Gene Information

Name : megL (BT9727_4388)
Accession : YP_038703.1
Strain : Bacillus thuringiensis 97-27
Genome accession: NC_005957
Putative virulence/resistance : Resistance
Product : methionine gamma-lyase
Function : -
COG functional category : E : Amino acid transport and metabolism
COG ID : COG0626
EC number : 4.4.1.11
Position : 4451425 - 4452603 bp
Length : 1179 bp
Strand : -
Note : catalyzes the formation of methanethiol and 2-ocobutanoate from L-methionine

DNA sequence :
ATGAAAAAGAAGCATATGGAGACAGCGTTAATTCATCACGGTTATACATCAGAGGAACATAAAGGAAGTTTAACACCGCC
TTTATTTCAAACGTCTACATTTACATTTGAGACTGCGCAGCAAGGAGAGGCGAGTTTTGCGGGAGTGGATCCATCTTATA
TTTACTCAAGACTTGGAAATCCGACTGTGAAATTATTTGAAGAACGTATGGCGGTGTTAGAAGGTGGAGAAGAAGCACTT
GCTTTCGGATCCGGAATGGCAGCTATTTCAGCTACTTTAATTGGTTTTCTAAAAGCTGGAGATCATATTATTTGTTCAAA
TGGATTATACGGATGCACGTACGGCTTTTTAGAAGTGTTAGAAGAAAAATTTATGATTACGCATTCGTTTTGTGATATGG
AGACAGAGGGTGATATTGAAAATAAAATTTGCCCAAATACAAAGCTTATTTTCGTTGAAACACCAATTAATCCGACAATG
AAACTAATTGATTTAAAACAAGTGATTCGGGTTGCGAAGCGAAATGGGTTACTTGTCATTGTTGATAATACGTTTTGTTC
ACCTTATTTACAAAGACCTCTTGAACTTGGCTGTGACGCGGTTGTTCATAGTGCGACAAAATATATTGGTGGCCACGGTG
ATGTTGTGGCGGGTGTAACAATTTGTAAAACGAGAGCGTTAGCTGAAAAAATCCGCCCGATGCGAAAAGATATCGGCGGT
ATTATGGCACCGTTTGATGCATGGTTATTGTTACGCGGATTAAAGACACTAGCGGTAAGAATGGACCGCCATTGTGATAA
TGCAGAAAAAATTGTATCGTTCCTAAAAAATCATGATGCGGTAGAAGGTGTTTGGTATCCAGAAGGGGAGTTAGCATCTC
GCCAAATGAAACGGGGCGGCGGCGTTATATCTTTTTCAATTAAAGGCGGGAAAGAAGAGACGCAAGCGTTTATTAATGAC
CTCTACTTTATTACAATTGCAGTCAGTTTGGGAGATACAGAAACGTTAATTCAGCACCCGGCAACGATGACACACGCTGC
GATTCCAGCTGAGTTAAGACAAGAAATGGGTATTTATGATAATTTAATACGTTTATCTGTCGGTTTAGAATCGTGGGAGG
ATATCGTTTCTGATTTAGAGCAAGCATTAAAGAAAATATCTACTGTTAGTAATCAATAA

Protein sequence :
MKKKHMETALIHHGYTSEEHKGSLTPPLFQTSTFTFETAQQGEASFAGVDPSYIYSRLGNPTVKLFEERMAVLEGGEEAL
AFGSGMAAISATLIGFLKAGDHIICSNGLYGCTYGFLEVLEEKFMITHSFCDMETEGDIENKICPNTKLIFVETPINPTM
KLIDLKQVIRVAKRNGLLVIVDNTFCSPYLQRPLELGCDAVVHSATKYIGGHGDVVAGVTICKTRALAEKIRPMRKDIGG
IMAPFDAWLLLRGLKTLAVRMDRHCDNAEKIVSFLKNHDAVEGVWYPEGELASRQMKRGGGVISFSIKGGKEETQAFIND
LYFITIAVSLGDTETLIQHPATMTHAAIPAELRQEMGIYDNLIRLSVGLESWEDIVSDLEQALKKISTVSNQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SAS0418 YP_042545.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 2e-66 43
SAR0460 YP_039909.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 4e-65 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
megL YP_038703.1 methionine gamma-lyase BAC0207 Protein 9e-89 48