Gene Information

Name : pykA (ABC2718)
Accession : YP_176214.1
Strain : Bacillus clausii KSM-K16
Genome accession: NC_006582
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2851814 - 2853568 bp
Length : 1755 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGCGTAAAACAAAAATTGTTTGTACGATTGGACCAGCAAGTGAGTCGCTTGAAAAACTAACAGCACTCATCGAAGCGGG
GATGAATGTAGCCCGTTTGAATTTTTCACACGGTGATTTTGAAGAGCACGGTGCCCGAATTAAAAACATTCGCGAAGCGG
CAAAACGGACTGGAAAAACGGTTGCTATTCTACTTGATACGAAAGGGCCGGAAATCCGCACACAAACGGTAGAAGGCGGA
GCGATTGAACTTGAGAAAGGGCAAGAGCTCATTTTGTCAATGAATGAAGTCCAAGGTACGAAAGAAAAGATCTCCATTAC
GTATCCAGGGCTTTGCGAGGATGTAGAAGTCGGAAGTACGATCTTGCTCGATGATGGCTTAATTGGGCTTGAGGTAAAGG
AAATCCGCGACACTGAGCTGGTTACGATTGTTCGCAACAGTGGCGTGCTGAAAAACAAGAAAGGCGTCAACGTCCCAAAT
GTCAGCGTCAACCTACCGGGGATGACCGAAAAGGATGCAGCCGACATTGTATTTGGGATTGAGCAAAAAGTTGACTTTAT
AGCCGCCTCCTTTGTGCGCCGCGCTTCTGATGTTCTAGAAATCCGCGAGCTCCTTGAAAAGCATGGCGCTGCAGATACAG
CGATTATCCCGAAAATCGAAAACCAAGAAGGCGTTGACAATATCGATGAAATTCTTGAAGTATCGGACGGCCTTATGGTC
GCAAGGGGAGACTTAGGTGTAGAAATTCCAGCAGAAGAAGTACCGCTTGTGCAAAAACAGCTCATTAAAAAATGCAATGA
GTTGGCCAAGCCGGTCATCACTGCCACGCAAATGCTTGATTCCATGCAACGCAACCCGCGTCCTACACGAGCGGAAGCAA
GCGACGTAGCCAACGCTATTTTTGACGGGACAGACGCCATTATGCTTTCTGGTGAAACCGCTGCTGGTGATTACCCTGTA
GAATCGGTGCAAACAATGCACAATATTGCCGAACGAACAGAACAAGCCCTCAATTATGAAAACATGCTGCGTCGGAAAAG
CAAAGAAGTGAAAACATCGATTACAAGTGCAATTGGCCAGTCTGTTGCTTACACCGCCTCAAACTTGAATGCTGCTGCGA
TTTTAACAGCGACAGAAAGCGGCTATACAGCACGGATGACATCACGTTTCCGGCCGAAATCGCCAATCTTGGCCGTAACG
AGCAATGAGCGTGCTTACCGGAGAATGGCGCTCGTGTGGGGGGTTACGCCAATACTTGGTAAACAAGCAAACACAACTGA
CGAAATGTTAGACCGTACAGTTGAAGCGGCTGTCGCTACAGGAGTGGTGAAACAAGGTGACCTTGTTATCATTACGGCTG
GTGTTCCCGTTGGGGAGTCAGGCACAACGAATTTAATGAAAGTCCATATGCTTGGGGAAGTTGCAGCAAAAGGACAAGGC
ATCGGACGTCGCTCTGCCTCAGGAAAAGTCGTTGTTGCCCGTGACGCAAAAGAAGCGATTGCAAAAGTAACGGAAGGCTC
TGTGCTTGTCACAAAATCAACCGATAAAGACATGATCGAAGCGTTCGAGAAAGCTGCTGCTGTCGTGACTGTAGAAGGCG
GTCTTACATCCCATGCAGCTGTTGTTGGCTTAAACTTGAGCACGCCTGTTATCGTCGGTGTAGCCAACGCCCTTGAACGT
TTCCAAGACGGCGATGAAGTGACTGTGGACTGCATTAGCGGAAACATTTACAGCGGCCAAGCCCGTGTATTATAA

Protein sequence :
MRKTKIVCTIGPASESLEKLTALIEAGMNVARLNFSHGDFEEHGARIKNIREAAKRTGKTVAILLDTKGPEIRTQTVEGG
AIELEKGQELILSMNEVQGTKEKISITYPGLCEDVEVGSTILLDDGLIGLEVKEIRDTELVTIVRNSGVLKNKKGVNVPN
VSVNLPGMTEKDAADIVFGIEQKVDFIAASFVRRASDVLEIRELLEKHGAADTAIIPKIENQEGVDNIDEILEVSDGLMV
ARGDLGVEIPAEEVPLVQKQLIKKCNELAKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGDYPV
ESVQTMHNIAERTEQALNYENMLRRKSKEVKTSITSAIGQSVAYTASNLNAAAILTATESGYTARMTSRFRPKSPILAVT
SNERAYRRMALVWGVTPILGKQANTTDEMLDRTVEAAVATGVVKQGDLVIITAGVPVGESGTTNLMKVHMLGEVAAKGQG
IGRRSASGKVVVARDAKEAIAKVTEGSVLVTKSTDKDMIEAFEKAAAVVTVEGGLTSHAAVVGLNLSTPVIVGVANALER
FQDGDEVTVDCISGNIYSGQARVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-108 53
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-108 53
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-108 53
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-108 53
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 4e-65 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_176214.1 pyruvate kinase VFG0479 Protein 5e-109 53