Gene Information

Name : lysS (ABC0117)
Accession : YP_173621.1
Strain : Bacillus clausii KSM-K16
Genome accession: NC_006582
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 134564 - 136072 bp
Length : 1509 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
TTGAGAGCGATGACGGAAGGATTGGAAATGCATGATTTGCTTGCCGTGCGCCGAGACAAGTTAAATCAATTGTATGATGA
AGGGATTAACCCATTTGGCGGAAAGTTTGAACGGACGCATACATCAAATGAAGTGATTGAGCAATTTGCTGCGTTTACGA
AAGAAGAGCTTGAAGAAAAAGACGATCATGTCGTCCTTGCAGGGCGATTAATGACAAAACGCGGCAAAGGAAAGGCCGGG
TTTGCCCACATTCAAGATATAGGAGGGCAGATTCAAATATACGTTCGTCAGGATGCCGTAGGGGAAGAAGCGTATGGTTT
ATTCAAATCATTAGATATTGGCGACATGGTTGGTGTCGGCGGGCGTCCATTTAAGACGAAGGTAGGAGAAATGTCTGTGA
AAGTGACGGAGCTGGTGATACTAGGGAAATCATTGCGTCCGTTGCCAGACAAATATCACGGTTTAAAAGACGTTGAGCAA
CGTTACCGCCAGCGTTATCTTGATCTAATTACGAGTCCTGAATCAAGAACCGCGTTTATAACAAGAAGTAAAATCATTCA
AGTCATGCGCCGTTATTTAGATGAAAAAGGCTTTCTTGAAGTGGAAACGCCGATGATGCATAGCATCCCTGGTGGAGCTT
CTGCTAGGCCATTTGTCACTCACCATAATACGCTCGATATGGAATTATACATGCGTATTGCGATTGAGCTTCATTTAAAG
CGTTTGATCGTTGGCGGCTTGGAAAAAGTATACGAAATAGGCCGTGTGTTCCGAAATGAAGGGATATCAACTCGCCATAA
CCCAGAATTTACAATGATTGAACTTTATGAAGCGTATGCTGATTACCATGACATTATGGAACTGACAGAAGGTGTAATCG
CGCACATTGCTAAGGAAGTCTTAGGCACGACCACGGTCCAGTACGGAGAACACACCGTTGAACTGGCACCTAGTTGGAAG
CGAGTCCATATGGTTGATGCGATTAAAGAAAAGACTGGCGTCGATTTTTGGCAGCAGATGAGCGATGGAGAAGCGCGGGC
ACTTGCAAAAGAACATGGAGTGCCAGTAAAAGAAACAATGACATTTGGCCATGTTGTAAACGAGTTTTTTGAGCACTTTA
TAGAAGAAACGCTTATTCAGCCTACATTTGTATATGGCCATCCTTTGGCGATCTCCCCGCTTGCAAAGAAAAATGAGGAA
GACCCGCGCTTTACTGATCGCTTTGAGCTGTTTATTGTTGGGCGTGAACATGCCAATGCATTCTCTGAACTTAATGATCC
GATTGACCAGAGAAAACGCTTTGAAGCACAATTAATTGAACGTGAGCAAGGTGACGATGAGGCCCACCGAATGGATGAAG
ATTTTATTGAAGCACTTGAATACGGCATGCCTCCAACAGGCGGTCTCGGTATTGGCATTGATAGACTTGTGATGTTGTTG
ACAAATTCACCTTCCATCCGGGACGTCATTCTCTTCCCGCAAATGCGTAACCGCGAGCAAGGCGAATAA

Protein sequence :
MRAMTEGLEMHDLLAVRRDKLNQLYDEGINPFGGKFERTHTSNEVIEQFAAFTKEELEEKDDHVVLAGRLMTKRGKGKAG
FAHIQDIGGQIQIYVRQDAVGEEAYGLFKSLDIGDMVGVGGRPFKTKVGEMSVKVTELVILGKSLRPLPDKYHGLKDVEQ
RYRQRYLDLITSPESRTAFITRSKIIQVMRRYLDEKGFLEVETPMMHSIPGGASARPFVTHHNTLDMELYMRIAIELHLK
RLIVGGLEKVYEIGRVFRNEGISTRHNPEFTMIELYEAYADYHDIMELTEGVIAHIAKEVLGTTTVQYGEHTVELAPSWK
RVHMVDAIKEKTGVDFWQQMSDGEARALAKEHGVPVKETMTFGHVVNEFFEHFIEETLIQPTFVYGHPLAISPLAKKNEE
DPRFTDRFELFIVGREHANAFSELNDPIDQRKRFEAQLIEREQGDDEAHRMDEDFIEALEYGMPPTGGLGIGIDRLVMLL
TNSPSIRDVILFPQMRNREQGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-122 52

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_173621.1 lysyl-tRNA synthetase VFG1668 Protein 7e-123 52