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Name : lysS (CV_1060) Accession : NP_900730.1 Strain : Chromobacterium violaceum ATCC 12472 Genome accession: NC_005085 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 1106914 - 1108422 bp Length : 1509 bp Strand : - Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCTGATCACGAACAAGCACAGGCGCAAAGCCAAGACGAAAACCAGATCATGGCGGAACGCCGCCAGAAACTGCAGGC GATCCGCGAAAAAGGCATCGCCTACCCCAACGACTTCAAACGCAGCCACTTCGCCAAACCGCTGCAGGACGACCACGCCG CCAAAGAGGCAGAGGCGCTGGAAGCCGAGAAAATCGAAGTCGCCGTCGCCGGCCGCATGATGCTTAAACGTGTGATGGGC AAAGCCAGCTTCGCCACCCTGCAGGACGTCAGCGGCCGCATCCAGGCCTTCATCTCCCGCGACAACGTGGGCGAAGACGT CTACGCCGACTTCAAGCGCTGGGACCTGGGCGACATCGTCGCCGTCAAGGGCATGCTGTTCAAGACCAAGACCGGCGAAC TGACGGTGCAGGCGACCGAAGTGCGCATGCTGTCCAAGAACATCCGTCCGCTGCCGGAGAAATTCCACGGCATCGCCGAC CAGGAAACAAAGTACCGCCAGCGCTACGCCGACCTGATCATGAGCGAGGAAAGCCGCGAAACCTTCATCAAGCGCTCCAA GATCGTGCAGAAGGTGCGCGACGTGATGGTGGGCGAAGGCTACCTGGAAGTGGAAACCCCGATGATGCACCCGATCCCGG GCGGCGCGTCGGCCAAACCCTTCGTCACCCACCACAACGCGCTGGACATGCCGCTCTACCTGCGCATCGCCCCGGAGCTG TACCTGAAGCGCCTGGTGGTCGGCGGCCTGGAACGCGTGTTCGAGATCAACCGCAACTTCCGCAACGAGGGGATGAGCAC GCGCCACAACCCCGAGTTCACCATGATCGAGTTCTACGAAGCATATAGCGACTACCAGCGCATGATGGAGATGACCGAAA CCATCATCCGCGAGTGCGCGCTGGTTGCCTGCGGCTCCACCACCGTGACCTACCAAGGCAAGGAAGTGGACCTGGGCAAG CCGTTCGACCGCTTCACCATCGTGCAGGCGATCAAGCACTACAACCCGCAATACACCGACGCGCAACTGTCCGACGCCGC CTGGGTGGCCAGCGAGATCAAGCGCCTGGGCGGCAAGCTGCCCCCGGCCCCGGGCCTGGGCAGCCTGCAGCTGGCGCTGT TCGAGGAATGCGCCGAATCGCTGCTGTGGAACCCGACCTTCATCATCGACTACCCGGTGGAAGTGTCCCCGCTGGCGCGC GGCTCCGACACCCAGCCGGGCCTGACCGAGCGTTTCGAACTGTTCATCGTCGGCCGCGAGCACGCCAACGGCTACTCGGA GTTGAACGATCCGGAAGACCAGGCCGCGCGCTTCCAGGCGCAAGTCGCCCAGAAGGACGCCGGCGACGACGAGGCGATGC ACTACGACGCCGACTACATCCGCGCGATGGAATACGGCCTGCCGCCGACCGGCGGTTGCGGCATCGGCATCGACCGCTTG GTGATGTTGTTGACGGATGCGCCTTCTATCCGGGATGTGATCTTGTTCCCGCATATGCGTCCGGAGTAA Protein sequence : MSDHEQAQAQSQDENQIMAERRQKLQAIREKGIAYPNDFKRSHFAKPLQDDHAAKEAEALEAEKIEVAVAGRMMLKRVMG KASFATLQDVSGRIQAFISRDNVGEDVYADFKRWDLGDIVAVKGMLFKTKTGELTVQATEVRMLSKNIRPLPEKFHGIAD QETKYRQRYADLIMSEESRETFIKRSKIVQKVRDVMVGEGYLEVETPMMHPIPGGASAKPFVTHHNALDMPLYLRIAPEL YLKRLVVGGLERVFEINRNFRNEGMSTRHNPEFTMIEFYEAYSDYQRMMEMTETIIRECALVACGSTTVTYQGKEVDLGK PFDRFTIVQAIKHYNPQYTDAQLSDAAWVASEIKRLGGKLPPAPGLGSLQLALFEECAESLLWNPTFIIDYPVEVSPLAR GSDTQPGLTERFELFIVGREHANGYSELNDPEDQAARFQAQVAQKDAGDDEAMHYDADYIRAMEYGLPPTGGCGIGIDRL VMLLTDAPSIRDVILFPHMRPE |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 7e-118 | 51 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 1e-78 | 43 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | NP_900730.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 4e-118 | 51 |
