Gene Information

Name : entE (plu2729)
Accession : NP_929963.1
Strain : Photorhabdus luminescens TTO1
Genome accession: NC_005126
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 3235684 - 3237303 bp
Length : 1620 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGTCTATTGAATTTACACCCTGGCCGGTTGAGTTAGCAGAGCGTTATCGTGAAAAGGGTTATTGGCTGGGTAAACCCTT
AACTGACATACTTTATAAACAATGTCAGCGACAACCTGAAGCAATAGCTATTATCTGTGGTAACCGTAAATTTAGTTATC
ATCAACTTGATCAACAATCATCTGCATTAGCCTCAAATTTAACTTTAGCGGGAATAAAGAGAGGTGATACTGCTATCGTA
CAATTGCCAAATATAGCTGAATTTTATATTACATTTTTTGCGCTACTGAAAATGGGGGTAGTACCTATTAATGCATTGTT
TAGCCATAATAAATTAGAGTTACTTGCTTATGCACAACAAATTGATCCAAGTCTGATTGTACTTTCGTCAGAGCATTCAC
TTTTACAGAATAATGATTTCCTGGAGCAATTAAAAATACATTGCCCACGGTTAAGGAATATCGTCGTTGATGGTCGAAAA
ATACAATATACCAATGAATTAAAATCGTGGTTAGAGTCAACAAATTTACCTTTGAATTTTGAGGCGACACGTTCAGATGA
AGTCGCTTTTTTTCAACTTTCAGGCGGGAGTACAGGGACACCTAAATTAATTCCAAGGACGCATGACGATTACTATTATA
GTGTGAGGCGCAGTGTCGAAATTTGTGAGTTGACACAACAGACCCGTTATCTTTGTGCTTTGCCGGCGCAACATAATTAT
CCTTTGAGTTCTCCGGGGGCGCTTGGCGTATTTTATGCTGGTGGTTGTCTTATATTAGCAACAGATCCTGGGCCTACGAC
CTGCTTTCCACTTATACGTGAACATAAAGTAACAATGACCTCATTGGTGCCATCGGCTGTTGCGTTATGGATTCAGACGA
TGAGGAAATACCAATATTCACTAGAAAGTCTTCGGCTATTACAAGTCGGTGGAGCAAAGTTAAGTGAAAGTGTGGCGCGA
GTGATACCGAAACTTTTTTCATGTAAGTTGCAACAGGTTTTTGGTATGGCCGAAGGGTTGGTATGTTATACCCGGCTTAA
TGACGATGATGAGCGTATATTTACCACTCAGGGATGCCCAATGAGTGGAGATGATGAGGTACGTATCGTTGATGATAACG
GCAATATACAAGCGCCAGGTTCAGCGGGGATGTTAATCATTCGTGGGCCATATACATTCCGGGGATATTATAAAAGCCCA
GCGCACAATGCGAAGGTATTCGACAAAGAAGGTTTTTATTATACCGGTGATTTAGCCTATATGACGAAAGAGGGTTATAT
ATGTATTGTCGGGCGTGAGAAAGATCAAATTAATCGCGGTGGTGAAAAAATTGCTTCGGAAGAAATTGAATCTTTGCTAC
TCAAACATGAAGATGTTATCCATAGCGCTTTGGTTTCGATGCCTGATCCTTTGATGGGAGAAAAAAGCTGTGCTTTTATT
GTGGTGGCTGATCCGAATATAAACACGTTGCAACTGCGGAAATATTTAAGGGGAATGGGGATTGCTGAATATAAATTGCC
TGATCGTTTCGAGATTATTGATGTTATGCCGGTTACACCCGTCGGAAAGATTGATAAATTATTATTAAGAAAACTCATTC
GAGACAAATTAGAAAATTAA

Protein sequence :
MSIEFTPWPVELAERYREKGYWLGKPLTDILYKQCQRQPEAIAIICGNRKFSYHQLDQQSSALASNLTLAGIKRGDTAIV
QLPNIAEFYITFFALLKMGVVPINALFSHNKLELLAYAQQIDPSLIVLSSEHSLLQNNDFLEQLKIHCPRLRNIVVDGRK
IQYTNELKSWLESTNLPLNFEATRSDEVAFFQLSGGSTGTPKLIPRTHDDYYYSVRRSVEICELTQQTRYLCALPAQHNY
PLSSPGALGVFYAGGCLILATDPGPTTCFPLIREHKVTMTSLVPSAVALWIQTMRKYQYSLESLRLLQVGGAKLSESVAR
VIPKLFSCKLQQVFGMAEGLVCYTRLNDDDERIFTTQGCPMSGDDEVRIVDDNGNIQAPGSAGMLIIRGPYTFRGYYKSP
AHNAKVFDKEGFYYTGDLAYMTKEGYICIVGREKDQINRGGEKIASEEIESLLLKHEDVIHSALVSMPDPLMGEKSCAFI
VVADPNINTLQLRKYLRGMGIAEYKLPDRFEIIDVMPVTPVGKIDKLLLRKLIRDKLEN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-89 41
ybtE CAA21394.1 - Virulence HPI Protein 3e-89 41
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-89 41
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-89 41
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 3e-89 41
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-89 41
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 5e-88 41
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 7e-88 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE NP_929963.1 enterobactin synthase subunit E VFG0932 Protein 2e-147 58
entE NP_929963.1 enterobactin synthase subunit E VFG0168 Protein 4e-105 42