|
Name : lysS (MS1543) Accession : YP_088735.1 Strain : Mannheimia succiniciproducens MBEL55E Genome accession: NC_006300 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 1533383 - 1534894 bp Length : 1512 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCAGAACAACAAAATGCAGAATTAGATTTTCACGGTGAAATGGCGGTACGTCGCGAAAAATTAGCGGCCTTACGTGC AAAAGGTAACGCCTTTCCGAACACATTTCGCCGTGATGCATTAGCTCAGGATTTACATAATCAATACGATGAAACGGACG GCGAACAATTAAAAGAAAAAGATCTGCATGTTGCCGTTGCCGGTCGCATTATGACCCGTCGTACCATGGGAAAAGCGACT TTTATAACCATTCAGGATATGAGCGGTAAAATTCAGCTTTATGTGGCTCGCGATAACCTTCCTGAAGGCGTCTACGGGGA AGATGTAAAAAGCTGGGATTTAGGCGATATTGTCGGGATCAAAGGTACTTTATTTAAAACCAAAACCAACGAATTAACCG TAAAAGCTCATGAAGTGCAATTATTAACCAAAGCGCTTCGTCCGTTGCCGGATAAATTCCACGGTTTGAGCGATCAGGAA ACCCGTTATCGCCAACGTTATTTAGATTTAATTTCTAACGAAGAATCCCGTCGTACTTTTGTGATTCGCTCAAAAGTTAT CGCCGGTATCCGCGAATACTTCATCGGTAAAGGCTTCATCGAAGTAGAAACACCGATGCTGCAGGTAATTCCGGGCGGCG CGGCGGCGCGTCCGTTCGTAACTCACCACAACGCTCTGGATATCGATATGTACCTGCGTATCGCCCCCGAACTTTACTTA AAACGCTTAGTGGTGGGCGGCTTTGAACGGGTATTCGAATTAAACCGTAACTTCCGCAACGAAGGGGTTTCCGTGCGCCA CAACCCGGAATTCACTATGATCGAATACTATCAGGCATACGCGGATTACCACGATTTAATGGATAACACCGAAGAATTGT TACGTAAATTAGCCCTTGATATTTTAGGTACCACCATTGTGCCTTACGGCGAATATGAATTCGATTTCGGCAAACCGTTC GAACGTATCACAATGCATGATGCGGTGATTAAATACGGCGCGGAAAAAGGTATTGTAAAAGAAGATTTATACGATTTAGA CCGTGCCAAAGCGGCAGCCGCCAAATTAGGCATTGAAATCCAAAAATCCTGGGGCTTAGGTTCAATCGTCAATGCCATTT TTGAAGAAGTGGCGGAACATCACCTGATTCAACCGACATTCTTAATGGCTCACCCTGCGGAAATTTCGCCGTTGGCGCGC CGTAATGACGAAAACCCGGAAGTAACCGATCGTTTTGAATTATTCATCGGCGGCCGTGAAATCGGTAACGGTTTCTCGGA ATTGAACGACGCCGAAGACCAAGCTGAACGTTTCGACGCCCAAGTAGCGGCGAAAGACGCAGGCGACGATGAAGCCATGT TTAAAGATGACGACTTCGTCACCGCACTGGAACACGGCTTACCGCCGACTGCGGGCGAAGGTTTGGGGATCGACCGTTTA GCAATGCTGTTCGCCAACGCTCCGTCAATCCGCGATGTGATTCTATTCCCGGCAATGAAACACAAAGGGTAA Protein sequence : MSEQQNAELDFHGEMAVRREKLAALRAKGNAFPNTFRRDALAQDLHNQYDETDGEQLKEKDLHVAVAGRIMTRRTMGKAT FITIQDMSGKIQLYVARDNLPEGVYGEDVKSWDLGDIVGIKGTLFKTKTNELTVKAHEVQLLTKALRPLPDKFHGLSDQE TRYRQRYLDLISNEESRRTFVIRSKVIAGIREYFIGKGFIEVETPMLQVIPGGAAARPFVTHHNALDIDMYLRIAPELYL KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLALDILGTTIVPYGEYEFDFGKPF ERITMHDAVIKYGAEKGIVKEDLYDLDRAKAAAAKLGIEIQKSWGLGSIVNAIFEEVAEHHLIQPTFLMAHPAEISPLAR RNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDDDFVTALEHGLPPTAGEGLGIDRL AMLFANAPSIRDVILFPAMKHKG |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 2e-152 | 64 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 5e-89 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_088735.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 9e-153 | 64 |
