Gene Information

Name : lysS (BC0084)
Accession : NP_829979.1
Strain : Bacillus cereus ATCC 14579
Genome accession: NC_004722
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 80343 - 81842 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGATAACATGAACCACGAAGAATTAAACGACCAATTGATTGTTCGTCGTGAAAAGCTACATAATTTACGTGAACAAGG
AATTGACCCGTTCGGTAAAAGATTTGAACGCACAAATTCAACAACTGATTTAGTGAGTCTATATGGAGAGTTCTCTAAGG
AAGAATTAGAGGAGAAGGAAATCGCTGTTTCTATCGCTGGTCGTATTATGACAAAACGCGGAAAAGGGAAAGCTGGATTT
GCACACGTTCAAGATTTACATGGACAAGTTCAAATTTATGTTCGTAAAGATGCTGTTGGAGATGACGAGTACGAATTATT
TAAAACAGCAGATTTAGGTGATTTAGTAGGTATTGAAGGTAAAGTGTTCAAAACAAATGTTGGAGAACTTTCAGTAAAAG
CAACAGGCTTTACGCTATTAACGAAATCTCTTCGTCCATTACCAGATAAATACCATGGATTAAAAGATGTTGAACAACGA
TACCGTCAACGTTACTTAGACTTAATTACAAGTATGGAAAGTCGTGAAACGTTCGTTACTCGTAGTAAAATCATTCGTGA
GATGAGAAGATACCTAGATGACAACGGTTATCTTGAAGTGGAAACACCTATGATGCATGCAATTGCAGGTGGAGCTTCTG
CGCGTCCTTTCACTACACATCATAATGCGTTAGATATGGAATTATATATGCGTATCGCAATTGAGCTACATTTAAAGCGT
CTTATTGTGGGTGGTTTGGAAAAAGTATATGAGATCGGCCGTGTATTCCGTAATGAGGGTGTATCAACTCGTCATAACCC
TGAGTTTACGATGATTGAATTATATGAAGCATACGCTGATTATAAAGATATTATGAAGTTAACAGAAGATATGGTTGCTC
ATATCGCGAAAAAAGTACTGGGTACAACAACAATCCAGTATGGTGATTATGAAATTAATCTAGAACCAGAATGGACACGT
CTTCATATGGTAGATGCGATTAAGCAATACTCTGGAGCTGATTTCTGGAATCCGATGAGTGTAGAAGAAGCGCGTGAGCT
TGCAAAAGAACATAATGTAGAAATTAAAGATACAATGGAAGTTGGTCATATTATAAATGAATTCTTCGAACAAAAAGTAG
AAGATAAATTAATCCAACCAACATTTATTTACGGTCATCCGGTAGAAATCTCACCGCTTGCGAAAAAGAATGATGAAGAT
CCAAGATTTACAGATCGTTTCGAATTATTTATCGTTGCACGTGAACATGCAAATGCATTCACTGAGTTAAATGATCCAAT
CGATCAAAAAGAACGTTTTGAAGCGCAATTAAAAGAGCGCGAACAAGGTAATGATGAAGCTCATATGATGGATGACGATT
ATATTGAAGCTCTTGAGTACGGTATGCCTCCTACGGGTGGATTAGGAATCGGTATTGATCGTCTAGTTATGTTATTAACA
AATGCACCATCTATTCGTGATGTATTATTATTCCCTGCTATGCGCCATAAACAAGACTAA

Protein sequence :
MDNMNHEELNDQLIVRREKLHNLREQGIDPFGKRFERTNSTTDLVSLYGEFSKEELEEKEIAVSIAGRIMTKRGKGKAGF
AHVQDLHGQVQIYVRKDAVGDDEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFTTHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTEDMVAHIAKKVLGTTTIQYGDYEINLEPEWTR
LHMVDAIKQYSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-122 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS NP_829979.1 lysyl-tRNA synthetase VFG1668 Protein 8e-123 51