Gene Information

Name : entE (BCZK2129)
Accession : YP_083720.1
Strain : Bacillus cereus E33L
Genome accession: NC_006274
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 2252232 - 2253848 bp
Length : 1617 bp
Strand : +
Note : -

DNA sequence : 
ATGTTAACAGGTTATACAAAATGGCCAAAAGAATTTGCCGATCGCTATCGAGAGGAAGGGTGTTGGCTTGGGGAAACATT
TGGCGGAGTGTTAAGAGAGCGTGCTGAAAAATATGGAGATCAAATTGCGGTTGTAAGTGGTAATACGTATATAACATATA
GTGAGCTTGATAAAAAAGTAGATCGCTTAGCGTCAGGTTTACTGAATTTAGGAATAAAGCCAGAAGATCGAGTTGTAATC
CAACTACCTAACGTTATCGAGTTTTTTGAAATGTGTTTTGCGCTATTTCGAATTGGAGCACTTCCTGTTTTTGCATTACC
TTCACATCGCAGTAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCGAGTGCGTACGTTATTTCAGATAAGGCTCTTG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGCAAGAGAAAGTACCAACTTTACAACATGTAATTGTAGTGGGAGAAGAA
GAAGAGTTTGTGAACATAAGTGATTTATATATAGATCCCGTTCCATTGCCAGAAGTTCAGCCAAGTGATGTTGCGTTTCT
CCAATTATCAGGAGGGACAACAGGACTTTCTAAATTAATTCCTAGAACGCATGATGACTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTAAGTGCAGAGAGCGTCTATATGGCAGTTCTTCCAGTAGCACATAATTACCCAATGAGTTCT
CCTGGAACATTTGGAACTTTTTATGCGGGTGGAAAAGTTGTGCTGGCAACTGGGGGTAGTCCGGATGAGACATTTGCACT
TATCGAAAAAGAAAAAGTTACGATTACTGCTCTCGTACCGCCATTAGCAATGATATGGCTTGACGCTGCATCTTTACGTA
ATAACGATTTATCAAGCTTAGAAGTGATTCAAGTAGGTGGTGCGAAATTTAGTGTTGAAGTTGCAAAACGTATACGTCCT
ACATTTGGATGTACATTACAGCAAGTGTTCGGTATGGCAGAAGGATTAGTGAACTATACAAGATTAGATGATCCGGAAGA
AATTATAATTCATACACAAGGTAGACCAATGTCTGCACTTGATGAAGTACGAGTTGTTGACGAATATGATAACGATGTAA
AACCTGGTGAAGTAGGTAGTTTATTAACACGGGGTCCATATACAATTCGTGGATACTATAAAGCAGAAGAGCATAATGCA
CGATCATTTACAAATGATGGATTTTATCGCACAGGAGATCTTGTAAAAGTAAACGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAATCGTGGTGGTGAGAAAGTCGCTGCGGAAGAAGTTGAAAATCATCTATTAGCACATGATG
CAGTACATGATGTAGCGATTGTATCTATGCCTGACGATTATTTAGGAGAGCGTACTTGTGCATTTGTAATAGCTCGCGGA
CAAGCTCCGGCAGTAAGTGAATTAAAAAGGTTCTTAAGAGAACGCGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGAGTCGGAAAAGTCAGTAAAAAGGAGCTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Protein sequence : 
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVQEKVPTLQHVIVVGEE
EEFVNISDLYIDPVPLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSLEVIQVGGAKFSVEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTNDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-125 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-126 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-125 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-125 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-125 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-125 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-125 50
ybtE CAA21394.1 - Virulence HPI Protein 2e-125 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-126 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_083720.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 3e-140 52
entE YP_083720.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 2e-116 48
entE YP_083720.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 2e-116 45