Gene Information

Name : lysS (Psyr_1311)
Accession : YP_234400.1
Strain : Pseudomonas syringae B728a
Genome accession: NC_007005
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1488289 - 1489791 bp
Length : 1503 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGCGACCAACAACTCGACCCGCAAGCCCTGCAACAGGAAGAAAACACCCTGATCGCCTTGCGCAAGGAAAAGCTTGC
TGCAGGCCGTGCCAAGGGTCAGGCCTTCCCCAATGACTTCCGCCGCGACAGCTACTGCAATGACCTGCAGAAACAGTACG
TCGACAAGACCAAGGAAGAACTGGCAGAGGCAGCGATTCCGGTCAAGGTTGCCGGTCGCATCATGCTCAACCGTGGCTCG
TTCATGGTGATCCAGGACATGACCGGGCGCATCCAGGTCTATGTCAACCGCAAGACCCTGCCCGAAGAAACCCTGGCCGA
GGTGAAAACCTGGGATCTGGGCGACATCATCGCCGCCGAAGGTACCCTGGCACGCTCCGGCAAGGGCGACCTGTACGTCG
AGATGACCAGCGTTCGCCTGCTGACCAAGTCCTTGCGTCCGCTGCCCGACAAGCACCACGGCCTGACCGACACCGAACAG
CGCTACCGTCAGCGTTACGTCGACCTGATCGTCAACGAGGAAGTGCGTGAGACCTTCCGCGTGCGTTCGCAGGTCATCGC
TCACATCCGCAGCTTCCTGATGAAGCGCGACTTCCTCGAAGTCGAAACGCCGATGCTGCAGACCATTCCGGGCGGCGCGG
CGGCCAAGCCGTTCGAAACCCACCACAACGCGCTGGACATGGAAATGTTCCTGCGTATCGCCCCGGAGCTGTACCTCAAG
CGTCTGGTGGTCGGTGGTTTCGAGAAGGTCTTCGAGATCAATCGCAACTTCCGTAACGAAGGTGTTTCGACCCGGCACAA
CCCCGAGTTCACCATGCTCGAGTTCTACCAGGCCTACGCCGATTACGAAGACAACATGGACCTCACCGAGGAACTGTTCC
GCGAACTGGCGCAACTGGTTCTGGGCAGCACCGATGTGCCTTATGGCGACAAGGTGTTCCACTTCGGCGAACCGTTCGTG
CGCCTGTCGGTGTTCGACTCGATCCTCAAGTACAACCCTGAGCTGACCGTTGCCGACCTGCAGGACATCGACAAGGCGCG
CGCCATTGCGAAAAAGGCCGGTGCCAAGGTGCTGGGCTTCGAAGGCCTGGGCAAATTGCAGGTGATGATTTTCGAAGAGC
TGGTCGAGCACAAGCTGGAACAGCCGCACTTCATCACCCAGTACCCGTTTGAAGTGTCGCCGCTGGCGCGTCGCAACGAC
GAAAACCCGAACGTCACCGACCGTTTCGAGCTGTTCATCGGTGGTCGCGAAATCGCCAATGCCTACTCCGAGCTCAACGA
CGCCGAAGATCAGGCCGAACGCTTCCAGGCTCAGGTGGCCGACAAGGATGCCGGTGACGACGAAGCCATGCACTACGACG
CCGACTTCGTTCGTGCGCTGGAGTACGGCATGCCGCCGACGGCAGGCGAGGGGATCGGTATCGATCGGTTGGTGATGTTG
CTCACCGACTCGCCGTCGATTCGCGACGTGATCCTCTTCCCGCACATGCGCCCGCAAGCGTAA

Protein sequence :
MSDQQLDPQALQQEENTLIALRKEKLAAGRAKGQAFPNDFRRDSYCNDLQKQYVDKTKEELAEAAIPVKVAGRIMLNRGS
FMVIQDMTGRIQVYVNRKTLPEETLAEVKTWDLGDIIAAEGTLARSGKGDLYVEMTSVRLLTKSLRPLPDKHHGLTDTEQ
RYRQRYVDLIVNEEVRETFRVRSQVIAHIRSFLMKRDFLEVETPMLQTIPGGAAAKPFETHHNALDMEMFLRIAPELYLK
RLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFV
RLSVFDSILKYNPELTVADLQDIDKARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRND
ENPNVTDRFELFIGGREIANAYSELNDAEDQAERFQAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVML
LTDSPSIRDVILFPHMRPQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-134 60
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 8e-78 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_234400.1 lysyl-tRNA synthetase VFG1668 Protein 3e-134 60