Gene Information

Name : fliI (ABC2266)
Accession : YP_175762.1
Strain : Bacillus clausii KSM-K16
Genome accession: NC_006582
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 2390592 - 2391905 bp
Length : 1314 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
TTGCAAAAGCTCATTGATCATGTGCACACACTTTCTCCCTATAAGTGGTATGGACAAGTTCGCCATGCTGCGGGGTTGAC
GATTGAATCAGCCGGGCCGAGGGCATTTATCGGCGAACTGTGCTATATCTGGGCTAGCCCAGATCAAAAGGGGACTAAAG
TTAGAGCTGAAGTCGTTGGCTTTAAGGATGACCGCATTTTGCTCATGCCGCTAGACGATACGCGGCGAATAGCCCCTGGG
AGCTATGTAGAAGGAACAGGAAAACCGTTAAATGTAGCGTGTGGACCAAGTTTGATTGGCCAAATTGTCAATGGCGTCGG
TGAACCATTTTTGCAAAGCGCAGCATTGGGGGATACTGACGCCAACTATTCGACGGACAATTCACCTCCAAACCCGCTAG
AAAGACCGCGCATTTCCGAAATTATGAGTGTTGGTGTCCGCGCAATTGACGGGCTGTTGACAATGGGAAAAGGGCAAAGA
GTTGGCATTTTTGCTGGCAGCGGTGTAGGGAAGAGCACACTCATGTCGATGATTGCTAAACAATCAGATGCAGATGTGAA
TGTGATTGCTTTAATTGGTGAACGGGGCAGAGAAGTAAAAGAATTTGTTGAGCGCGACTTAGGAGAGGAAGGGCTTAAAA
AGTCTGTTGTTGTCGTCGCAACATCAGACCAGCCAGCACTGTTACGAGTGAAAGGAGCGCAAACAGCCACTGCCATTGCT
GAATACTTCCGCGACAAGGGCAAGAATGTCAATTTAATGATGGATTCGGTCACCCGGTTTGCAATGGCACAGCGCGAAAT
TGGCTTGGCAATTGGTGAACCACCGACGACAAAAGGCTACCCCCCTTCTGTTTTTGCGATGATGCCAAAGCTGCTTGAAC
GGTCAGGTACAAACCAGTCAGGCTCGATCACCGGTTTATACACCGTTCTTGTAGACGGCGACGACATGAATGAGCCTATT
GCAGATGCCGTGCGAGGAATTCTTGACGGACACTTTGTATTGGACAGGCAACTCGCCAATCGTGGGCAGTATCCTGCCGT
TAATGTGTTAAAAAGCGTTAGCAGGCTAATGAAAGACTTGGTCGAAAACGATCATCTTCATGCAGCAAAGCTTTTTAGGC
AACGTTTAGCCGATTACGAGCAGTCAGAAGACTTAATTTCACTCGGTGCTTATAAACAGGGCAGTTCCAAAGACATTGAC
GCTGCAATTCATGACCATCCGGCTATGCTTGACTTTCTTAAACAAGAAGTCGAAGAAAGCGATTCGTTTGCAGAAACGAA
AACAAAGCTTATACAACTTTTTAAACAGGAGTGA

Protein sequence :
MQKLIDHVHTLSPYKWYGQVRHAAGLTIESAGPRAFIGELCYIWASPDQKGTKVRAEVVGFKDDRILLMPLDDTRRIAPG
SYVEGTGKPLNVACGPSLIGQIVNGVGEPFLQSAALGDTDANYSTDNSPPNPLERPRISEIMSVGVRAIDGLLTMGKGQR
VGIFAGSGVGKSTLMSMIAKQSDADVNVIALIGERGREVKEFVERDLGEEGLKKSVVVVATSDQPALLRVKGAQTATAIA
EYFRDKGKNVNLMMDSVTRFAMAQREIGLAIGEPPTTKGYPPSVFAMMPKLLERSGTNQSGSITGLYTVLVDGDDMNEPI
ADAVRGILDGHFVLDRQLANRGQYPAVNVLKSVSRLMKDLVENDHLHAAKLFRQRLADYEQSEDLISLGAYKQGSSKDID
AAIHDHPAMLDFLKQEVEESDSFAETKTKLIQLFKQE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-97 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-92 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 5e-83 50
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-93 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-93 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 7e-93 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-92 49
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 9e-93 49
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 9e-93 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-88 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-88 48
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-77 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-83 46
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-83 46
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-84 46
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-84 46
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-84 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-79 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-76 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-76 44
escN AAC38383.1 EscN Virulence LEE Protein 4e-79 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 7e-81 44
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-80 44
escN AAK26715.1 EscN Virulence LEE Protein 2e-79 44
escN AAL57542.1 EscN Virulence LEE Protein 2e-79 44
escN AAC31513.1 L0034 Virulence LEE Protein 1e-79 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-79 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-79 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-80 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-79 44
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-80 44
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-79 44
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-72 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 3e-75 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-75 44
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-79 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-83 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-78 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-78 44
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 7e-74 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-74 43
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-70 43
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-70 43
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-70 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-70 43
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 4e-74 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 6e-72 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 6e-72 41
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 5e-71 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_175762.1 flagellum-specific ATP synthase VFG2144 Protein 1e-100 50
fliI YP_175762.1 flagellum-specific ATP synthase VFG0192 Protein 2e-97 50
fliI YP_175762.1 flagellum-specific ATP synthase VFG0390 Protein 1e-96 50
fliI YP_175762.1 flagellum-specific ATP synthase VFG1254 Protein 6e-97 50
fliI YP_175762.1 flagellum-specific ATP synthase VFG0048 Protein 4e-94 49
fliI YP_175762.1 flagellum-specific ATP synthase VFG2499 Protein 1e-91 47
fliI YP_175762.1 flagellum-specific ATP synthase VFG2331 Protein 2e-93 46
fliI YP_175762.1 flagellum-specific ATP synthase VFG0515 Protein 2e-83 46
fliI YP_175762.1 flagellum-specific ATP synthase VFG0730 Protein 2e-79 44
fliI YP_175762.1 flagellum-specific ATP synthase VFG0812 Protein 8e-80 44
fliI YP_175762.1 flagellum-specific ATP synthase VFG2459 Protein 3e-82 43
fliI YP_175762.1 flagellum-specific ATP synthase VFG0555 Protein 1e-70 43
fliI YP_175762.1 flagellum-specific ATP synthase VFG1008 Protein 2e-70 43
fliI YP_175762.1 flagellum-specific ATP synthase VFG1778 Protein 1e-70 41