Gene Information

Name : pchD (BPSS0584)
Accession : YP_110605.1
Strain :
Genome accession: NC_006351
Putative virulence/resistance : Virulence
Product : salicyl-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 792554 - 794197 bp
Length : 1644 bp
Strand : -
Note : Similar to Pseudomonas aeruginosa salicyl-AMP ligase PchD SWALL:Q9RFM9 (EMBL:AF184620) (547 aa) fasta scores: E(): 1.6e-152, 69.33% id in 538 aa, and to Bacillus subtilis 2,3-dihydroxybenzoate-AMP ligase DhbE or EntE SWALL:DHBE_BACSU (SWALL:P40871) (539 a

DNA sequence : 
TTGCCCGCCGCCGCGCGCAACGCCGCGTCGAGCGACGCGCCCGACTGGCCCGACGACTTCGCGCGCCGCTACCGCGCCGC
CGGCTACTGGCGAGACGAGACCTTCTACGGCGCGCTCGCCGATCGCGCGCGGCGCAGCCCCGCCGCGACCGCGGTCGTCG
ACGGCGAGCGCCGCGTCAGCTACCGCGCGCTGCTCGAGCGCATCCGGCGTCTCGCCGGCGGCTTACGCCGCCTGGGCCTC
GCGCGCGGCGACACGGCCGTCGTGCATCTGCCGAACGGCGTGCGCTTCATCGAAGCGTGCTTCGCGCTGTTCCAGCTCGG
CGTGCGCCCGGTGCTCGCGCTGCCCGCGCATCGGCAACACGAGATCGGCGCGTTCTGCCGCTTCACGAATGCCCGCGCAT
ATCTCGGCGCGGCCCGGCTCGGCGAGTTCGACTCCCACCCGCTCGCTCACGCGCTGCGGGCGAGCTGCCCGGCGCTCGCG
CACATCGTCGTCGCGGGCGACGATCATGCCTTCTTGCATTTCGATTCGCTGTACGACGCGCCGCCCGTCGCCGACTGCGC
GGCGCGCGCGGACGACATCGCGTGCTTCCAGCTCTCGGGCGGCACGACGGGCACGCCGAAACTGATTCCGCGCCGCCATC
GCGAGTACCTGTACAACGTGCGCGCGTGCTCGGACGCGAGCGGCTTCGGCGCCGACACCGTCTATCTGGCCGCGCTGCCG
ATGGCGCACAACTTCACGCTGTGCTGCCCCGGCGTGATCGGCGCGCTGCTCGCGGGCGGCCGCGTCGTCGCGACCGAGCA
CCCCGAGCCGGAGTGCGGCTTTGCGCTGATCGCGCGCGAGCGGGTCACGCATACGGCGCTCGTCCCGCCGCTCGCGCTAC
TGTGGCTGGACGCGCAGCGCGAGCGCCGAGCGGACCTGTCGAGCCTGCGCGTGCTGCAGGTCGGCGGCGCGCGGCTGATG
GACCACGCGGCCGAACGCGTGACGCCCGTCCTCGGCTGCCGGCTGCAACAGGTATTCGGAATGGCCGAAGGCCTCATCTG
CTGCACGCGGCTCGACGATCCGCCCGCGCGCATCGCGCGTACGCAGGGCCGGCCGGTGTCGCCCGCCGACGAAGTGCGCA
TCGTCGACGAGGCGGGCCGCGCCGTCGCGCCTGGCGAGATCGGCGAATTGCAGGTGCGCGGGCCCTATACGATCCGCGGC
TACTACCGGCTCGCCGAGCATCATGCGGCCGCGTTCACGGCCGACGGCTTCTATCGCAGCGGCGACCGCGTGCGCCGCAC
CGAAGAAGGCGACCTCGTCGTCGAGGGCCGCGACAAGGACCAGATCAATCGCGGCGGCGAAAAAGTATCCGCCGAGGAAG
TCGAGAACCTGCTGCTCGCGCATTCGCAAATCCGCGACGCGGCGCTCGTCGCGATGCCCGATCCGCTGCTCGGCGAGCGC
ACGTGCGCGTTCGTCGTCGCGCGCCCGCCCGCGCCGACCTCGCTCGCGCTGAAGCGGCATTTGCGCGACCACGGGCTCGC
CGCGTTCAAGATTCCCGATCGCATCGAATTCGTGCCGAGCTTTCCTGAAACCGGCATCGGCAAGACCAGCAAGAAATCGC
TGCGCGACCTGCTGCGCCGCCGGCTCGAAGCGGCGCGCGCATGA

Protein sequence : 
MPAAARNAASSDAPDWPDDFARRYRAAGYWRDETFYGALADRARRSPAATAVVDGERRVSYRALLERIRRLAGGLRRLGL
ARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQHEIGAFCRFTNARAYLGAARLGEFDSHPLAHALRASCPALA
HIVVAGDDHAFLHFDSLYDAPPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQRERRADLSSLRVLQVGGARLM
DHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIARTQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRG
YYRLAEHHAAAFTADGFYRSGDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMPDPLLGER
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRRRLEAARA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-100 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-100 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-100 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-100 50
ybtE CAA21394.1 - Virulence HPI Protein 2e-100 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-100 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-100 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-99 49
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-99 49

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pchD YP_110605.1 salicyl-AMP ligase VFG0168 Protein 8e-163 69
pchD YP_110605.1 salicyl-AMP ligase VFG0932 Protein 9e-97 46
pchD YP_110605.1 salicyl-AMP ligase VFG1817 Protein 1e-87 45