Gene Information

Name : topB1 (PSPPH_2943)
Accession : YP_275124.1
Strain : Pseudomonas syringae 1448A
Genome accession: NC_005773
Putative virulence/resistance : Unknown
Product : DNA topoisomerase III
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0550
EC number : 5.99.1.2
Position : 3414939 - 3416912 bp
Length : 1974 bp
Strand : +
Note : decatenates replicating daughter chromosomes

DNA sequence :
ATGCGGCTGTTTCTTTGCGAAAAACCCTCTCAGGCCAAAGACATTGCGGCTGCGCTCGGCGCGACCCGGCGTGGCGACGG
CTGCTGGGTGGGTGCCAATGCCACCGTAACCTGGTGCATCGGCCACTTGCTGGAAACCGCCCCGCCCGACGCCTACGATG
CGCGTTACAAACGCTGGGTGCTGGAAGACCTGCCCATCATTCCGGAAAAGTGGAAGATGCTCGTCAAGCCGAGAACTGCC
AGCCAGTTCAAGGCAGTCAAGCGATTGCTGGGCGAAGCTCAAGAGTTGGTGATAGCCACCGATGCCGACCGCGAAGGCGA
GATGATCGCCCGCGAACTGGTCGAACACTGTCGCTATCGCGGGCCGATTCAGCGTTTGTGGCTGTCGGCACTCGATGACG
CCTCCATCCGCAAGGCGCTGGCAGCGCTCAAACCCGGTGCCGATACGTTCAGCCTGTATCACTCGGCACTGGGTCGATCC
CGTGCCGACTGGCTGATCGGCATGAACATGAGCCGCCTGTTCACCCTGCTGGGTCGCCAGTCGGGTTATCAGGGCGTATT
GCCAGTCGGGCGGGTACAAACCCCGACCCTGCGGCTGGTGGTGGACCGTGATCGCAGCATCGCCAACTTCGTACCGGCAC
CCTACTGGGCCATTGACGTCAGGCTGCTGCACGACAACCAGGCTTTTATCGCCCAATGGCGCGCACCGTCCGACGCCTGC
GACGATCAGGACCGCTGCCTGAATCAGCCGCTGGCGCAACAGGCTGCCGAAGCGATGCGCAGTGCGGCCAGCGCACGGCT
GGTCAAGCTGCGCACCGAGCGGATTCGCGAAGTTGCCCCGCTGCCTTTCGATCTGGGCACCCTGCAGGAAATCTGCTCGA
AAAAGCTCGGGCTTGGGGCGCAGGAAACCCTTGATATAGCTCAGTCGCTGTATGAAACACACAAGGTCATCACGTACCCG
CGCAGCGATTGCGGTTACCTGCCGAACAGTCAGCATGGCGAAGCCGCGGGTATCATCGCAGTGCTGTGCAAGGCAGACCC
GACGCTGGCCAGCCTGTCGCCACACCTTGACCCGCAACGCCGATCGCGGGCCTGGAATGACGGCAAAGTGACTGCCCACC
ACGGCATCATTCCCACTGCTGCCGTGCGCGGCGTCGAGCGTCTGACCGGCAAGCCGCGAGCGGTGTACACCCTGATCAGA
GCGCGCTATCTGGCGCAGTTCCTGCCCAGCCACGAATATGACCGTACCCAGGCCGACTTCGACTGCGCAGGCCATGCGCT
GCGGGCGGTCGGCAAACAAATTGTCGAGCCAGGCTGGAAACGTGCCATGCCCGAGGCACTGGCACCGGCCAAGGGTCGCG
AAGCACCTGCGCCGCAGCCGTTGCCGGCATTGTTTCAAGGCTGCGAGTGCACCGTCGCTGAGGTCATCCTCAAGGACCTC
TGGACCCAACCGCCCAAACCTTTTACCGAAGGCGATCTGATCAAGGCCATGAAAAACGTCGCCAAACTGGTGGAAGACCC
GCTGCTCAAGCAAAAGCTCAAGGACACGACCGGCATCGGTACCGAAGCCACCCGCGCCGGGATCATTCAGGGCCTGCTGG
ACCGTGGCTATCTGACCAAACAGGGCAAGGCCCTGTGCGCAGCGCCCGCAGCATTCAGCCTGATCGACGCCGTCCCCCGC
GCCATCGCCGACCCAGGCACTACAGCGATCTGGGAACAGGCACTCGACATGGTACAGAATGGCGAAATGAGCCTTGAAGA
GTTCGTCGCCAAACAGGCAGCGTGGATGAGCAAACAGGTCAGCCGCTGCGTCGGCATGCGCATGAGCATCAGCGGCCCGG
CCAGCCCGGCAGGCGTTGCGCCGCCCTGGAAGAAAAAACGCAAAATCGCCAAGCGCAGCACTGCTACGTCCTCTGCTGCA
ACGGGCACTGCGACGAAAAAACCGCGACGTACAGCCAAACCGGCGGCCAGATGA

Protein sequence :
MRLFLCEKPSQAKDIAAALGATRRGDGCWVGANATVTWCIGHLLETAPPDAYDARYKRWVLEDLPIIPEKWKMLVKPRTA
SQFKAVKRLLGEAQELVIATDADREGEMIARELVEHCRYRGPIQRLWLSALDDASIRKALAALKPGADTFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIANFVPAPYWAIDVRLLHDNQAFIAQWRAPSDAC
DDQDRCLNQPLAQQAAEAMRSAASARLVKLRTERIREVAPLPFDLGTLQEICSKKLGLGAQETLDIAQSLYETHKVITYP
RSDCGYLPNSQHGEAAGIIAVLCKADPTLASLSPHLDPQRRSRAWNDGKVTAHHGIIPTAAVRGVERLTGKPRAVYTLIR
ARYLAQFLPSHEYDRTQADFDCAGHALRAVGKQIVEPGWKRAMPEALAPAKGREAPAPQPLPALFQGCECTVAEVILKDL
WTQPPKPFTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRAGIIQGLLDRGYLTKQGKALCAAPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQNGEMSLEEFVAKQAAWMSKQVSRCVGMRMSISGPASPAGVAPPWKKKRKIAKRSTATSSAA
TGTATKKPRRTAKPAAR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topB AAN62194.1 putative DNA topoisomerase III Not tested PAGI-2(C) Protein 0.0 63
topB AAN62317.1 putative DNA topoisomerase III Not tested PAGI-3(SG) Protein 2e-179 62
pph56 CAI36097.1 Topoisomerase IA Not tested PPHGI-1 Protein 0.0 61
topB AAF14809.1 topoisomerase B Not tested SPI-7 Protein 8e-141 49
topB NP_458624.1 topoisomerase B Not tested SPI-7 Protein 1e-140 49
topB NP_807836.1 DNA topoisomerase III Not tested SPI-7 Protein 1e-140 49
topB1 YP_005176228.1 DNA topoisomerase III Not tested ICEPmu1 Protein 3e-140 49
PMI2630 YP_002152332.1 DNA topoisomerase III Not tested Not named Protein 3e-140 48
topB AAW83109.1 TopB Not tested GGI Protein 1e-107 42