Gene Information

Name : pykA (BCZK4339)
Accession : YP_085918.1
Strain : Bacillus cereus E33L
Genome accession: NC_006274
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 4454596 - 4456353 bp
Length : 1758 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGCGTAAAACTAAAATTGTATGTACTATAGGTCCTGCTAGTGAAAGTATTGAAAAATTAGAGCAATTAATCGAAGCAGG
TATGAACGTTGCTCGTTTAAACTTCTCTCATGGTAGCCATGAAGAGCACGGCGCTCGTATTAAAAACATTCGTGAAGCTT
CAAAGAAAACTGGTAAAACAGTTGGTATCTTACTTGATACAAAAGGTCCAGAAATCCGTACTCATGACTTCGTAGACGGA
CAAGCTGAGCTTGTAACAGGTGCAGAAGTAGTTCTTTCTACTGAGCAAGTATTAGGTACTGCAGAGAAGTTCTCTGTATC
TTATGCTGGTCTTTATGACGATGTAGATCCAGGTTCTCGCATTCTAATCGATGACGGTCTTATCGAGCTAGAAGTAATCG
AAAAAGCTGACGGAAACATCCGTACAAAAGTTCTTAACAGCGGAACTGTAAAAAATAAAAAAGGTGTTAACGTACCAAAC
GTAAGCATTAAGCTTCCTGGTATCACTGAAAAAGATGTAAAAGATATCATCTTCGGTATCGAGCAAAAAGTTGATTTCAT
CGCAGCTTCATTCGTACGTAAAGCAGCTGACGTATTAGAAATCCGTGAATTATTAGAAGAGCATAACGCTCAATACATCC
AAATCGTACCAAAAATTGAAAACCAAGAGGGTATTGACAACATCGATTCAATCTTAGAAGTTTCTGACGGCTTAATGGTA
GCTCGTGGTGACATGGGTGTAGAAATTCCGCCAGAAGAAGTACCATTAGTACAAAAACGTCTAATCAAAAAATGTAACGT
GTTAGGTAAGCCAGTTATTACTGCAACACAAATGTTAGATTCTATGCAACGTAACCCACGTCCAACTCGTGCGGAAGCAA
GTGACGTAGCTAACGCAATCTTCGATGGTACAGATGCAATCATGCTTTCAGGTGAAACGGCTGCTGGACAATACCCAGTA
GAAGCAGTAACAATGATGGCTAACATTGCAGTACGTGTTGAAAAATCATTACAATATGAAGATATGTTCAAAAAACGTAT
TAAAGAGTTCACTCCAACAATTACAGATGCAATTAGCCAATCTGTTGCGCATACAGCACTTGCTCTTGATGTAGCTGCAA
TCGTAGCTCCAACAGAAAGTGGATATACTGCGAAAATGATCTCTAAATACCGTCCAAAATCTCCAATCGTAGCTGTAACA
TCTGACGAGCAAGTAGGACGTCGTCTTGCACTTGTTTGGGGTGTACAAGCATTTATGGCTGAGAAGCGTGCAGCTTCTAC
TGACGAAATGTTAGATACAGCGATTCAAACAGGTATGGATGCAGGTCTAATCGGACTTGGAGACACTGTAGTAATCACTG
CTGGTGTACCAGTTGCTGAAACTGGTACAACAAACTTAATGAAAATCCACGTTGTTGGTGAAGAAGTTGCTAAAGGACAA
GGAATCGGTCGTAAAGCTGCAAAAGGTAAAGTAGTTGTAGCGAAAACAGCTGCTGAAGCTGTAGCGAACGTAAACGAAGG
TGATATCCTTGTTACAACAAGCACTGATAAAGATATGATTCCTGCAATCGAAAAAGCTGCTGCTCTAGTTGTAGAAGAAG
GCGGCCTAACAAGCCATGCGGCTGTTGTAGGTGTATCAATCGGTATTCCTGTTATCGTTGGTGTAAACGGCGTAACAGCA
ACTTTAAAAAATGGCCAAGAAGTAACAGTTGATGCAGCACGCGGAATTGTTTATAATGGACATGCGGAAGTGCTATAA

Protein sequence :
MRKTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGSHEEHGARIKNIREASKKTGKTVGILLDTKGPEIRTHDFVDG
QAELVTGAEVVLSTEQVLGTAEKFSVSYAGLYDDVDPGSRILIDDGLIELEVIEKADGNIRTKVLNSGTVKNKKGVNVPN
VSIKLPGITEKDVKDIIFGIEQKVDFIAASFVRKAADVLEIRELLEEHNAQYIQIVPKIENQEGIDNIDSILEVSDGLMV
ARGDMGVEIPPEEVPLVQKRLIKKCNVLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQYPV
EAVTMMANIAVRVEKSLQYEDMFKKRIKEFTPTITDAISQSVAHTALALDVAAIVAPTESGYTAKMISKYRPKSPIVAVT
SDEQVGRRLALVWGVQAFMAEKRAASTDEMLDTAIQTGMDAGLIGLGDTVVITAGVPVAETGTTNLMKIHVVGEEVAKGQ
GIGRKAAKGKVVVAKTAAEAVANVNEGDILVTTSTDKDMIPAIEKAAALVVEEGGLTSHAAVVGVSIGIPVIVGVNGVTA
TLKNGQEVTVDAARGIVYNGHAEVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-98 50
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-98 50
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-98 50
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 3e-98 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_085918.1 pyruvate kinase VFG0479 Protein 7e-99 50