Gene Information

Name : clpB (YPTB0647)
Accession : YP_069189.1
Strain : Yersinia pseudotuberculosis IP 32953
Genome accession: NC_006155
Putative virulence/resistance : Virulence
Product : ATP-dependent protease, Hsp 100, part of novel multi-chaperone system with DnaK, DnaJ, and GrpE
Function : -
COG functional category : O : Posttranslational modification, protein turnover, chaperones
COG ID : COG0542
EC number : -
Position : 766349 - 768952 bp
Length : 2604 bp
Strand : +
Note : similar to Yersinia pestis YPO0506 clpB, htpM; Clp ATPase (100% evalue=0); Pseudomonas aeruginosa PA0090 probable ClpA/B-type chaperone (44.4% evalue=0)

DNA sequence :
ATGGGAGCGATAACGGTGTATCAACGCGAACGTCTTTTTAATCGATTGGGCACTTTTGCCTATAAAACTTTTGTTGAAGC
CACCCGTCTTTGCCGCAGTTATCGGCATGAGTATGTGGAGCTTGAACATTGGTTAAAAATTCTCATGGATCAACAGCGTG
GGGACATTCCTGCTCTACTGACGCATTACGGACTGAGCCAAACGGTTATCTCCACGCAATTGGATCGCATTATTCAGCAT
ATGCCCGTCACCAAAGCATCGGTGCAGGATTTATCATCACGCTTGGAGTCCGTGGTCGAAGGCGGATTGGTGTTGAGCCA
ATTGATGGCGTCACCTTCTCCTATCCGTACCTCACATATTTTACTGGCACTATTACAGGATACCCAGCATCAGCGCTGGT
TATACCGCCTGTGCGATGAATTCAAAAAGCTGCCGGTCCCACAGGTTGCAGAGGAATATGAAGGCCTATTAGTTAACTCC
ATTGAACAGACCAGCGAGGTTCAAAGAGGCGACGCATTAGTGACGGAAACGGACAGCAGCTCATCAAAAGAAGCGCATGC
GGCATTGAACAAATGGTGTAACGACCTTACCCAGCAAGCCCGTGACGGTGAGATTGACCCCGTTATTGGTCGTGAAGCAG
AACTGCGTCAAGTGATTGATATTTTACTCCGTCGCCGTCAGAACAACCCTATTTTGGTTGGGGAAGCGGGGGTCGGTAAA
ACGGCGGTGGTTGAAGCGCTAGCGCGAAAACTGGCCAGTGGCGATGTCCCTCCGCTGTTGCAAGGGGCGCGTCTGCTTTC
TCTGGATTTGGGGAGGATGCAAGCCGGTGCCAGTATGCGCGGTGAATTTGAATCACGTCTTAAATCCCTGATCGATGGCA
TCAGCCAATCGGATACCCCGGTTATTCTGTTTTGTGATGAAGCCCATACGTTGGTGGGGGCAGGCGGGCAGGCGGGCACG
GGGGATGCGGTCAACCTGCTTAAACCGATGTTGGCGAGGGGCGCATTGCGCATGATCGCCGCCACCACCTGGTCAGAGTA
TAAACAGTTTATTGAACCTGACGCCGCGCTGACCCGGCGTTTCCAGCGGGTATTGATTGGCGAGCCAAACGAATCTACCG
CCGTCGATATGCTACGGGCGATTGCGCCACATTTTGCTCAACATCATAACGTCACTATCCGCGACGGTGCCATTCACGCG
GCGGTGCGCCTGTCATTACGCAACCTGCCTTCCCGGCAGTTGCCCGATAAAGCCATCAGTCTGCTCGACACCGCTTGCGC
GCGGGTCGCGCTCAGCCAATATGCCCAACCACAGGCGATTGAACAGTTATCGGCCCAACTGGATGTGCTTAAAACCGAGC
ATCAATATCTGGTACGGGAGAAGCAACTGGGCGAAGCGGTTGATCAACGGCTAGATGACGTTCAGACACAGATCCAAGCG
TTTGAACATGAACTAACCGCCTTGCAAAGCCGCCATCTGCATGAGCAACAGCTAGTGAGGGAGGTCCTGCCGGAGGGAGA
AGGCAGTGGCATGATGACGGAAAATGCCCGCTGGGGTGAACTGATGGCGTTACAGGAAGCGTCGCCACTGGTCTATCCAT
GGGTTGATGAGCAGAGCATCGCCGCGGTGCTTTCCGATTGGACCGGGATCCCGTCCGGCAAAATGTTGCAGGACGATATT
GAATGCGTCCTGAATCTTGAACAGCGTCTCGGTGATCATATTTTCGGGCAACGCAACGCTATCAAAGAGATTTCGCAGGC
GATACGTATTGCTCGGGCGGGCATCCAGTCTCAGGAGCGGCCACTCGGGATCTTCCTGCTGGCGGGGTCAACCGGTACGG
GCAAAACAGAAACGGCCAATGTACTGGTCGAAACCTTGTATGGCGGTGCTCATAACCTTATTACCTTCAATATGAGTGAG
TTCCAAGAGGCGCATACCTTATCCACGCTGAAAGGCGCACCTCCGGGCTATGTCGGCTATGGCAAGGGGGGGAAATTAAC
CGAAGCGGTAAGACGCAAACCTTATTCGGTCATCCTGCTTGATGAGTTTGATAAAGCTCACGCCGATATTCAGGACGCCT
TTTATCAGGTCTTCGACAAAGGGTGGATGGAAGATGCCGAAGGGCGTCGGGTCAGTTTCCGCCAGTGTTTCATCTTACTG
ACCTGTAATCAGGGCGCGGAAGAGATCGAACAGGCTTACCTCACCGCCAACGATATCAAACCCGGTGCATTAAAACCGTT
GGTATACGACGCCCTGTTGCGCCGCTTTGCGCCAGCGCTGTTAGCCAGGGTCAATATCATTCCTTATATCCCATTAGATC
AGGACGCTCTGGCACAGATCGCCTCTCATCATCTTGCGCGCTTACAAACGCGCCTGTGGGATGAAATTGGTGCCACTTTG
GTGACCGAGGGGGATATCCCCGGCTGGATCGCCTCACGGGTTTGCAGCCATCCGAACCATGGTCGTGCGGTGGAAGAACT
CTTGCGTCAAACCTTACTTCCCGCTGTTGGCAATGAAGTGTTGAAGCGTCGCCATGAGGCTGAACCGCTGAGAGAGATAC
GCCTGATTGTTGAGGAGACAGAACTGAGTATTGAGTTCGCGTAG

Protein sequence :
MGAITVYQRERLFNRLGTFAYKTFVEATRLCRSYRHEYVELEHWLKILMDQQRGDIPALLTHYGLSQTVISTQLDRIIQH
MPVTKASVQDLSSRLESVVEGGLVLSQLMASPSPIRTSHILLALLQDTQHQRWLYRLCDEFKKLPVPQVAEEYEGLLVNS
IEQTSEVQRGDALVTETDSSSSKEAHAALNKWCNDLTQQARDGEIDPVIGREAELRQVIDILLRRRQNNPILVGEAGVGK
TAVVEALARKLASGDVPPLLQGARLLSLDLGRMQAGASMRGEFESRLKSLIDGISQSDTPVILFCDEAHTLVGAGGQAGT
GDAVNLLKPMLARGALRMIAATTWSEYKQFIEPDAALTRRFQRVLIGEPNESTAVDMLRAIAPHFAQHHNVTIRDGAIHA
AVRLSLRNLPSRQLPDKAISLLDTACARVALSQYAQPQAIEQLSAQLDVLKTEHQYLVREKQLGEAVDQRLDDVQTQIQA
FEHELTALQSRHLHEQQLVREVLPEGEGSGMMTENARWGELMALQEASPLVYPWVDEQSIAAVLSDWTGIPSGKMLQDDI
ECVLNLEQRLGDHIFGQRNAIKEISQAIRIARAGIQSQERPLGIFLLAGSTGTGKTETANVLVETLYGGAHNLITFNMSE
FQEAHTLSTLKGAPPGYVGYGKGGKLTEAVRRKPYSVILLDEFDKAHADIQDAFYQVFDKGWMEDAEGRRVSFRQCFILL
TCNQGAEEIEQAYLTANDIKPGALKPLVYDALLRRFAPALLARVNIIPYIPLDQDALAQIASHHLARLQTRLWDEIGATL
VTEGDIPGWIASRVCSHPNHGRAVEELLRQTLLPAVGNEVLKRRHEAEPLREIRLIVEETELSIEFA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY0294 NP_454876.1 ClpB-like protein Not tested SPI-6 Protein 8e-148 45
aec27 YP_851418.1 ATPase Not tested PAI II APEC-O1 Protein 2e-107 41
aec27 AAQ96721.1 Aec27 Not tested AGI-1 Protein 2e-107 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
clpB YP_069189.1 ATP-dependent protease, Hsp 100, part of novel multi-chaperone system with DnaK, DnaJ, and GrpE VFG2076 Protein 4e-156 44
clpB YP_069189.1 ATP-dependent protease, Hsp 100, part of novel multi-chaperone system with DnaK, DnaJ, and GrpE VFG2084 Protein 2e-123 41