCGAGTCGATCAGCCTGCCTGCCCAGCCCGTAGT
GGAAGGTCGGCTGCTGCGCATGGTGGGTCTGACGCTTGAGGCCGAGGGCCTGCGCGCGGCAATGGGCAGCCGGTGTGTGG
TTATCAATGACGACAGCCATCACCCGGTGGAAGTCGAAGCTGAAGTGATGGGTTTTTCCGGTGGCAAGGTGTTTCTCATG
CCGGTCGGCAGCGTGGCCGGCATCGCGCCTGGCGCGCGGGTTGTGCCGCTGGCCGATACCGGTCGTCTGCCGATGGGCAT
GAGCATGCTGGGCCGGGTGCTTGATGGTGCCGGTCGTCCGCTGGATGGTCGCTCGCCGATCAAGGCTGAAGACTGGGTGC
CGATGGATGGCCCGACCATCAACCCGCTCAAGCGTGACCCCATCAGTCAGCCACTGGATGTCGGCATTCGGTGCATCAAC
GGTTTATTGACGGTGGGTCGCGGTCAGCGTCTCGGGCTGTTTGCCGGTACGGGCGTGGGCAAGAGTGTGCTGCTCGGCAT
GATGACGCGCTTCACCGAAGCGGACATCATCGTCGTCGGCCTGATCGGGGAGCGGGGCCGTGAGGTCAAGGAGTTCATCG
AGCACATCCTCGGCGAGGAGGGCCTGAAGCGTTCGGTGGTGGTCGCTTCGCCAGCCGATGATGCGCCTCTGATGCGTTTG
CGCGCAGCGATGTATTGCACGCGAATCGCCGAATACTTTCGTGACAAGGGCAAGAATGTCCTGTTGCTGATGGATTCCCT
GACCCGTTTTGCTCAGGCTCAGCGGGAAATCGCGCTGGCCATTGGCGAGCCGCCGGCCACCAAGGGCTATCCTCCATCAG
TGTTTGCCCGCTTGCCCAAACTGGTCGAGCGCGCCGGCAACGCTGAAAAGGGCGGTGGTTCTATTACCGCGTTTTACACC
GTGCTCTCCGAAGGCGATGACCAGCAGGACCCGATTGCCGACTCGGCGCGCGGTGTGCTGGACGGTCACATCGTGTTGTC
CCGGCGTCTGGCCGAAGAGGGGCATTACCCGGCTATCGATATCGAAGCGTCGATCAGCCGGGTCATGCCCGCCGTGGTCA
GCCCCGAACACATGGCGCGTGCGCAGCATTTCAAACAGTTGTGGTCGCGTTATCAGCAGACGCGCGATCTGATCAGCGTC
GGCGCCTACGTGGCCGGTGGTGATCGCGAGACGGACATGGCGATCGCGCTGCATCCGGTACTGGTGCGCTATCAGCGTCA
GGCGTTGCGCGAGAACGAAAGCATGCAGGGCAGCAGCGAGGCGCTGGCGTCGATCTTCGCACCAGCGCCTGGCGGCTGA

Protein sequence :
MRLDRTSFAKRLGSYAESISLPAQPVVEGRLLRMVGLTLEAEGLRAAMGSRCVVINDDSHHPVEVEAEVMGFSGGKVFLM
PVGSVAGIAPGARVVPLADTGRLPMGMSMLGRVLDGAGRPLDGRSPIKAEDWVPMDGPTINPLKRDPISQPLDVGIRCIN
GLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTEADIIVVGLIGERGREVKEFIEHILGEEGLKRSVVVASPADDAPLMRL
RAAMYCTRIAEYFRDKGKNVLLLMDSLTRFAQAQREIALAIGEPPATKGYPPSVFARLPKLVERAGNAEKGGGSITAFYT
VLSEGDDQQDPIADSARGVLDGHIVLSRRLAEEGHYPAIDIEASISRVMPAVVSPEHMARAQHFKQLWSRYQQTRDLISV
GAYVAGGDRETDMAIALHPVLVRYQRQALRENESMQGSSEALASIFAPAPGG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-79 51
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 8e-79 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-78 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-78 50
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-78 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-87 50
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-87 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-89 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-85 50
lscN AAO18044.1 LscN Virulence TTSS locus Protein 7e-84 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 6e-88 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-88 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-89 48
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 4e-75 48
unnamed AAL06369.1 EscN Virulence LEE Protein 4e-75 48
escN AAC31513.1 L0034 Virulence LEE Protein 2e-77 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-77 48
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-77 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-77 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-76 48
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-76 48
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-76 48
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-76 48
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-77 48
escN AAC38383.1 EscN Virulence LEE Protein 1e-77 48
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-72 48
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 4e-72 48
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 6e-69 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-89 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 4e-89 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 4e-89 47
escN AAK26715.1 EscN Virulence LEE Protein 7e-76 47
escN AAL57542.1 EscN Virulence LEE Protein 7e-76 47
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-72 46
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 5e-77 46
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 8e-72 46
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 8e-72 46
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-72 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-71 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-71 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-72 44
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-68 43
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-68 43
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-68 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-62 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 1e-62 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-62 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-62 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 9e-66 41
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 1e-64 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_236524.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 85
fliI YP_236524.1 flagellum-specific ATP synthase VFG2499 Protein 6e-128 62
fliI YP_236524.1 flagellum-specific ATP synthase VFG2331 Protein 8e-125 61
fliI YP_236524.1 flagellum-specific ATP synthase VFG0515 Protein 5e-79 50
fliI YP_236524.1 flagellum-specific ATP synthase VFG2144 Protein 5e-90 48
fliI YP_236524.1 flagellum-specific ATP synthase VFG0048 Protein 6e-89 48
fliI YP_236524.1 flagellum-specific ATP synthase VFG0390 Protein 1e-86 48
fliI YP_236524.1 flagellum-specific ATP synthase VFG0812 Protein 9e-78 48
fliI YP_236524.1 flagellum-specific ATP synthase VFG0730 Protein 6e-78 48
fliI YP_236524.1 flagellum-specific ATP synthase VFG0192 Protein 4e-87 47
fliI YP_236524.1 flagellum-specific ATP synthase VFG0555 Protein 6e-63 42