Gene Information

Name : pykF (MS1197)
Accession : YP_088389.2
Strain : Mannheimia succiniciproducens MBEL55E
Genome accession: NC_006300
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1194770 - 1196209 bp
Length : 1440 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGTCCAGAAGATTAAGAAGAACGAAAATCGTATGTACAATGGGGCCTGCAACAGACAAAGGCAATAATTTAGAAAAAAT
CATTGCTGCCGGTGCAAACGTTGTACGTATGAACTTCTCCCACGGTACGCCCGAAGATCATATCGGTCGTGCTGAAAAAG
TACGTGAAATCGCTCATAAATTAGGTAAACACGTAGCAATCTTAGGTGACTTACAAGGCCCTAAAATCCGTGTTTCTACT
TTTAAAGAAGGCAAAATTTTCTTAAATATCGGTGATAAATTCATTTTAGACGCAGAGATGCCTAAAGGTGAAGGTAACCA
GGAAGCGGTTGGTTTAGACTATAAAACATTACCGCAAGATGTGGTTCCGGGCGATATCTTATTATTAGATGACGGTCGAG
TTCAATTGAAAGTATTGGCGACCGAAGGTGCAAAAGTATTCACCGAAGTAACGGTCGGTGGCCCACTATCAAATAATAAA
GGCATTAACAAATTAGGCGGCGGTTTATCTGCCGATGCATTAACCGAAAAAGATAAAGCGGATATCATTACTGCGGCGCG
TATCGGGGTGGATTACCTTGCCGTATCTTTCCCGCGTTCAAGCGCGGATTTAAACTACGCCCGTCAATTAGCAAAAGATG
CGGGCTTGGATGCGAAAATTGTTGCGAAAGTAGAACGTGCCGAAACAGTTGAAACGGACGAAGCAATGGACGATATCATC
AATGCGGCGGACGTAATCATGGTTGCGCGCGGTGACTTAGGTGTTGAAATCGGTGACCCTGAATTAGTCGGTGTTCAGAA
AAAATTAATCCGTCGTTCACGTCAGTTAAATCGTGTTGTTATTACCGCAACTCAAATGATGGAATCAATGATTAGCAATC
CTATGCCGACTCGTGCGGAAGTAATGGACGTAGCTAACGCAGTATTGGACGGTACCGATGCGGTAATGCTTTCTGCTGAA
ACCGCGGCTGGTCAATATCCGGCGGAAACTGTTGCGGCGATGGCGAAAGTTGCGTTAGGTGCGGAGAAAATGCCAAGCAT
TAATGTGTCTAAACACCGTATGAACGTTCAATTCGAGTCTATTGAAGAATCTGTTGCAATGTCTGCAATGTATGCGGCAA
ACCACATGAGAGGCGTAGCGGCGATTATCACATTAACAAGTAGCGGTCGTACTGCTCGTTTAATGTCTCGCATTAGTTCC
GGTTTACCAATCTTTGCATTGTCACGTAACGAATCTACATTAAATTTATGTGCATTGTATCGTGGTGTGACACCGGTTCA
TTTTGATAAAGACAGCCGTACTTCAGAAGGTGCGAAAGCAGCGGTTCAATTATTAAAAGACGAAGGTTTCTTAGTGTCTG
GCGATTTAGTGTTATTAACTCAGGGCGACGCAAGCAGTTCTAGCGGTACTAACCTTTGCCGTACATTGATTGTTGAATAA

Protein sequence :
MSRRLRRTKIVCTMGPATDKGNNLEKIIAAGANVVRMNFSHGTPEDHIGRAEKVREIAHKLGKHVAILGDLQGPKIRVST
FKEGKIFLNIGDKFILDAEMPKGEGNQEAVGLDYKTLPQDVVPGDILLLDDGRVQLKVLATEGAKVFTEVTVGGPLSNNK
GINKLGGGLSADALTEKDKADIITAARIGVDYLAVSFPRSSADLNYARQLAKDAGLDAKIVAKVERAETVETDEAMDDII
NAADVIMVARGDLGVEIGDPELVGVQKKLIRRSRQLNRVVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMAKVALGAEKMPSINVSKHRMNVQFESIEESVAMSAMYAANHMRGVAAIITLTSSGRTARLMSRISS
GLPIFALSRNESTLNLCALYRGVTPVHFDKDSRTSEGAKAAVQLLKDEGFLVSGDLVLLTQGDASSSSGTNLCRTLIVE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 1e-75 48
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-55 41
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-56 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-55 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-55 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF YP_088389.2 pyruvate kinase VFG0479 Protein 4e-56 41