Gene Information

Name : ddl (PSPPH_3913)
Accession : YP_276049.1
Strain : Pseudomonas syringae 1448A
Genome accession: NC_005773
Putative virulence/resistance : Resistance
Product : D-alanyl-alanine synthetase A
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 4472400 - 4473491 bp
Length : 1092 bp
Strand : -
Note : D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli

DNA sequence :
TTGAAAAAGTCAGTCGCCATCGTTTTCGGTGGGCAATCCAGCGAGCATGAGGTTTCGTTGCAGTCGGCACGCAACGTGAT
CAATGCGATTGATCGTGAACGCTATACGCTGACCCTGATCGGCGTCGACAAGCTGGGCCGCTGGTTGCGCTTCGACGAGG
CCGACTACCTGCTCAATGCCAATGATCCTGCGCGGATCAAATTGAGTGCATCCGGCAAGCAGTTGTCGCTGTTGCCTGGC
AGCACTGGTGGCCAGTTTGTCGAGGTCGAGACAGGCAAGCCACTGGCGGCCATTGACGTGGTATTTCCGCTGATCCACGG
CGCGTTTGGTGAGGACGGTGCGTTGCAAGGCGTGCTGCGCATGCTGGCCGTTCCGTTTGTCGGCGCTGACGTCCTCGCCT
CGGCGGCGTGCATGGACAAAGACGTGACCAAGCGTCTGCTGCGCGATGCAGGCATTGCTGTCGCGCCGTTCATGGTGCTC
AGCCGTGGGGAAACCCTGTCGTTCGCCGATGCAGCCACGCAATTGGGCCTGCCGATGTTCGTCAAGCCGGCCAATCAAGG
CTCGTCGGTGGGCGTCAGCAAAGTGTCTGACGAGGCTGGCTTCGTGGCTGCGCTGGCATTGGGTTTCGAGTTTGACCACA
AGCTGCTGATCGAGCAGGGCATCGTCGGCCGGGAAGTGGAGTGCGCCGTACTGGGTAACTTCGATGCGCAGGTCAGTGTC
TGTGGCGAAGTGATTGCCAACGACGAGTTCTACGCCTACGACACCAAATACCTGAATGGTGATCAGGCGCGCATCGCGAT
ACCCGCCGAGTTTCCTGCCGATCTGAGTGATCAGGTGCGTGAAGTCGCGTTGCAGGCTTACAAGGTGCTGGGTTGCGCAG
GGCTTTCGCGGGTCGATTTCTTCGTTACCGACACGCGTGACATCATCATCAATGAGGTCAACACGCTGCCTGGCTTCACT
TCCATCAGCATGTACCCCAAGCTCTGGCAGGCCAGTGGTCTGACCTATGCCGAACTGATCCATCGCCTGATCGTGCTGGC
CCTTGAGCGCGCTGATCAGGCGCGTTTGCTGAAAACGGAAATCTTTGCATGA

Protein sequence :
MKKSVAIVFGGQSSEHEVSLQSARNVINAIDRERYTLTLIGVDKLGRWLRFDEADYLLNANDPARIKLSASGKQLSLLPG
STGGQFVEVETGKPLAAIDVVFPLIHGAFGEDGALQGVLRMLAVPFVGADVLASAACMDKDVTKRLLRDAGIAVAPFMVL
SRGETLSFADAATQLGLPMFVKPANQGSSVGVSKVSDEAGFVAALALGFEFDHKLLIEQGIVGREVECAVLGNFDAQVSV
CGEVIANDEFYAYDTKYLNGDQARIAIPAEFPADLSDQVREVALQAYKVLGCAGLSRVDFFVTDTRDIIINEVNTLPGFT
SISMYPKLWQASGLTYAELIHRLIVLALERADQARLLKTEIFA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 1e-49 41
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 9e-52 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ddl YP_276049.1 D-alanyl-alanine synthetase A U00456.gene.p01 Protein 5e-50 41
ddl YP_276049.1 D-alanyl-alanine synthetase A AY145441.gene.p01 Protein 8e-51 41
ddl YP_276049.1 D-alanyl-alanine synthetase A U35369.1.gene6.p01 Protein 5e-50 41
ddl YP_276049.1 D-alanyl-alanine synthetase A AE016830.1.gene2250. Protein 5e-50 41
ddl YP_276049.1 D-alanyl-alanine synthetase A AF310956.2.orf5.gene Protein 6e-51 41