Gene Information

Name : ddl (CV_2472)
Accession : NP_902142.1
Strain : Chromobacterium violaceum ATCC 12472
Genome accession: NC_005085
Putative virulence/resistance : Resistance
Product : D-alanyl-alanine synthetase A
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 2659229 - 2660329 bp
Length : 1101 bp
Strand : -
Note : D-alanine--D-alanine ligase; DdlA; DdlB; cytoplasmic; catalyzes the formation of D-alanyl-D-alanine from two D-alanines in peptidoglycan synthesis; there are two forms of this enzyme in Escherichia coli

DNA sequence :
ATGGGCAAAATCCGGGTGGGTCTGATTTTCGGCGGACAGTCGTCCGAGCACGAGGTTTCCTTGCAATCTGCCAGGAATAT
CCTGCAAGCGATAGACGGCGAGCGTTTCGAAGTTTCGCTGATCGGTGTGGACAAGCAAGGCCGCTGGCATGCCAGCCAGG
CGTCCAATTTTCTGCTGAACGCCGACGATCCCGGCCGCATCGCCTTGCGCGAGTCCGGAGAAAACCTGGCCCTGGTGCCG
GGCGAATGTTCGGGCCAGCTGCAGACGGCGGCGAATGCGCATCCGCTGGCGCAGATCGACGTGGCCTTCCCCATCGTCCA
CGGCACGCTGGGCGAGGACGGCTCGCTGCAAGGCCTGCTGCGAATGGCCAACATCCCCTTCGTCGGCGCGGGCGTGCTGG
GTTCGGCGGTTTGCATGGACAAGGACGTGGCCAAGCGCCTGTTGCGCGATGCGGGCCTCAAAGTCGCTCCTTTCGTCAGC
CTGACTCGATCCAAGGCCGCCGGCGCCGATCTCTCCGCCATCGTCGAGCAGCTGGGATTGCCGCTGTTCGTCAAGCCGGC
CAATCAGGGCTCATCCGTAGGGGTGAGCAAAGTGAAGCGGGAGGCGGATTTGCGCGCGGCTCTGGACGAAGCTTTCCGTT
ACGATCACAAGGTCCTGGTGGAGCAGGCGGTGATAGGCCGCGAAATCGAATGCGCGGTGCTGGGCAACGAGCGCCCGCGG
GCCAGCGGCTGCGGCGAGATCGTGTTGAGCGATGAGTTCTACGCCTACGACACCAAGTATCTGAATGAGGACGGCGCCCG
CGTCGCAGTGCCGGCCGACATCCCGGACGAGGCCTGCCAGCGCATCCGCGGCATCGCCATTGAGGCTTTCCAGGCGCTGG
AGTGCAGCGGCATGGCGCGCGTGGATGTGTTCCTGACGCCGGATGGCGAGGTGGTGATCAATGAACTGAACACGCTGCCG
GGTTTCACCAATATCAGCATGTACCCCAAGCTGTGGCAGGCGGCTGGGATGTCGTATCGGGAATTGATCACCGCGCTGAT
AGAGTTGGCGCTGGAGAAAGGCCGGATGGATGAAGCGTTGAGCCGCAGTTGCCAGTACTAG

Protein sequence :
MGKIRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLNADDPGRIALRESGENLALVP
GECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLLRMANIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVS
LTRSKAAGADLSAIVEQLGLPLFVKPANQGSSVGVSKVKREADLRAALDEAFRYDHKVLVEQAVIGREIECAVLGNERPR
ASGCGEIVLSDEFYAYDTKYLNEDGARVAVPADIPDEACQRIRGIAIEAFQALECSGMARVDVFLTPDGEVVINELNTLP
GFTNISMYPKLWQAAGMSYRELITALIELALEKGRMDEALSRSCQY

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 4e-49 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ddl NP_902142.1 D-alanyl-alanine synthetase A AY145441.gene.p01 Protein 4e-50 41
ddl NP_902142.1 D-alanyl-alanine synthetase A U35369.1.gene6.p01 Protein 2e-49 41
ddl NP_902142.1 D-alanyl-alanine synthetase A AE016830.1.gene2250. Protein 2e-49 41
ddl NP_902142.1 D-alanyl-alanine synthetase A U00456.gene.p01 Protein 2e-49 41