Gene Information

Name : lysS (YPTB3163)
Accession : YP_071665.1
Strain : Yersinia pseudotuberculosis IP 32953
Genome accession: NC_006155
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3719685 - 3721202 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCAGAGCAAAAACCACAAGTCGCTGAGCAAGCGCAAGAACTCAATAGTGAGTTACAAGCCCGTCGAGAAAAACTGGC
TGTCTTACGGGGAAAGGGAATTGCATTCCCTAATGATTTCCGCCGTGAGAACCTTTCCGACCAGTTGCATGCCGAGTTTG
ACAGTAAAGAGAATGAAGAGTTAGAAGCACTGAATATCGACGTAACCGTTGCTGGCCGTATGATGACCCGCCGTATCATG
GGTAAAGCATCATTTGTGACTCTGCAAGATGTTGGTGGCCGTATCCAGTTGTACGTTTCCCGTGATGACCTACCGGAAGG
CGTTTATAACGAAGAATTCAAAAAGTGGGACCTGGGCGATATCCTGGGTGCGCGCGGTAAACTGTTTAAAACCAAAACCG
GTGAGCTGTCGATCCACTGTAGCGAACTGCGTCTGTTGACCAAGGCTCTGCGTCCATTGCCAGATAAATTCCATGGCTTA
GCTGACCAGGAAACCCGTTACCGCCAGCGTTATTTGGATCTGATTGCTAACGACGAGTCTCGTCATACTTTCAAAGTCCG
TTCGCAAGTGATGTCCGGTATCCGTAGCTTCATGGTTGAAAAAGGCTTCATGGAAGTCGAAACGCCAATGATGCAAGTGA
TCCCTGGCGGCGCTTCCGCACGTCCGTTTGTCACGCATCACAATGCGTTGGATATCGATATGTATTTGCGTATCGCGCCA
GAGCTGTATCTGAAACGTCTGGTCGTGGGGGGCTTCGAACGTGTGTTCGAAATCAACCGTAACTTCCGCAACGAAGGGGT
TTCGCCACGTCATAACCCAGAGTTCACCATGATGGAACTCTATATGGCTTATGCGGACTATAAAGACCTGATCGTCTTGA
CGGAAGAGCTATTCCGCACTCTGACCGAAACCATATTGGGGAGCAGTGTGGTGCAATATGGCGAACAGACTTTTGATTTC
GGTAAGCCTTTTGCCAAATTGACCATGAAAGAAGCTATTTGTAAATATCGTCCTGAAACCAATGTCGCGGATTTAGACGA
TATGGATAAAGCCGTCGCAATTGCCGAATCATTGGGTATCAAGGTTGAGAAGAGCTGGGGCCTGGGTCGTATTCAATGTG
AAATCTTTGAAGAGACCGCAGAAAGTCATCTTATCCAGCCAACGTTCATCACAGAATATCCAGCCGAGGTTTCTCCGCTA
GCACGCCGTAATGACGATAATCCATTTATCACTGACCGCTTTGAATTCTTCATCGGTGGCCGTGAAATTGGTAATGGCTT
CTCTGAGTTGAACGATGCCGAAGACCAGGCGCAGCGTTTCGCCGATCAGGTAAGTGCTAAAGAAGCTGGCGATGATGAAG
CGATGTTCTACGATGAAGACTATATCACTGCATTGGAACATGGCTTACCACCAACCGCAGGGCTAGGTATTGGTATTGAC
CGTATGGTTATGTTGTTTACCAATAGCCATACCATCCGTGATGTCATTTTATTCCCAGCGATGCGCCCGGTAAAATAA

Protein sequence :
MSEQKPQVAEQAQELNSELQARREKLAVLRGKGIAFPNDFRRENLSDQLHAEFDSKENEELEALNIDVTVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVSRDDLPEGVYNEEFKKWDLGDILGARGKLFKTKTGELSIHCSELRLLTKALRPLPDKFHGL
ADQETRYRQRYLDLIANDESRHTFKVRSQVMSGIRSFMVEKGFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTEELFRTLTETILGSSVVQYGEQTFDF
GKPFAKLTMKEAICKYRPETNVADLDDMDKAVAIAESLGIKVEKSWGLGRIQCEIFEETAESHLIQPTFITEYPAEVSPL
ARRNDDNPFITDRFEFFIGGREIGNGFSELNDAEDQAQRFADQVSAKEAGDDEAMFYDEDYITALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-164 67

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_071665.1 lysyl-tRNA synthetase VFG1668 Protein 6e-165 67