Gene Information

Name : lysS (BCZK0072)
Accession : YP_081689.1
Strain : Bacillus cereus E33L
Genome accession: NC_006274
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 80558 - 82057 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGATAACATGAACCACGAAGAATTAAACGACCAATTGCTTGTTCGTCGTGAAAAGCTACATAACTTACGTGAGCAAGG
AATCGATCCTTTCGGTAAACGATTTGAACGTACAAATGCAACAAATGACTTATTAAGCCTATATGGAGAGTTCTCTAAAG
AAGAATTAGAAGAGAAAGAAATCTCTGTTTCTATCGCTGGTCGTATTATGACAAAGCGTGGTAAAGGAAAAGCTGGATTT
GCACATATTCAGGATTTACATGGACAAGTTCAAATTTATGTTCGTAAAGATGCTGTTGGAGATGAAGAATACGAATTATT
TAAAACAGCAGATTTAGGCGACTTAGTAGGTATTGAAGGTAAAGTTTTTAAAACGAACGTTGGAGAACTTTCAGTGAAAG
CAACAGGCTTTACACTATTAACAAAATCTCTTCGTCCATTACCGGATAAATATCATGGGTTAAAAGATGTTGAACAACGC
TACCGTCAACGTTACTTAGACTTAATTACAAGTATGGAAAGCCGTGAAACATTCGTCACTCGCAGTAAGATTATTCGCGA
AATGAGAAGATACTTAGATGACAACGGTTATCTTGAAGTGGAAACACCTATGATGCATGCAATTGCAGGTGGAGCTTCTG
CTCGTCCTTTCATAACGCATCATAATGCGTTAGATATGGAATTATATATGCGTATCGCAATTGAACTTCATTTAAAACGC
CTTATTGTGGGTGGGTTAGAAAAAGTTTATGAAATCGGCCGTGTATTCCGTAATGAGGGTGTATCAACTCGTCATAACCC
TGAATTTACGATGATTGAATTATATGAAGCGTATGCTGATTATAAAGATATTATGAAACTAACAGAAAATATGGTTGCTC
ATATCGCGAAGCAAGTACTAGGTACAACAACAATCCAATATGGTGATTATGAAATTAATCTAGAACCAGAATGGACACGT
CTTCATATGGTAGATGCAATTAAAGAACATTCTGGAGCAGATTTCTGGAATCCGATGAGTGTAGAAGAAGCGCGTGAACT
TGCAAAAGAACATAATGTAGAAATTAAGGATACAATGGAAGTTGGTCATATTATTAATGAATTCTTCGAGCAAAAAGTAG
AAGATAAATTAATCCAACCAACATTTATTTATGGTCATCCGGTAGAAATTTCTCCACTTGCAAAAAAGAATGACGAAGAT
CCACGATTCACAGATCGTTTCGAATTGTTTATCGTTGCACGTGAACATGCAAATGCATTCACTGAGCTAAATGATCCAAT
CGATCAGAAAGAACGTTTTGAAGCGCAATTAAAAGAACGCGAACAAGGTAATGACGAAGCTCACATGATGGATGATGATT
ATATCGAAGCTCTTGAGTATGGCATGCCTCCTACAGGTGGACTAGGAATCGGTATTGATCGCCTTGTTATGTTATTAACG
AATGCACCATCTATTCGTGATGTATTATTATTCCCTGCTATGCGCCATAAACAAGACTAA

Protein sequence :
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKEISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-121 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_081689.1 lysyl-tRNA synthetase VFG1668 Protein 1e-121 51