Gene Information

Name : lysS (plu3548)
Accession : NP_930765.1
Strain : Photorhabdus luminescens TTO1
Genome accession: NC_005126
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 4181518 - 4183032 bp
Length : 1515 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCACAACAACAACAGGGTGCGGAACAGGCCCCCGATTTAAATAATGAGCTGCAAACTCGCCGCGAAAAACTTGCCGC
TTTACGGGAGAATGGAATTGCATTCCCGAATGATTTCCGTCGTGAAAATATTTCAGAAGATTTGCACGCGAAATACGACG
ACAAAACTCAGGAAGAGCTGGAAGCACTGAATATTGACGTGACGGTTGGCGGTCGTATGATGACGCGTCGCATTATGGGT
AAGGCTTCTTTCGTAACGCTACAGGATATGGGCGGTCGTATTCAGTTATATGTTGCCCGTGACGATCTGCCGGAAGGTAT
TTATAACGAACAGTTTAAAAAATGGGATCTGGGAGACATTCTGGGGGCTCGTGGCAAACTGTTTAAAACCAAAACCGGTG
AACTTTCCATTCACTGTACTGAACTGCGCCTGCTGACAAAAGCGCTGCGTCCATTGCCGGATAAATTCCACGGCTTGGCT
GATCAGGAAACTCGCTATCGCCAGCGTTATCTGGATCTGATCGCCAACGAAGAATCGCGCAAAACTTTCCAAATTCGCTC
TCAAGTCTTATTGGCCCTGCGTAGCTTTATGGTCAGTAAAGGCTTTATGGAAGTGGAAACACCCATGATGCAGGTAATCC
CTGGTGGTGCGGCGGCTCGTCCATTTATTACTCACCATAATGCGCTGGATATTGATATGTATCTGCGTATTGCGCCGGAA
CTTTACCTGAAACGTCTGGTTGTTGGTGGTTTTGAACGTGTCTTTGAAATTAACCGCAACTTCCGTAACGAAGGCGTATC
TCCGCGCCATAACCCTGAGTTCACCATGATGGAACTCTACATGGCCTATGCGGATTACAAAGATCTGATTGTCCTGATAG
AAGAGCTATTCCGTACACTGACGCAGAATATTTTGGGGAATACTCTGGTTAAATATGGTGAGCAGGAATTCGATTTCGGT
AAACCATTTGCCCAAATGACCATGAAAGAGGCCATTTGCAAATATCGTCCTGAAACCAACATAGCTGATTTGGATGATAT
GGATAAGGTGGTGGCTATCGCTGAATCTCTTGGTATTGAAGTGGAAAAAGGTTGGGGCCTTGGTCGTGTTCAGTGTGAAA
TTTTTGAAGAAACGGCGGAAAGCCACCTGATTCAACCGACATTTATCACTGAATATCCGGCAGAAGTTTCTCCGCTAGCG
CGTCGTAATGATGCAAATCCGTTTATCACTGACCGTTTTGAATTCTTTATTGGTGGACGTGAGATTGGTAATGGCTTCTC
GGAATTGAATGATGCCGAAGATCAGGCGGAACGTTTTGCTGAGCAGGTTCGTCAGAAAGACGAAGGTGATGACGAAGCCA
TGTTCTACGATGAAGATTATGTAACGGCTTTGGAACACGGTATGCCGCCAACCGCCGGTTTGGGCATTGGTATTGACCGT
ATGATAATGTTGTTGACAGATAGCCATACCATCCGTGATGTTATTCTGTTCCCGGCAATGCGCCCGCAGAAATAA

Protein sequence :
MSQQQQGAEQAPDLNNELQTRREKLAALRENGIAFPNDFRRENISEDLHAKYDDKTQEELEALNIDVTVGGRMMTRRIMG
KASFVTLQDMGGRIQLYVARDDLPEGIYNEQFKKWDLGDILGARGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGLA
DQETRYRQRYLDLIANEESRKTFQIRSQVLLALRSFMVSKGFMEVETPMMQVIPGGAAARPFITHHNALDIDMYLRIAPE
LYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLIEELFRTLTQNILGNTLVKYGEQEFDFG
KPFAQMTMKEAICKYRPETNIADLDDMDKVVAIAESLGIEVEKGWGLGRVQCEIFEETAESHLIQPTFITEYPAEVSPLA
RRNDANPFITDRFEFFIGGREIGNGFSELNDAEDQAERFAEQVRQKDEGDDEAMFYDEDYVTALEHGMPPTAGLGIGIDR
MIMLLTDSHTIRDVILFPAMRPQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-169 67
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-91 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS NP_930765.1 lysyl-tRNA synthetase VFG1668 Protein 8e-170 67