Gene Information

Name : lysS (Mfl030)
Accession : YP_053270.1
Strain : Mesoplasma florum L1
Genome accession: NC_006055
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 41444 - 42943 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCAGAAAGAAAATTTACTGAACAAGAATTAGTTCGTAGAGCTAAACTTGAAAAATTAATTGAAAACAAATCTAATCC
TTATGAAGTTAGCAAGTATGTAAGAACACATTCTTTAAAAGAATTGATTGAATTATTTGGTGGATATGAAAAAGATGAGT
TAGCAACTTTACAACAAACAGAAATTAGCATTGCAGGAAGAATTAAACTTTATCGTGAGGCTGGTAATAAAGCAGCATTC
TTAAATATTGAAGATCAAGATTCAGATATTCAAGTTTATGTAAGACAAGACGAAATTGGTGATGCAGCATTTGCTAACTT
CAGAGAACTAGATCTTGGAGATATTGTTGGGTTTAAAGGAATTATGATGAAAACAAACCATGGTGAACTTTCACTAAGAG
CAAAAGAATATACTTTATTATCAAAAGCTTTAAGACCTTTACCAGATAAACATGCTGGTATTCAAGACATTGAAGAAAAA
TACAGAAGAAGATATGTTGACTTAATCACAAACCCTGAATCAAAAAGAGTTTTTCAAGCAAGAACTAAAATTATTAGAAC
AATGCAACAAATTCTTGATGAACGTGGGTATATGGAAGTTGAAACACCTATTTTGCACAGTGTAAAAGGTGGAGCCGCTG
CTAAACCTTTTATTACACATTACAATGCTTTAGATGTTGATGTATATTTAAGAATTGCAACAGAGCTACATTTAAAAAGA
TTAATTGTTGGTGGATTTGATGGAGTTTATGAAATTGGAAGAATCTTTAGAAATGAAGGAATGAGCACAAGACACAATCC
AGAATTTACTTCTTTAGAATTGTATGTTGCATATGAAGATATGTTCTTCTTAATGGACTTAACTGAAGAAATATTCAGAA
AATGTAATGCTGCAGTTAACGAATCATCAGTTATAGAATATGGTGGACATAAACTAGATTTATCAAAACCATTTAAACGC
TTACATATGGTTGATGGAATTAAACAAGTGACAGGAGTAGATTTCTGAAAAGAAATGACTGTTGAAGAAGCTCTAGAGTT
AGCTAAAAAACATAATGTGCATGTTGAAAAACATAATTATACAGTTGGACATATTATTAACTTATTCTATGAAGAATTTG
TTGAGTCTACAATTATTGAACCAACTTTTGTTTATGGACATCCAAAAGAAATATCACCTTTATCTAAATCGAATGCTGAA
GATTCAAGATTCACTGATCGTTTTGAGTTGTTTATTGTCGGAAGAGAATATGCAAATGCATTTAGTGAATTAAATGATCC
AATCGATCAATATGATAGATTTAATGAACAAATAAAAGAAGCTGCGGCGGGAAATGATGAAGCAACTGAAATGGACATTG
ACTTTATTGAAGCGTTGGAACACGCAATGCCACCAACTGCAGGTATCGGATTTGGAATTGATCGTTTAGTTATGTTATTA
ACAAATTCTGAATCAATTAAGGATGTATTATTATTCCCACAGTTAAAACCAAGAGATTAA

Protein sequence :
MSERKFTEQELVRRAKLEKLIENKSNPYEVSKYVRTHSLKELIELFGGYEKDELATLQQTEISIAGRIKLYREAGNKAAF
LNIEDQDSDIQVYVRQDEIGDAAFANFRELDLGDIVGFKGIMMKTNHGELSLRAKEYTLLSKALRPLPDKHAGIQDIEEK
YRRRYVDLITNPESKRVFQARTKIIRTMQQILDERGYMEVETPILHSVKGGAAAKPFITHYNALDVDVYLRIATELHLKR
LIVGGFDGVYEIGRIFRNEGMSTRHNPEFTSLELYVAYEDMFFLMDLTEEIFRKCNAAVNESSVIEYGGHKLDLSKPFKR
LHMVDGIKQVTGVDFWKEMTVEEALELAKKHNVHVEKHNYTVGHIINLFYEEFVESTIIEPTFVYGHPKEISPLSKSNAE
DSRFTDRFELFIVGREYANAFSELNDPIDQYDRFNEQIKEAAAGNDEATEMDIDFIEALEHAMPPTAGIGFGIDRLVMLL
TNSESIKDVLLFPQLKPRD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 9e-101 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_053270.1 lysyl-tRNA synthetase VFG1668 Protein 5e-101 45