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Name : S3289 (S3289) Accession : NP_838565.2 Strain : Shigella flexneri 2457T Genome accession: NC_004741 Putative virulence/resistance : Unknown Product : insertion sequence 2 OrfB protein Function : - COG functional category : L : Replication, recombination and repair COG ID : COG2801 EC number : - Position : 3167507 - 3168358 bp Length : 852 bp Strand : + Note : IS2 OrfB; forms an overlapping reading frame with orfA; may form a fusion protein OrfAB due to ribosomal frameshifting; in some cases OrfB is itself expressed; both OrfA and OrfAB bind terminal inverted repeats DNA sequence : GTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTGTCGCGTGCGCA GTTGCACGTCATTCTCAGACGAACCGATGACTGGATGGATGGCCGCCGCAGTCGTCACACTGATGATACGGATGTGCTTC TCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGCTTCGCAGACAGGCAGAA CTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGCTCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAACC TGCTGTACCGCCATCGAAACGGGCACATACAGGCAGAGTGGCCGTGAAAGAAAGCAATCAGCGATGGTGCTCTGACGGGT TCGAGTTCTGCTGTGATAACGGAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGG GCGGTGACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGA TCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGA TGTTGGGACTTGAACCGAAGAACACGGCGGTGCGTAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATA AAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTA TAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGGGTAA Protein sequence : MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE LDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHW AVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPRG |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| S4050 | NP_839218.2 | insertion element IS2 transposase InsD | Not tested | SHI-2 | Protein | 9e-131 | 99 |
| SF3708 | NP_709447.1 | IS2 transposase TnpB | Not tested | SHI-2 | Protein | 9e-131 | 99 |
| SF3721 | NP_709460.1 | IS2 transposase TnpB | Not tested | SHI-2 | Protein | 9e-131 | 99 |
| S3189 | NP_838472.2 | insertion element IS2 transposase InsD | Not tested | SHI-1 | Protein | 1e-129 | 99 |
| S4061 | NP_839230.2 | insertion element IS2 transposase InsD | Not tested | SHI-2 | Protein | 2e-130 | 99 |
| tnpF | AAD44739.1 | TnpF | Not tested | SHI-2 | Protein | 5e-113 | 99 |
| SF2985 | NP_708759.1 | insertion element IS2 transposase InsD | Not tested | SHI-1 | Protein | 6e-111 | 99 |
| insD | YP_002414033.1 | insertion element IS2 transposase InsD | Not tested | Not named | Protein | 1e-126 | 97 |
| unnamed | CAC39284.1 | hypothetical protein | Not tested | LPA | Protein | 3e-111 | 97 |
| unnamed | AAL67379.1 | transposase-like protein | Not tested | PAI II CFT073 | Protein | 5e-109 | 96 |
| ECO111_3773 | YP_003236109.1 | putative IS2 transposase InsAB | Not tested | LEE | Protein | 3e-112 | 93 |
| aec49 | AAW51732.1 | Aec49 | Not tested | AGI-3 | Protein | 5e-114 | 85 |
| unnamed | CAC39283.1 | hypothetical protein | Not tested | LPA | Protein | 1e-81 | 62 |
| c5197 | NP_757045.1 | transposase insD | Not tested | PAI II CFT073 | Protein | 1e-61 | 60 |
| insD | YP_854263.1 | IS2 transposase | Not tested | PAI I APEC-O1 | Protein | 2e-65 | 60 |
| insD | AAZ04432.1 | IS2 transposase | Not tested | PAI I APEC-O1 | Protein | 1e-65 | 60 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| S3289 | NP_838565.2 | insertion sequence 2 OrfB protein | VFG0621 | Protein | 4e-131 | 99 |
| S3289 | NP_838565.2 | insertion sequence 2 OrfB protein | VFG0608 | Protein | 4e-131 | 99 |
| S3289 | NP_838565.2 | insertion sequence 2 OrfB protein | VFG0649 | Protein | 3e-111 | 99 |
| S3289 | NP_838565.2 | insertion sequence 2 OrfB protein | VFG1732 | Protein | 5e-127 | 97 |
