Gene Information

Name : lysS (MSC_0069)
Accession : NP_975080.1
Strain : Mycoplasma mycoides PG1
Genome accession: NC_005364
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 78406 - 79908 bp
Length : 1503 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTTAGATAATAGAAAATTTAGTGAACAAGAATTAGTCAGAAGAAATAAATATAAAACTTTAGTTGAACAAAACAAAGA
TCCTTATAAAGTTACTAATTGAAAAAGAAACACTACATTATTAAAGTTAAATGAAAAATATAAAGATTATAGTAAAGAAG
ATTTATTAAATTTAAATCAAGAATTAGTAGTAGTTGCTGGAAGAATTAAATTATATAGAGAGGCTGGTAAAAAAGCTGCT
TTTGTTAATATTGATGACCAAGATTCAAGTATTCAATTATATGTAAGATTAGATGAAATAGGTGATCAATCTTTTGAAGA
TTTTAGAAACTTCGATTTAGGAGATATTATTGGGGTTAAAGGAATCATGATGAGAACTGATCATGGCGAACTTTCAATTA
GATGTAAAGAAGTTGTTTTATTATCTAAAGCTTTAAGACCACTACCTGATAAACACGCTGGAATTCAAGATATAGAAGAA
AAATATCGTAGAAGATATGTTGATTTAATTGTAAATCATGACGTTAGAAAAACATTTCAAGCTCGAACTAAAATAATAAG
AACTTTACAAAACTTTTTAGATAATAAAGGTTATATGGAAGTTGAAACTCCGATTTTACACTCATTAAAAGGAGGAGCTT
CTGCTAAGCCTTTTATTACTCACTATAATGTTTTAAATACTGATGTTTATTTAAGAATAGCTACAGAACTACATTTAAAA
CGTTTAATTGTTGGTGGTTTTGAAGGGGTTTATGAAATAGGTCGTATTTTTAGAAATGAAGGAATGAGTACTCGCCACAA
TCCTGAATTTACAAGTATTGAATTATATGTTGCTTATGAAGATATGTTCTTTTTAATGAATTTAACAGAAGAAATTTTTA
GAGTATGTAATGCTGCAGTTAATTCAAATTCTATAATTGAATATAATAATGTAAAAATCGATCTATCAAAACCATTTAAA
AGATTACATATGGTTGATGGTATTAAACAAGTAACAGGTGTTGATTTTTGAAAAGAAATGACTGTACAACAAGCTTTAGA
ATTGGCTAAAAAACATAAAGTTCATGTTGAAAAACATCAAGAATCAGTTGGACATATTATTAATTTATTTTATGAAGAAT
TTGTTGAATCAACTATTGTTGAACCAACTTTTGTTTATGGTCATCCAAAAGAAATTTCTCCACTAGCAAAATCTAATCCA
AGCGATCCAAGATTTACTGATAGATTTGAATTGTTTATTCTTGGAAGAGAATATGCTAATGCATTTAGTGAGTTAAACGA
TCCAATTGATCAATATGAAAGATTTAAAGCTCAAATTGAAGAAGAATCTAAAGGAAATGATGAAGCCAATGATATGGATA
TTGACTTTATTGAAGCCTTAGAACACGCAATGCCACCAACTGCTGGAATTGGTATAGGAATTGATAGATTAGTAATGCTT
TTAACTAATTCAGAATCTATTAAAGATGTATTATTATTTCCACAAATGAAGCCAAGAGAATAA

Protein sequence :
MLDNRKFSEQELVRRNKYKTLVEQNKDPYKVTNWKRNTTLLKLNEKYKDYSKEDLLNLNQELVVVAGRIKLYREAGKKAA
FVNIDDQDSSIQLYVRLDEIGDQSFEDFRNFDLGDIIGVKGIMMRTDHGELSIRCKEVVLLSKALRPLPDKHAGIQDIEE
KYRRRYVDLIVNHDVRKTFQARTKIIRTLQNFLDNKGYMEVETPILHSLKGGASAKPFITHYNVLNTDVYLRIATELHLK
RLIVGGFEGVYEIGRIFRNEGMSTRHNPEFTSIELYVAYEDMFFLMNLTEEIFRVCNAAVNSNSIIEYNNVKIDLSKPFK
RLHMVDGIKQVTGVDFWKEMTVQQALELAKKHKVHVEKHQESVGHIINLFYEEFVESTIVEPTFVYGHPKEISPLAKSNP
SDPRFTDRFELFILGREYANAFSELNDPIDQYERFKAQIEEESKGNDEANDMDIDFIEALEHAMPPTAGIGIGIDRLVML
LTNSESIKDVLLFPQMKPRE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 4e-100 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS NP_975080.1 lysyl-tRNA synthetase VFG1668 Protein 2e-100 46