Gene Information

Name : sepA (CP0070)
Accession : NP_858203.1
Strain :
Genome accession: NC_004851
Putative virulence/resistance : Virulence
Product : extracellular serine protease SepA
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG3468
EC number : -
Position : 52745 - 56839 bp
Length : 4095 bp
Strand : -
Note : -

DNA sequence :
ATGAACAAAATATATTATCTTAAGTATTGCCATATAACCAAAAGCCTGATTGCTGTCTCCGAACTGGCCCGCAGGGTTAC
CTGCAAAAGCCATCGCAGACTTTCACGTCGGGTTATTCTTACGTCTGTTGCAGCTTTATCACTTTCCTCGGCATGGCCAG
CTCTGTCAGCAACGGTCAGTGCAGAGATCCCTTATCAGATATTCCGCGACTTTGCCGAGAATAAAGGTCAGTTTACGCCG
GGGACCACGAATATTTCCATTTACGACAAGCAGGGCAATCTGGTCGGTAAACTTGATAAAGCCCCGATGGCTGATTTCAG
TAGTGCCACTATTACTACCGGTAGCCTGCCTCCGGGAGACCATACACTTTACTCCCCTCAGTATGTGGTTACCGCCAAAC
ATGTCAGCGGTTCTGACACGATGAGCTTCGGATACGCCAAAAACACCTATACTGCGGTTGGTACAAATAATAATAGCGGT
CTTGATATTAAAACCCGCCGCCTGAGTAAACTGGTGACAGAGGTGGCGCCGGCAGAAGTGTCTGACATCGGGGCTGTGAG
CGGTGCCTATCAGGCCGGCGGACGCTTTACTGAATTCTATCGCCTTGGGGGGGGGATGCAGTACGTCAAAGACAAAAATG
GTAATCGCACCCAAGTGTATACCAATGGCGGATTTCTTGTTGGGGGAACCGTCAGCGCGCTGAACTCCTATAACAACGGA
CAGATGATTACAGCTCAGACCGGTGACATTTTTAATCCCGCGAATGGACCTCTGGCTAATTATCTGAATATGGGGGACAG
CGGCTCCCCCCTGTTTGCTTATGATTCCCTGCAAAAAAAATGGGTACTGATTGGCGTGCTTTCCTCAGGAACTAACTATG
GAAATAACTGGGTCGTCACCACTCAGGATTTCCTTGGTCAGCAACCGCAAAATGATTTTGACAAAACTATAGCCTACACC
TCTGGTGAAGGAGTACTGCAGTGGAAATATGATGCGGCTAATGGCACCGGCACACTGACTCAGGGAAACACGACTTGGGA
TATGCATGGAAAGAAAGGAAATGATCTGAACGCAGGAAAAAACCTCCTGTTCACCGGCAATAATGGCGAGGTCGTTCTGC
AGAATTCCGTTAATCAGGGAGCTGGGTATCTGCAGTTTGCCGGCGATTACAGGGTGTCCGCCCTGAACGGCCAGACATGG
ATGGGGGGCGGGATTATCACCGATAAAGGAACCCACGTTCTGTGGCAGGTGAACGGAGTTGCCGGTGATAACCTGCATAA
AACTGGCGAAGGTACCCTGACAGTAAACGGGACCGGTGTGAATGCAGGCGGACTCAAGGTGGGGGACGGTACCGTTATTC
TCAACCAGCAGGCGGATGCTGACGGAAAGGTACAGGCTTTCAGCTCTGTTGGTATTGCCAGCGGTCGTCCGACAGTTGTG
CTTTCTGACTCACAGCAAGTTAATCCGGATAATATTTCGTGGGGATACCGTGGAGGACGTCTGGAACTGAATGGTAATAA
TCTGACCTTTACCCGTCTTCAGGCGGCTGACTACGGGGCCATCATTACCAATAACAGTGAGAAAAAATCAACGGTAACAC
TCGACCTTCAGACATTAAAAGCCAGTGACATCAATGTACCGGTTAATACTGTCAGTATTTTTGGGGGGAGAGGAGCGCCG
GGAGACCTATATTATGACAGTTCTACCAAGCAATATTTCATCCTGAAAGCGAGTTCATATTCCCCGTTCTTCTCCGATCT
GAACAACAGCAGTGTCTGGCAGAATGTCGGAAAAGATCGTAATAAAGCCATTGATACTGTGAAGCAACAAAAAATTGAGG
CCAGCAGCCAGCCTTATATGTATCACGGCCAACTAAACGGCAATATGGATGTGAATATTCCGCAGCTCTCAGGTAAGGAT
GTACTGGCTCTTGATGGTTCAGTTAACCTGCCAGAAGGTTCGATAACCAAGAAGTCCGGCACGCTGATATTCCAGGGTCA
TCCAGTTATTCATGCTGGAACGACGACTTCTTCCAGCCAGAGCGACTGGGAAACCCGTCAGTTTACGCTGGAAAAACTGA
AACTCGATGCGGCAACATTCCATCTGTCCAGAAACGGCAAGATGCAGGGAGATATTAACGCCACCAATGGAAGTACAGTC
ATTCTGGGAAGTAGCCGTGTCTTTACTGACAGGAGTGACGGAACCGGTAATGCGGTCTTCTCTGTTGAAGGGAGTGCCAC
TGCAACCACAGTTGGTGACCAGAGTGATTACAGCGGAAATGTTACGCTGGAAAATAAATCATCCCTGCAAATCATGGAGA
GATTCACCGGTGGTATTGAGGCTTATGATAGCACCGTGAGTGTGACCTCTCAGAATGCTGTTTTTGACCGTGTTGGCAGC
TTTGTCAACAGCAGCCTGACCCTCGGAAAAGGAGCAAAACTTACGGCTCAGAGCGGTATTTTCAGCACCGGGGCTGTGGA
TGTAAAAGAAAATGCTTCTCTGACCCTGACGGGGATGCCTTCTGCGCAGAAACAGGGGTATTACTCACCCGTAATTTCTA
CAACAGAAGGGATTAACCTCGAAGATAACGCCAGCTTTTCTGTTAAAAATATGGGCTATCTGAGTTCGGATATTCATGCA
GGAACCACTGCGGCAACCATTAATCTGGGAGACAGTGATGCTGATGCAGGGAAGACGGACTCTCCGTTATTCAGCTCCTT
AATGAAGGGATATAACGCTGTTCTGAGAGGCAGTATTACGGGGGCACAGAGTACGGTAAATATGATCAATGCTCTGTGGT
ACTCTGACGGAAAATCAGAGGCCGGTGCACTGAAGGCTAAGGGCTCGCGAATTGAACTGGGGGACGGGAAACACTTTGCC
ACTTTACAAGTAAAAGAGCTTTCTGCAGATAATACCACGTTCCTGATGCATACCAACAACAGCCGGGCTGACCAGTTGAA
TGTCACAGACAAACTGTCAGGCAGTAATAATAGCGTCCTGGTTGACTTTTTAAACAAACCAGCCAGTGAAATGAGCGTGA
CGTTAATTACCGCACCGAAAGGGAGTGATGAGAAAACGTTCACTGCCGGTACGCAGCAGATTGGTTTCAGTAACGTTACG
CCAGTAATCAGCACGGAAAAAACGGATGATGCCACAAAATGGGTGCTGACAGGATATCAGACTACCGCTGATGCCGGAGC
CTCGAAAGCCGCAAAAGACTTTATGGCATCAGGTTATAAGTCCTTCCTTACAGAGGTCAATAACCTGAACAAACGTATGG
GTGACCTGCGGGATACTCAGGGGGATGCCGGTGTCTGGGCACGCATAATGAATGGTACCGGTTCGGCAGATGGTGACTAC
AGCGATAACTACACTCACGTTCAGATTGGTGTCGACAGAAAGCATGAGCTGGACGGTGTGGATTTATTTACGGGGGCATT
GCTGACCTATACGGACAGCAATGCAAGCAGCCACGCATTCAGTGGAAAAAACAAATCCGTGGGTGGCGGTCTGTATGCCT
CTGCACTCTTTAATTCCGGAGCTTATTTTGACCTGATTGGTAAATATCTCCATCATGATAATCAGCACACGGCGAATTTT
GCCTCACTGGGAACAAAAGACTACAGCTCTCATTCCTGGTATGCCGGTGCTGAAGTTGGTTATCGTTACCACCTGACGAA
AGAGTCCTGGGTGGAGCCACAGATAGAGCTGGTTTACGGTTCTGTATCAGGAAAAGCTTTTAGCTGGGAAGACCGGGGAA
TGGCTCTGAGCATGAAAGACAAGGATTATAACCCACTGATTGGCCGTACTGGTGTTGACGTGGGAAGAGCCTTCTCCGGA
GACGACTGGAAAATCACAGCTCGAGCCGGGCTGGGTTATCAGTTCGACCTGCTGGCGAACGGAGAAACGGTTCTGCAGGA
TGCTTCCGGAGAGAAACGTTTCGAAGGTGAAAAAGATAGCAGGATGCTGATGACGGTAGGGATGAATGCGGAAATTAAGG
ATAATATGCGTTTGGGACTGGAGCTGGAGAAATCAGCGTTCGGGAAATATAATGTGGATAATGCGATAAACGCCAACTTC
CGTTATGTTTTCTGA

Protein sequence :
MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVAALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTP
GTTNISIYDKQGNLVGKLDKAPMADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGGFLVGGTVSALNSYNNG
QMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYT
SGEGVLQWKYDAANGTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRVSALNGQTW
MGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDGTVILNQQADADGKVQAFSSVGIASGRPTVV
LSDSQQVNPDNISWGYRGGRLELNGNNLTFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAP
GDLYYDSSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQLNGNMDVNIPQLSGKD
VLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSDWETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTV
ILGSSRVFTDRSDGTGNAVFSVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRVGS
FVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTEGINLEDNASFSVKNMGYLSSDIHA
GTTAATINLGDSDADAGKTDSPLFSSLMKGYNAVLRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFA
TLQVKELSADNTTFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAGTQQIGFSNVT
PVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNNLNKRMGDLRDTQGDAGVWARIMNGTGSADGDY
SDNYTHVQIGVDRKHELDGVDLFTGALLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANF
ASLGTKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKDYNPLIGRTGVDVGRAFSG
DDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDSRMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANF
RYVF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pic NP_838464.1 serine protease precurser Virulence SHI-1 Protein 0.0 58
pic NP_708747.3 serine protease Not tested SHI-1 Protein 0.0 56
she AAB58244.1 mucinase Virulence SHI-1 Protein 0.0 56
pic AAK00464.1 Pic Virulence SHI-1 Protein 0.0 56
unnamed CAC39286.1 hypothetical protein Not tested LPA Protein 0.0 54
unnamed CAD66214.1 putative hemoglobin protease Not tested PAI III 536 Protein 0.0 47
vat YP_851472.1 vacuolating autotransporter Not tested PAI III APEC-O1 Protein 0.0 47
vat AAO21903.1 vacuolating autotransporter toxin Virulence Not named Protein 0.0 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
sepA NP_858203.1 extracellular serine protease SepA VFG0635 Protein 0.0 56
sepA NP_858203.1 extracellular serine protease SepA VFG0861 Protein 0.0 56
sepA NP_858203.1 extracellular serine protease SepA VFG0903 Protein 0.0 56
sepA NP_858203.1 extracellular serine protease SepA VFG0904 Protein 0.0 47
sepA NP_858203.1 extracellular serine protease SepA VFG1689 Protein 0.0 47