Gene Information

Name : EF1400 (EF1400)
Accession : NP_815121.1
Strain : Enterococcus faecalis V583
Genome accession: NC_004668
Putative virulence/resistance : Resistance
Product : cadmium-translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 1378053 - 1380155 bp
Length : 2103 bp
Strand : +
Note : similar to SP:P09123, and PID:452398; identified by sequence similarity

DNA sequence :
ATGCAAAAGAGTTATAAACTAGAAGGACTTGATTGTGCAAGTTGTGCAGACAAAATTGAAAAATCTGTCCAAAAAATTCA
CGGAGTAGAAAAAGCGCGTGTGGACTTCATGGCTGAAAAGATGACACTGGAAGTTGAATCAGGACATGATTTAGAAGTCG
AAAATGAAGCGCGTGCAGTGATTGGTAAGTTAGAATCAGATGTCAAAGTTATTTCTTTAAAAGATGTCAAAGAAGAAGAG
GGACGTAATCCAAATAAGAATCGCTTAATTCGTATCATTATTGCATTTGTTTTATTTTTAGCACTTATTATCATTAAACC
AAGTAATAATTGGGTTGCTTTGGCATCTTATTTAGTCGTTTATGTCTTAATTGGTGGCGACATTGTCAAACGAGCGGTAA
CGAATATTTTTCGTGGGGAAGTGTTTGATGAAAATTTCTTAATGTCTGTGGCAACGATCGGGGCATTCTTTATTGGGGAA
TATCCCGAAGCTGTGGCAGTAATGTTGTTCTACCAAGTCGGCGAATGGTTCCAAAGTGCTGCGGTTGATCAATCCAGAAA
ATCAATTGCCAAATTAATGGATATTCGTCCAGATTCAGCAAATCTTTTAGTAAATGGACAAATAAAAGCGGTCGCTCCAG
ATACAATTGAAATTGGGCAACAAATATTAGTGAAACCAGGTGAAAAAGTTCCATTAGATGGTCAAATTATTGATGGATCA
AGTATGGTAGATACCTCTGCGTTGACTGGTGAATCCGTTCCAAGAACGGTCAAAGTCGGCGATGAAATCTTAGGTGGTTT
TATCAATAAAAATGGGGCCTTGACCATTAATGTTACGAAAAAATTTGGGGATTCAACAGTTAGCAAAATTTTAGATTTAG
TTGAAAATGCTAGCAGTAAAAAAGCCCCTGCAGAAAACTTTATTTCTAAATTTGCTCGTTACTATACACCAGTTGTCGTT
GTTTTAGCTATCCTATTGGCAGTGATTCCACCATTTATTTTCCCTGATACTTCTATTAATGAATGGGTTTATCGAGCATT
AACGTTCTTGGTTATTTCTTGTCCATGTGCCTTAGTCATTTCTGTTCCTTTAAGTTTCTTCGGTGGAATTGGCGGAGCCA
GCAAACTAGGTGTTTTAATTAAAGGAAGTAACTATTTAGAAATTTTAGCGAATACGGAAACAATTGTTTTTGATAAAACT
GGTACGCTAACAAAAGGAAATTTTGTGGTTCAAAACATAACTAGTGTTGTCTTACCCGAAGAAGAGTTGTTGCGTTTAAC
AGCAACAGCAGAACAATTATCCACTCATCCAATTGCTATTTCTATTAAAGAAAGCTATGGCAAAGAAACTGTTCCAGCAA
CAGCAATTGAAGAGGTTGCCGGACACGGTATCAAAGCCACAATTGAAGGTAAAACAGTTTTAGTCGGTAATGCGAAGTTA
ATGAAACAATTTGGTATCGAAGCACCAGAAGTGAAGGAAGCGGGAACTTTAATTTTTGTCGCTATTGATAATCAATTTGC
AGGCTATTTAGTGATTGCGGATCAATTGAAACCAGACGCCATTTCAGCAATAAAAGAATTAAAGGCTGAAGGGGTCAAAC
AAACTGTTATGCTGACTGGAGACAATCAACAAGTTGCAGAAGCTATTGCAAAAGAAGTTGGTGTTGACAAAGTTTATGCA
GAACTTTTACCAGATGGTAAAGTGGATCGTTTAGAAGAGTTGTTAAAAGCATCTTCACCTAAAAACAAAGTTGCCTTTGT
TGGTGATGGTATGAATGATGCACCTGTTTTAGCCCGGGCTGATGTTGGGATTGCCATGGGTGGTTTAGGAAGTGACGCGG
CAATTGAAGCTGCTGATGTGGTGATTATGAATGATGAACCAAGTCGTATCGCCTCTGCGATTAAATTATCAAGAAAAACA
TTGAGAATTGTTAAACAAAACATTATCTTTGCGATTGCTGTTAAAATCATTGTCTTGGCACTAGGTGCATTAGGCTTGGC
TTCAATGCAAGCAGCAGTGTTTGCTGATGTTGGTGTAACTATTATCGCTGTTTTAAATGCTATGCGTTGTTTACGTGTAG
AAAAGATGAAAGATAATAACTAA

Protein sequence :
MQKSYKLEGLDCASCADKIEKSVQKIHGVEKARVDFMAEKMTLEVESGHDLEVENEARAVIGKLESDVKVISLKDVKEEE
GRNPNKNRLIRIIIAFVLFLALIIIKPSNNWVALASYLVVYVLIGGDIVKRAVTNIFRGEVFDENFLMSVATIGAFFIGE
YPEAVAVMLFYQVGEWFQSAAVDQSRKSIAKLMDIRPDSANLLVNGQIKAVAPDTIEIGQQILVKPGEKVPLDGQIIDGS
SMVDTSALTGESVPRTVKVGDEILGGFINKNGALTINVTKKFGDSTVSKILDLVENASSKKAPAENFISKFARYYTPVVV
VLAILLAVIPPFIFPDTSINEWVYRALTFLVISCPCALVISVPLSFFGGIGGASKLGVLIKGSNYLEILANTETIVFDKT
GTLTKGNFVVQNITSVVLPEEELLRLTATAEQLSTHPIAISIKESYGKETVPATAIEEVAGHGIKATIEGKTVLVGNAKL
MKQFGIEAPEVKEAGTLIFVAIDNQFAGYLVIADQLKPDAISAIKELKAEGVKQTVMLTGDNQQVAEAIAKEVGVDKVYA
ELLPDGKVDRLEELLKASSPKNKVAFVGDGMNDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDEPSRIASAIKLSRKT
LRIVKQNIIFAIAVKIIVLALGALGLASMQAAVFADVGVTIIAVLNAMRCLRVEKMKDNN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 2e-88 43
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 1e-88 43
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 4e-85 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
EF1400 NP_815121.1 cadmium-translocating P-type ATPase BAC0455 Protein 1e-142 58
EF1400 NP_815121.1 cadmium-translocating P-type ATPase BAC0055 Protein 4e-102 44