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Name : S1251 (S1251) Accession : NP_836865.2 Strain : Shigella flexneri 2457T Genome accession: NC_004741 Putative virulence/resistance : Unknown Product : insertion element IS2 transposase InsD Function : - COG functional category : L : Replication, recombination and repair COG ID : COG2801 EC number : - Position : 1210825 - 1211730 bp Length : 906 bp Strand : - Note : IS2 OrfB; forms an overlapping reading frame with orfA; may form a fusion protein OrfAB due to ribosomal frameshifting; in some cases OrfB is itself expressed; both OrfA and OrfAB bind terminal inverted repeats DNA sequence : GTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTGTCGCGTGCGCA GTTGCACGTCATTCTCAGACGAACCGATGACTGGATGGATGGCCGCCGCAGTCGTCACACTGATGATACGGATGTGCTTC TCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGCTTCGCAGACAGGCAGAA CTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGCTCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAACC TGCTGTACCGCCATCGAAACGGGCACATACAGGCAGAGTGGCCGTGAAAGAAAGCAATCAGCGATGGTGCTCTGACGGGT TCGAGTTCTGCTGTGATAACGGAGAGAGACTGCGTGTCACGTTCGTGCTGGACTGCTGTGATCGTGAGGCACTGCACTGG GCGGGGACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGA TCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGA TGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATA AAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTA TAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAA GTGATAACAGATGTCTGGAAATATAG Protein sequence : MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE LDGMPAINAKRVYRLMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFVLDCCDREALHW AGTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| SF3708 | NP_709447.1 | IS2 transposase TnpB | Not tested | SHI-2 | Protein | 6e-139 | 99 |
| SF3721 | NP_709460.1 | IS2 transposase TnpB | Not tested | SHI-2 | Protein | 6e-139 | 99 |
| S3189 | NP_838472.2 | insertion element IS2 transposase InsD | Not tested | SHI-1 | Protein | 1e-137 | 99 |
| tnpF | AAD44739.1 | TnpF | Not tested | SHI-2 | Protein | 3e-121 | 99 |
| S4061 | NP_839230.2 | insertion element IS2 transposase InsD | Not tested | SHI-2 | Protein | 1e-138 | 99 |
| S4050 | NP_839218.2 | insertion element IS2 transposase InsD | Not tested | SHI-2 | Protein | 6e-139 | 99 |
| SF2985 | NP_708759.1 | insertion element IS2 transposase InsD | Not tested | SHI-1 | Protein | 2e-119 | 99 |
| insD | YP_002414033.1 | insertion element IS2 transposase InsD | Not tested | Not named | Protein | 4e-135 | 97 |
| unnamed | AAL67379.1 | transposase-like protein | Not tested | PAI II CFT073 | Protein | 2e-117 | 96 |
| unnamed | CAC39284.1 | hypothetical protein | Not tested | LPA | Protein | 3e-118 | 96 |
| ECO111_3773 | YP_003236109.1 | putative IS2 transposase InsAB | Not tested | LEE | Protein | 2e-119 | 93 |
| aec49 | AAW51732.1 | Aec49 | Not tested | AGI-3 | Protein | 3e-121 | 84 |
| unnamed | CAC39283.1 | hypothetical protein | Not tested | LPA | Protein | 1e-83 | 62 |
| c5197 | NP_757045.1 | transposase insD | Not tested | PAI II CFT073 | Protein | 3e-61 | 59 |
| insD | YP_854263.1 | IS2 transposase | Not tested | PAI I APEC-O1 | Protein | 4e-65 | 59 |
| insD | AAZ04432.1 | IS2 transposase | Not tested | PAI I APEC-O1 | Protein | 3e-65 | 59 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| S1251 | NP_836865.2 | insertion element IS2 transposase InsD | VFG0608 | Protein | 3e-139 | 99 |
| S1251 | NP_836865.2 | insertion element IS2 transposase InsD | VFG0621 | Protein | 3e-139 | 99 |
| S1251 | NP_836865.2 | insertion element IS2 transposase InsD | VFG0649 | Protein | 1e-119 | 99 |
| S1251 | NP_836865.2 | insertion element IS2 transposase InsD | VFG1732 | Protein | 2e-135 | 97 |
