Gene Information

Name : lysS (mhp174)
Accession : YP_115687.1
Strain : Mycoplasma hyopneumoniae 232
Genome accession: NC_006360
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 211544 - 213214 bp
Length : 1671 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGATTCTTAATCAAATTCTTAAATCCCAACCAGAAACTAGTCCAGAAACAGGAACAGATTCAAGTCAAGAAAATTTC
TAATTACAAAAAAACTCTATTTTTACTAACTTTTATAAATTATTTTTGAAATTTGCAAAAGTTAGTAAAAATTAAAAAGT
TTTTTCAAAAGAAGCTTTTTAATTTTATTAAGGTGATGAAAATTATGTCAAAACTAAATGATCAACAACAATTTCGCCGC
GATAAGCTTAAAAATTTAGTTAAAAATGGTTTTAATTTCCCTAGTTCAACTTTTGAGCATGATAATTTAGTAGAAATAAA
TGAAAAATTTAGTCAAAAATCGAAGCAATTTTTCCTTGAAAATCAAGTAAAAATTGCTTTTGCTGGTCGACTTATTCGCC
AACGCGGCCCATTTTTTATAATTTTTAGTCAAAACTTGCAATTTCAAGCTTATATTTCAAAAGAATTTCAAAAAAAAAAT
GAATTTATCTTTGCAAATTTAGATCTTGGTGATATAATTGAGGTTTCAGGTTATCTTTTCAAGACGCAAACTGGTCAACT
TAGTATTAAAGTTAATAATTTTTCTCTGCTTACAAAGTCCTTACACCCTTTACCAGATCAGTATTATGGTATTGAAAATC
CAGATGAGAAATACAGGAAACGTTATCTTGACCTGCTTGTAAATTCAGAATCTGCAAAAACTTTTCGACTTAGAAGTAAA
ATTATCTCCTTAATCCGTACATTTTTTGATTCCCAAGGTTTTTTAGAAGTTGATACTCCCGTTTTACAGCCAGTTTTAGG
CGGTGCATCAGCAAAACCTTTTATTACTTATTATAATTCTCTTAGTCAAAATTTTTATCTCCGAATTGCCACGGAATTAC
CGCTGAAAAAATTATTAGTTGCCGGTTTTGATCGTGTCTATGAAATTGGTAAAATTTTTCGTAATGAGGGTTTTGATTCA
ACTCATAATCCTGAATTCACCTCAATTGAATTTTATCAGGCTTATGCAAATTTAGAGAAAATAATGGATCAAACCGAAAA
TTTATTTCGTTTTTTATTTGAAAAATTAAATCTTGATCCGGCAAATTTTGATTTTTCAAATAAAAAAATTAATTTCTTGG
AAAAATTTGCCCGTTATGATATGATTGAGATTACTTCAAAATTAATGAACTTCGATTTAAAAAGTGCTAATTTTGCTGAT
TTAGTCGAAAAAGCAAAAAAAGAAGGGGTGAAGATCGAGCCATTTTTCAAAAAAGGGCATCTAATCAACAAATTTTTTGA
AAAATTTGTTGAACCAACACTAATAAATCCAACTTTTATCATAGGTCATCCAATTGAAATTTCCCCGCTAGCCAAGTCAA
ATCCAAACAATCCTAATTTTACTCTCAGGGCCGAATTATTTATCTGTGGTAAAGAATTTGCGAATATGTTTGATGAATTA
AATGATCCAATTGACCAATTAAGTCGTTTTCAAGCCCAAATTATCGAAAAAAATCAGGGAAATCAAGAAGCAAGTGAGAT
CGACAATGAATTTGTTCAAGCACTTGAATATGGAATGCCACCCGCCGGGGGTTGTGGCATTGGCATTGACAGATTGACAA
TGCTTTTAACAAAGAATGAATCAATCCGTGAGGTAATTTTATTCCCACAGCTAAAACCAAAAAAGGACTAA

Protein sequence :
MRFLIKFLNPNQKLVQKQEQIQVKKISNYKKTLFLLTFINYFWNLQKLVKIKKFFQKKLFNFIKVMKIMSKLNDQQQFRR
DKLKNLVKNGFNFPSSTFEHDNLVEINEKFSQKSKQFFLENQVKIAFAGRLIRQRGPFFIIFSQNLQFQAYISKEFQKKN
EFIFANLDLGDIIEVSGYLFKTQTGQLSIKVNNFSLLTKSLHPLPDQYYGIENPDEKYRKRYLDLLVNSESAKTFRLRSK
IISLIRTFFDSQGFLEVDTPVLQPVLGGASAKPFITYYNSLSQNFYLRIATELPLKKLLVAGFDRVYEIGKIFRNEGFDS
THNPEFTSIEFYQAYANLEKIMDQTENLFRFLFEKLNLDPANFDFSNKKINFLEKFARYDMIEITSKLMNFDLKSANFAD
LVEKAKKEGVKIEPFFKKGHLINKFFEKFVEPTLINPTFIIGHPIEISPLAKSNPNNPNFTLRAELFICGKEFANMFDEL
NDPIDQLSRFQAQIIEKNQGNQEASEIDNEFVQALEYGMPPAGGCGIGIDRLTMLLTKNESIREVILFPQLKPKKD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 4e-85 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_115687.1 lysyl-tRNA synthetase VFG1668 Protein 2e-85 41