Gene Information

Name : Mfl175 (Mfl175)
Accession : YP_053416.1
Strain : Mesoplasma florum L1
Genome accession: NC_006055
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 203834 - 205270 bp
Length : 1437 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGAACAAGAAAGAATTACAAGCACGTGTAAAACGTACTAAAATTATTACTACAACTGGTCCATCAACAAATGAACCAGA
ACAAATAAGAGAATTATTTGAAAATGGAATGACAACAATACGTTTAAATTTTTCACATGGTGATTATGAAGAACAAGGTT
ACAGAATTGCTGGAGCTAAAAAAGTTAGAGAAGAATTAGGAAAACCTGTTTCTATTTTATTAGATACAAAAGGGCCTGAA
ATTCGTGTTGGTAAATTTGTAGATGGAAAACAAGAAGTTACTGCAAATCAATCAATTACAATTTACACAGATGCTGAATC
ATTCAAAAACAAAGAATGTTTATCAGGAGAAATGACTGTTGCTTATGACATGTCAGTTGATTTAAAAATTGGTGATACAA
TTTTAATCGATGATGGTAAATTAGAAATGACTGTTGAAGAAGTTAAACCAGGTGTTGTTAAAGCTATCGCTTTCAATAAC
CACTTAGTAAAAACTAACAAACGTGTTAACTTACCAGGTGTTGATTTCTCAATGCCATTCTTAGCTCAAAAAGATATCAA
TGATATTAAATATGGAGTTGAACAAGGTGTTGACTACATCGCTGCATCATTTGTTAACTCAGCTGAAAATGTAAAAGAAA
TTCGTGATATTTTAGCAGAAGCTAATGGATCAGATATTCAAATTATTTCAAAAATTGAATCACAAGTTGGAATTGACAAC
ATTGATGCAATTATTGAAGCATCAGATGGAATTATGATTGCTCGTGGTGACTTAGGATTAGAAATTCCTTACTACGACGT
ACCATACTGAGAAAAAATAATGATCAGAAAATGTCGTGAAGCTGGTAAAGTTGTTATTGTTGCTACTCAAATGTTAGAAA
CAATGACAGAAAACCCAGCACCTACAAGAGCTGAAGTAACTGACGTTTACTTTGCTACTGAATTAGGAGCAGATGCAACA
ATGTTATCTGGAGAATCAGCAGCTGGAGATTACCCATTCATTACTGTTAACACAATGGCAACAATCAACAAACGTGCTGA
AATAGAATTCTACAAAAAAGCATACTACCAAACTCAATTAGAAAACGCTAAAAACTCAACTAGTGGACCACGTGCAGATA
TTGCAATGGACTTAGCTGAAAGAACTCGTGATGGACAATATGAATTTGCTGTTGTTTTATCAAGAACTGGAGCTTTATTA
AAAACTATTTCTAAATTTAGACCAAATGTAACAATTTTAGGTGTAAGTGAATCAGAAAGATTATGAACTGCATTTGGTGT
ATGACACTCAATCTTCATGAACAAAACTGCTAACTTATTAGAATTAGAAGAAAATGTTGCTCAAATCTCAGAAATTGCAA
AATTATGAGGTGCAAAATCAGGATCAAAAATCTTAGTTGTAAGAAACAAAGATATTCGTGAAATTACAGTAGCTTAA

Protein sequence :
MNKKELQARVKRTKIITTTGPSTNEPEQIRELFENGMTTIRLNFSHGDYEEQGYRIAGAKKVREELGKPVSILLDTKGPE
IRVGKFVDGKQEVTANQSITIYTDAESFKNKECLSGEMTVAYDMSVDLKIGDTILIDDGKLEMTVEEVKPGVVKAIAFNN
HLVKTNKRVNLPGVDFSMPFLAQKDINDIKYGVEQGVDYIAASFVNSAENVKEIRDILAEANGSDIQIISKIESQVGIDN
IDAIIEASDGIMIARGDLGLEIPYYDVPYWEKIMIRKCREAGKVVIVATQMLETMTENPAPTRAEVTDVYFATELGADAT
MLSGESAAGDYPFITVNTMATINKRAEIEFYKKAYYQTQLENAKNSTSGPRADIAMDLAERTRDGQYEFAVVLSRTGALL
KTISKFRPNVTILGVSESERLWTAFGVWHSIFMNKTANLLELEENVAQISEIAKLWGAKSGSKILVVRNKDIREITVA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-70 44
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-70 44
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 9e-71 44
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-70 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Mfl175 YP_053416.1 pyruvate kinase VFG0479 Protein 1e-70 44