Gene Information

Name : kpyK (LBA0957)
Accession : YP_193840.1
Strain : Lactobacillus acidophilus NCFM
Genome accession: NC_006814
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 933614 - 935383 bp
Length : 1770 bp
Strand : +
Note : COG3848 Phosphohistidine swiveling domain

DNA sequence :
ATGAAGAAAACTAAGATTGTTAGTACTTTAGGACCAGCTTCAAACGATATTGAAACTATTACTGCTTTAGCTAACGCAGG
CGCAAACGTATTCCGTTTCAACTTCTCACACGGTGACCATGAAGAACACCGTTCACGTATGAACATGGTTCGTGAAGTGG
AAAAGAATACTGGTAAGCTTCTTGGTATTGCTCTTGATACTAAGGGTGCTGAAATCAGAACCACTGAACAAGAAGGTGGC
AAGTTTACTATCAATACTGGTGACACAATCCGTATCTCAATGGACGATACTAAGAAGGGTAACAAGGACATGATCCACGT
TACCTACGACGGCTTGTACGATGATACTCACGTTGGTGGTCACGTATTAATCGATGATGGTTTGGTTGACCTTTTGATCA
AGGAAAAGGACGACGCTAAGAGAGAATTAGTTTGTGAAGCTCAAAACACTGGTTTAATCGGTTCAAAGAAGGGTGTTAAC
GCTCCAGGTGTTGAAATTCGCCTCCCAGGTATCACTCAAAAAGATACTGATGACATTAACTTTGGTTTGAAGGAAGGTAT
CAACTTTATCTTTGCTTCATTCGCACGTAAGGCTCAAGATATCTTAGACATCCGTAAGCTTTGTGAAGCAGCAGGTGCTG
ACTACGTAAAGATTTTCCCTAAGATCGAATCACAAGAAGGTATCGACAACGCTGACGAAATTTTACAAGTTTCTGATGGT
TTGATGGTTGCTCGTGGTGACATGGGTGTTGAAATTCCATTTATGGAAGTTCCATTTGTACAAAAGAACTTAATCAGAAA
AGCTAACGCACTTGGTAAGCCAGTTATTACTGCTACTCAAATGCTTGACTCAATGCAAGAAAACCCACGTCCAACTCGTG
CTGAAGTTTCTGACGTTGCTAACGCTGTTCTTGACGGTACTGATGCAACTATGCTTTCAGGTGAATCAGCAAACGGTTTG
TACCCAGTTAACGCTGTTAAAGCTATGGCTGAAATCGATGAACGTACTGAAAAGCAAATGCTTAAGCGTAACACTTTAGC
ACTTCAAAGATTTGAAGCATACAAGGGCTCAAACGTAACTGAAGCTATCGGTGAATCAGTTGTACGTACTGCACAAGAAT
TAGGTGTTAAGACTATCATCGCTGCAACTAACTCAGGTTACACTGCTCGTATGATTTCTAAGTACCGTCCAAATGCTGAC
ATCGTTGCAATGACCTTCGATGAAAAGATCCAACACTCATTGGCAGTTGTTTGGGGTGTACAACCAGTATTAACTGACAA
GCCTAAGTCAACTGATGACATGTTTGAAAAGGCAGCAGAAGTTGCTAAGGAAAAGGGCTACGTTAAGGATGGTGACCTTG
TAATTATCGTTGCTGGTGTTCCAGTTGGTGATTCAGGTACTACTAACTTGATGAAGTTACAAATTATCGGTAACAAGCTT
GTTCAAGGTTTAGGTGTTGGTAACGGTTCAGTTATCGGCAAGGCTGTTGTTGCAAACTCAGCAGAAGAAGCTAACAGCAA
GGTTAAGGAAGGCGACATCTTAGTTGCTAAGACTACTGATCCAGACTACAACCCAGCTATCAAGAAGGCTTCAGGTTTGG
TTGTTGAAGCTTCAGGTTTAACTTCACACGCAGCAGTTATTGGTTTATCATTAGGTATCCCAGTTGTTGTTGGTGCTACT
GACGCTACTGAAAAGATTTCTGACGGTTCAACTATTACTGTTGATGCTCGTCGTGGTGCTATTTACCAAGGTGAAATCTC
AAACCTTTAA

Protein sequence :
MKKTKIVSTLGPASNDIETITALANAGANVFRFNFSHGDHEEHRSRMNMVREVEKNTGKLLGIALDTKGAEIRTTEQEGG
KFTINTGDTIRISMDDTKKGNKDMIHVTYDGLYDDTHVGGHVLIDDGLVDLLIKEKDDAKRELVCEAQNTGLIGSKKGVN
APGVEIRLPGITQKDTDDINFGLKEGINFIFASFARKAQDILDIRKLCEAAGADYVKIFPKIESQEGIDNADEILQVSDG
LMVARGDMGVEIPFMEVPFVQKNLIRKANALGKPVITATQMLDSMQENPRPTRAEVSDVANAVLDGTDATMLSGESANGL
YPVNAVKAMAEIDERTEKQMLKRNTLALQRFEAYKGSNVTEAIGESVVRTAQELGVKTIIAATNSGYTARMISKYRPNAD
IVAMTFDEKIQHSLAVVWGVQPVLTDKPKSTDDMFEKAAEVAKEKGYVKDGDLVIIVAGVPVGDSGTTNLMKLQIIGNKL
VQGLGVGNGSVIGKAVVANSAEEANSKVKEGDILVAKTTDPDYNPAIKKASGLVVEASGLTSHAAVIGLSLGIPVVVGAT
DATEKISDGSTITVDARRGAIYQGEISNL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-79 47
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 8e-80 47
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 8e-80 47
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-79 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
kpyK YP_193840.1 pyruvate kinase VFG0479 Protein 3e-80 47