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Name : lysS (LJ0287) Accession : NP_964304.1 Strain : Lactobacillus johnsonii NCC 533 Genome accession: NC_005362 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 325951 - 327492 bp Length : 1542 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : GTGGCAAAGAATGAAATGAACGACCAACTAATCGCTCGTCGTGAAAAAATGGACGAAATGCGTGAAAACGGTATTGAACC TTTTGGCGTAAGAAAATTTGATCGTCAAGACTTAGCTCGTACTTTGAATGAAAAGTATAGTAATGAAGATAAAGATGAAT TAAATGCTGATATGCCTATGACTAAGATTGCAGGTCGTATGCTTGCAAAACGTGGTAAGGGTAAAGTTGGATTTGCAGAT CTTTATGACCGTACTGGTAAAATTCAAATTTACGTACGTAAAGATATCGTTGGTGAAGATAACTACAAGATTTTTAAGAA GTCTGATATTGGTGACTTTTTAGGTATTGATGGTGAAGTAATGAAGACCGATACTGGTGAGTTGACTATTCGTGCTACTC ACATTACTTTCTTGTCTAAGGCTCTTCGTCCATTGCCTAATAAATGGGATGGTTTAAAGGATGTTGAACAAATTTATCGT CAGCGCTACCTTGACTTAATTACAAATCATGATAGTTACATGCGTTTTGTTCACCGTACTCAAATTATTCAAGCTATTCG TGACTATTTGAATAATAGAGATTTCTTAGAAGTTGAAACTCCAGTTCTCCACAATATTCCTGGTGGTGCTGAAGCTCGTC CATTTATTACTCACCACAATGCTTTGGATATTGATCTTTACATGAGAATTGCTTTGGAACTTCCATTGAAGCGTTTAATT GTTGGTGGTATGGAACGTGTTTATGAAATCGGCCGTGTATTTAGAAATGAAGGTCTTGATACTAAGCACAACCCAGAATT TACTGAACTTGAAACTTATGCAGCTTACTGGGACTTCCATGATGTAATGGATGAGGCAGAAGGAATTATTAGAGCAGCTG CTAAAGTTGTTTCACCAGATGGTAAGATTACTTACCAAGGAACTGATATTGACTTAGGTAAGCCATTCCGCCGTGTTCAC ATGGTTGATTTAATCAAGGAAAAGACTGGTGTTGACTTCTGGAAGCCAATGACTATTGAAGAAGCAAGAAAAGTTGCTGA TGAACACAACGTTCACTATGAAAGCTACTGGAAGGTTGGCCACATTATTAATGCCTTCTTTGAAGAATTCGGTGAAGGAT CAATTGTTCAACCTACTTTTGTTTATGGACACCCAGTAGAAGTATCACCACTTGCTAAGAAGAATGCTGATGATCCAAGA TTTACTGATCGTTTTGAAATCTTCATTATGGGTGCAGAATATGGTAATGCATTCTCAGAATTAAATGATCCAGATGATCA ACGTCATCGTTTCGAAGACCAAATGGCTGAAAGAGAAGCTGGTAACGATGAAGCAGATATGATTGATGAGGATTACCTTC GTGCTATGGAATTCGGTATGCCTCCTACAGGTGGTCTAGGTATTGGTATTGATCGTTTGGTAATGCTTTTAACTGATGCT CCTGCTATTCGTGATGTCTTACTCTTCCCAACAATGCGCCCTGAAAAGGTTGAAAGTGTTGAAGCAGAAGTTCAAGAAGA TTTGAAGAAAAAGAACAAATAG Protein sequence : MAKNEMNDQLIARREKMDEMRENGIEPFGVRKFDRQDLARTLNEKYSNEDKDELNADMPMTKIAGRMLAKRGKGKVGFAD LYDRTGKIQIYVRKDIVGEDNYKIFKKSDIGDFLGIDGEVMKTDTGELTIRATHITFLSKALRPLPNKWDGLKDVEQIYR QRYLDLITNHDSYMRFVHRTQIIQAIRDYLNNRDFLEVETPVLHNIPGGAEARPFITHHNALDIDLYMRIALELPLKRLI VGGMERVYEIGRVFRNEGLDTKHNPEFTELETYAAYWDFHDVMDEAEGIIRAAAKVVSPDGKITYQGTDIDLGKPFRRVH MVDLIKEKTGVDFWKPMTIEEARKVADEHNVHYESYWKVGHIINAFFEEFGEGSIVQPTFVYGHPVEVSPLAKKNADDPR FTDRFEIFIMGAEYGNAFSELNDPDDQRHRFEDQMAEREAGNDEADMIDEDYLRAMEFGMPPTGGLGIGIDRLVMLLTDA PAIRDVLLFPTMRPEKVESVEAEVQEDLKKKNK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 7e-109 | 47 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | NP_964304.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 4e-109 | 47 |
