Gene Information

Name : pykF (PAM_287)
Accession : NP_950539.1
Strain : Onion yellows phytoplasma onion yellows
Genome accession: NC_005303
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 333629 - 334969 bp
Length : 1341 bp
Strand : +
Note : identified by sequence similarity; putative

DNA sequence :
ATGAACAAAACTAAAATAATTTGTACCTTAGGACCTGCTTCTTATGATAAAAACATTTTACAAGCCTTAATTCAAACAGG
TTTGAATGTAGCAAGATTTAATTTTTCTCACGCTCAATACGAACAAACTAAACTTTTAATGAAAACCATTAAAACTATTA
GCGATAAATTAGACAAAAACACAGGACTTATGTTAGATACTAAAGGACCTGAAATTAGAACTCACGAATTTGATGGTGTA
GTAACAATTCAAAAAGATTCTGAAGTAAAAATATCTATGACAGAAGTTTTAGGTAATGCTAAACTTTTTTCAGTTAGTTA
TTCTAATTTATACAATGAATTAAAAGTTGGCGATATGGTTAATATTGATGATGGATACTTATCTTTGGAAGTTGTAGGCA
AAGACGAAGCCAAACAACAATTAGTTACTAAAGCCAAAAATACTCACTCCATCAAATCTCGTAGAGGTGTAAATGTTCCT
AAAGTTAATTTAGAAATGGATTTTATTTCTCCTAAAGATTATCAAGATATTGTTTTTGCTGCCCAACAAGATTTTGATTA
TATTGCTGCTTCATTTGTTAGAAGAGCTCAAGATGTCAAAGATATTAGAAAAATTTTACAAGAACAAGGAAATTCCAACA
TCCAAATTATTTCTAAAATAGAAAATCAAGAAGGTGTTGATAATTTAGAAGAAATCATTCAAGAATCAGACGGTATTATG
GTTGCAAGAGGTGATTTAGGAATAGAAGTTGATGGTGAATTAGTACCACTTTACCAAACGCGTATGATTACTAAATGTTT
AGAATACGGCAAACCAGTTGTAGTAGCTACTCAAATGTTAGAATCTATGCAAAGAAATCCGCGCCCAACCAAAGCTGAAA
CTTCTGATGTCTTTAATGCAGTTAGAGAAGGCACCACCTTTACTATGCTATCGGGCGAAAGTGCTTCAGGTGAATATCCT
GTAGAAGCTGTAACTTATATGAAAAAAATTAATTATCAAGCTGAAAAAGTAGTTAATTATCAAGCTTTATCACAAGTATA
CCAACCTAAAAACTCCAAAGAAAACCTTTTATTAAGTGCTGTGGAATTGGCTTTACGCACCGATGTTAAAGCAATTGTTG
TCTATGACTTAAAAGATGCTTATAATGTTTCTAAATTTCATCCATCCGTTCCTGTTTTGGCTTTAGTTAAAACTGAACAA
GAAGCTCGTCGTTTAGTACTAAATTTTGGGGTTTGTCCTTTTGTTTCTGAAACCAAATTACAAACTAAATTGGAAGAACT
TACCCAAAATCAAAGTAGTAATTGTCTTGTTGTTAAAGATGGGATGCTTTATTTTAAATAA

Protein sequence :
MNKTKIICTLGPASYDKNILQALIQTGLNVARFNFSHAQYEQTKLLMKTIKTISDKLDKNTGLMLDTKGPEIRTHEFDGV
VTIQKDSEVKISMTEVLGNAKLFSVSYSNLYNELKVGDMVNIDDGYLSLEVVGKDEAKQQLVTKAKNTHSIKSRRGVNVP
KVNLEMDFISPKDYQDIVFAAQQDFDYIAASFVRRAQDVKDIRKILQEQGNSNIQIISKIENQEGVDNLEEIIQESDGIM
VARGDLGIEVDGELVPLYQTRMITKCLEYGKPVVVATQMLESMQRNPRPTKAETSDVFNAVREGTTFTMLSGESASGEYP
VEAVTYMKKINYQAEKVVNYQALSQVYQPKNSKENLLLSAVELALRTDVKAIVVYDLKDAYNVSKFHPSVPVLALVKTEQ
EARRLVLNFGVCPFVSETKLQTKLEELTQNQSSNCLVVKDGMLYFK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 6e-70 42
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 6e-70 42
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 5e-70 42
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-69 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF NP_950539.1 pyruvate kinase VFG0479 Protein 3e-70 42