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Name : PSPTO_2602 (PSPTO_2602) Accession : NP_792411.1 Strain : Pseudomonas syringae DC3000 Genome accession: NC_004578 Putative virulence/resistance : Virulence Product : yersiniabactin non-ribosomal peptide synthetase Function : - COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism COG ID : COG1020 EC number : - Position : 2884461 - 2890634 bp Length : 6174 bp Strand : - Note : Catalyzes the condensation of salicylate with two molecules of cysteine which are both cyclized to thiazoline rings. This enzyme contains a single adenylation domain which most likely charges both of final two thiolation domains with cysteine. Following t DNA sequence : ATGTACACATCGACCAGAAAAGCCTCGACTGCTCGCGGTACGCCACCTCAAGCTTTCGACCCCGCCCTGCTGGGCGAGGA CATCGCCAGGCAACTGCGTCTGTCCCCGGAGCAGCTGGCGGATGACGCCAACCTGTTGAAGCTGGGCATGGACTCGATGC ACCTGATGGCCTGGCTCAACCGGTTTCGCCGTCTGGGTTTCAAAGTGACCCTCAGCGACCTCTATGACCAGCCGACACTG CAGGGCTGGCAGCAACTGCTGAGCAGCGCGCCCGTTCAAGTCAACAGCGCCGAGGTCGCCAGCAACGACGAGCCGCTTGC GCTCATGAGCGACGGCCAGCCGTTCGCCCTGACCAGTGTGCAGCATGCTTATCTAGTGGGGCGTTCCAGTGAACAGCCGC TGGGTGGCGTGGGTTGTCATCTTTATCAGGAATTCGACGGTCGCGGCCTGACACCACAGGTGCTGGAAGCCGCCATCTAT CAGTTGATCGAACGGCATCCGATGCTCAAGACGCGCTTTCTGGCCGACGGCCGTCAACAGTGGCAAGCGCATTCGACCTG GCCGGGCCTCAAGGTTCATGACCTGCGCGATAGCGATGACACGCTTCGCCAGCAGCGCCTGGGGGACCTGCGCGAACAGC TGGGCCATCGTCGCCTTGACGTCGAAAACGGCGAAACCTTCGACTTCCAGCTGTGCCTGTTGCCCGACGGTGGAAACCGG CTGTTGGTCAACATTGATCTGCTGGTTGCCGACGCAGCCAGCTTCAATCAGTTGTTCGAAGAACTGATCGCGTTGATCGA AGACCGGCCGTTGCCGCCGTCGCCTGTCGATTACGATTTCTGCAGTTACCTGACGCAGGTAAGGCGCAATGATCAGGCCC GCGTCGAGAAGGCCAAAGCCTGGTGGCTGGCACGTCTGGACGACCTGCCACTGGCACCGATTCTGCCGCTGGCGCAAGAC CCTGAGCGCATCGCGCAGGTTCGCATCAGCCGCCGTCGCGGACAACTGGACGCAGCGCAATGGCAGGCATTCAAGGACCA CGCCGGAACCGCTGGCGTTACGCCGACCATGGCGCTGGCCACGCTGTTCAGTGCAGTGCTGGGTCGCTGGAGCGGGCAAC AGTCGCTGCTGCTTAACCTCACCCTGTTTGACCGCCAGCCGCTGAACCCGGCAGTCGCCAGCATGATCGCCGATTTCACC AATATTCTCGCGTTGCCAGTGAGCTGCTGCGGGCAAGCCTTCGCAGAGTTGGCGCAAGCCAATCAGCAGTCGTTTGCCGA AGTTCACGAACACAGTGCGTGGTCGGGGGTCGAGGTGCTGCGCGAATTGAAGAAACGCCAGCGCCACCCGCACGGTGCGC CGCTGGTGTTCACCAGCAATCTGGGCCGCCCGCTGTTTGGCGAAGCCGCAGAGAACACGCTGGGCGCACCCGGCTGGGGC ATTTCCCAGACGCCGCAGGTGTGGATCGACCACCTGGCGTTCGAGCATCAGGGGCAGGTCTATCTGCAGTGGGACAGCAA TAACCAGTTGTTCCCCGAGGGGCTGACCGACACCTTGTTCGACGTCTATTTCGAACACGTGCTGGCGCTGGTCGCAGACC CCGCGCACTGGTCACACCCCTTGCCCGACCTGATGCCTTCCGCCCAGCGAGCCGTCCGCGAACAGGTCAATGCCACCGCC CGGGCCATTCCTGACGGGCTGCTGCACGACGCCGTTTTCCTCCAGGCCGAACGCACCCCGCAAGCCACCGCACTGATTCA GGACCAGCGCCGTCTGAGCTTCGCCCAACTGGCCGATCAGGCCAGTCGCCTGGCCGGCTGCCTGCAAGCCCTGGGTGTCA CAGCGGGCGACACCGTGGCGGTCAGCATGACCAAGGACATCGGCCAGATTGTCAGTGTGCTGGGCATTCTCAAGGCTGGT GCGATCTATGTGCCCGTACCGCCGGACCAGCCATTGGCGCGGCGTATCGGCATTTATCAGGGTGCGCAGGTGAAATGCGT GCTGACCAGCGCCGATGAGCCTGAAGAGCACGACATCGGCACGGTCCTGACCTGGCAGCAGGCAATCCTCAGCGAACCGC TTCAGCACCAGGTCCCGGTCAGCGCACAGCAACCGGCCTATATCATCTACACCTCAGGCTCGACCGGTGAACCCAAGGGC GTGGTGATCAGCCATCAAAGCGCCTTGAACACCTGCGTGGACATCAGCCAGCGCCACGCCGTGAGCCCCGAAGATCGTGT GCTGGCGCTGTCGGCGCTGCATTTTGACCTGTCGGTGTATGACATCTTCGGAGTGCTCGGCGCAGGCGCTTCGCTGGTGC TGGTCAACGACCGGCAACGTCGCGACCCGGCGCTGTGGTGCAGGCTGATCGATGAACACGGCATTACCCTATGGAACAGC GTACCTGCCCTGCTGGACATGTTGCTGACCTACAGCGAAGGCTTTGATCTGCACAGCCCGGCGAGCCTGAGGCTGGTGAT GCTGTCCGGCGACTGGATCGGCCTGGACCTGCCGGGGCGCTATCACCAGTACCGCAGCGATGGTCGGTTTGTCGCCATGG GCGGTGCGACGGAAGCGGCCATCTGGTCCAACACCTGCACCGTGAGCCGGGTTGAGCCGCACTGGCGCTCGATCCCCTAC GGCACGCCGCTGACCAACCAGCGTTATCGCGTGGTTGACGAAACAGGACGCGATTGCCCGGACTGGGTCGCCGGCGAGCT GTGGATCGGCGGCGCAGGTGTTGCACACGGCTATTTCAACGATGCCGAACGCAGCGCCCGGCAGTTCGTCGAAGTGGCCG GTGAACGCTGGTATCGCACCGGCGACATGGGCTGTTACTGGCCGGACGGAACGCTGGAATTCCTCGGCCGTCGTGACAAG CAGGTCAAGGTCGGTGGCTACCGCATCGAACTGGGTGAAATCGACGCAGCCTTGAACCGCCTGGAAGGTGTCAAGGCAGC CATCAGCATGGCACCGGGCGAACGGGAAAAAAGCCTGGTGGCTTTTGTCGTGCCGCACGGTTCCAGCCTGTGCAGCCGCG TCGAAGCCGACCCGTGCACACCCGCCAGTTACGCCGACTGGCTGACGCCTGTCACTGTCGCGACTGACACAACAGACGGT CTGCTGGCCGATTTCCTGCTTGAGCATCTGGCGTTCAACGGCATACCGTTCAGCCCCTCTCTGACGACCGACCAGGTGTT ACAGACCTACGGCAGCGAGGCGCGCTGGCACCCGCTTCTGACGCGCTGGCTGGAGTGGCTCGCAACACAGGATCGCCTGT GCTGCGACGTCGAGGGCGCGTGGCATCTCAATCATTCGAGCACAAAGGCCACGTCGCACGACGTTGCTTGCGATGCACTG ACACACGCCCTGCAGGCGCACCACGACGCCCTGCGCGACATACTGTCAGGCAAGCGCAGCCCTCAGACCCTGCTTGAGCA TCCGTACTGGGCTCCAGAACAGCTGCTGATGCACGCACCCGGCAGCCGCGAAACGCTCAGTGCACTTGCGACATCGCTGG CGGACCTGAGCAAGCGCTTGCAACGCCCGGTGCGCCTGATCGAAGCCGGTGCCCGCAGCGCTGTCACCGGGGCATTTCTG CTGGGGCGACTTGACGCAGAGCAGTTGCACTACACCGCCCTCGATACGTCACAGGCAATGGTCCTCAAGGCCCGCGAACG CCTCGCAGGTTTTATTCATGGCAGTGCACGCCGCGACACCGACAGCGAGCGCCAGGCGCTTGTTCACAGTGCCGACGTGC TGCTGATCAACAATCAATTACATCGTCTGGATATGCCGCAAGCAGCCTTACAGCAGTGGACGCCACTCTGCGCGCCAGGC GCTGCGGTCTATGTGCTGGAGCTGGCGCAAGCCTCGGCGCTGGCACTGGTCAGCGCCGAGTTGCTCAACGAAGGCCGCAG TGCAGCCGAACAATTGCGTAACAGCGAACACTGGACGGCGCTGCTGATGCAGGCCGGGCTTGGCGCCCCTGAAACGGATC GATCGGGCGCCCTGCAACGCCTGATCCTGCGCGCGCCCGATGTGCTGCAGCGCCCCGACTCTGCCCGGCTTGCCAAGGCG CTGGCCAACGAACTGCCCGGCTACATGATTCCGCAGCGCCTGTATTTTCTCGACGCACTGCCGTTGACCGCCAACGGCAA GATCGATCATCAGGCACTGATGCAGCGGTGCAAACCTGCCGCCACACAAGCCGACCAATGCCAGCCCCCTGAAAACGTCC GCGAACAGGTTCTCGCACAGCTCTGGCAGGAGTTGCTGCAAAGCGGCGCGGTGCACCGCCACAGCCAGTTCTTCCAGCTT GGCGGCGACAGCCTGCTGGCAACCCGCCTGATCGGCGAACTGGCCGGACGTGGCTATCACGCCCGGCTCGACGACCTTTT CAACTTCCCGAGCCTGCACGCCTTCGCGGCGACGCTGGTGGAGCACAATGCTCCGGCAACCGTGCGGCTTGAACACGACC CGGCGGGTCGTTATCAGCCCTTTGCCCTGAGTGAAGTGCAGCAGGCTTATCTGGTCGGGCGTCAGCCAGGATTCGTACTG AGCGGCGTGGGCGCGCATTTCTTTGTCGAGTTCAGCGTCGAACGACTTAATGTCCAGTTGTTTCAAAACGCCTGGCAGCG CCTCATCGAACGTCACGACATTCTGCGTGCTGTGGTCCGTGACGGCATGCTGCAGGTCCTGAAACAGGTCCAGGCCTTCA TTCCCCGGCGGCATCGGGTGGCCACGCTGGAGGGCAGCGAAGCCCATGCACTGCGCGAACGGCTTTCCCATCAGGTGCTC GATGCCGGGCAATGGCCACTGTTCGATGCGCAGATCGTCGAAGATGGCAGCCCGCACAGCCGCATTTTCGTCAGCCTCGA CAACCTGCTGCTCGACGGCATGAGCATGCAAATACTGTTGGCCGAACTTGAGACGCTGTACCTGCACCCCGAACAGACGC TGGAACCGATCGCTATCGGCTTTCGCGACTACCAGTGCCTGCGCGCCAGCCAGCCGCCCTCGCCCGGCGCTCGGGCGTAC TGGCAGCGGCGTATCGACAACCTGCCGCCAGCACCGCGCCTGCCACTGCGCTGCGCCCCAGCCGACGTCGGCATACCACG CTTTGTGCGCCTGGCCGAGCGTCTGCCAGCAGACCGTTGGGAGCGTTTGAAAGCCCAGGCCAATCAGCGCCAGTTGACGC CCTCAGGGCTACTGCTCAGCGCTTTTTCCGCCGTGCTGTCGGCCTGGAGCAGCGCGCCGGAGCTGACCCTCAACCTGACC TTGTTTGACCGTCAGCCGCTGCACACGCATATCGACCGGGTGATGGGCGATTTCACCTCGCTGCTGCTGCTGGCCTGGCA CCCCACCGCTGACTGGCTTGGCAGTGCGCAAAATCTACAGCAGCGGCTATGGCGCGATCTGGCCCAGCGCGATCACTCGG CGATCCGGGTCATGCGTGAACTGGCCAGCCGCCATGGCCTGGCTGCCGCGCAGATGCCGGTGGTCTTCACCAGTGCGCTG GGGTTCGACAAGGGCCGCTTCATGGCCCAATCGTCATGGCTCAAACCGGTCTGGGGCATCTCGCAGACGCCGCAGGTCTG GCTGGATCATCAGGTGTATGAGTCCGAAGGTGACCTGTGCCTTAACTGGGACGCGGTCGAGGCGCTGTTCGATCCCGACG TGTTGCGCGCCATGTTCGACCAGTACGTCAGCCTGCTGGAACGGCTGGCCGAAGACCCGCAGGCTTGGGGATTGTCCCTG GCGCAACTGGTTCCCCACGGACATCCTGGCGCTGACGTGGCTCCGTTGCCTCGCCCGCAACCGCTGCCATTACCACTGCC GCACGAGCCCGAACAACAGGCCGATGAACAGCTCGTCGACCAGATCCGGCATGCCTTTCATGAGGTGGTCGGCCTGAAAC TGCAGGACTGTCGGCAGAACTTCTTCGACGCAGGCGCCAGTTCGTTGAAACTGGTGCAGTTGCACGTGAAGCTGACCCAG CAGGGCCATCGGCAGTTGCAGGCCACTGACCTGTTCGGCTACCCGAATGCCCGCGCACTGGCGCGGCACTTGAGCCAGAC GCAACCGGCAAACGACACGCGGGACCAACCCCGACAGGCGCAACTGACGCAAAGAAACGCCAGAAGATTACGGCGCAACG GAGGTGGCTCATGA Protein sequence : MYTSTRKASTARGTPPQAFDPALLGEDIARQLRLSPEQLADDANLLKLGMDSMHLMAWLNRFRRLGFKVTLSDLYDQPTL QGWQQLLSSAPVQVNSAEVASNDEPLALMSDGQPFALTSVQHAYLVGRSSEQPLGGVGCHLYQEFDGRGLTPQVLEAAIY QLIERHPMLKTRFLADGRQQWQAHSTWPGLKVHDLRDSDDTLRQQRLGDLREQLGHRRLDVENGETFDFQLCLLPDGGNR LLVNIDLLVADAASFNQLFEELIALIEDRPLPPSPVDYDFCSYLTQVRRNDQARVEKAKAWWLARLDDLPLAPILPLAQD PERIAQVRISRRRGQLDAAQWQAFKDHAGTAGVTPTMALATLFSAVLGRWSGQQSLLLNLTLFDRQPLNPAVASMIADFT NILALPVSCCGQAFAELAQANQQSFAEVHEHSAWSGVEVLRELKKRQRHPHGAPLVFTSNLGRPLFGEAAENTLGAPGWG ISQTPQVWIDHLAFEHQGQVYLQWDSNNQLFPEGLTDTLFDVYFEHVLALVADPAHWSHPLPDLMPSAQRAVREQVNATA RAIPDGLLHDAVFLQAERTPQATALIQDQRRLSFAQLADQASRLAGCLQALGVTAGDTVAVSMTKDIGQIVSVLGILKAG AIYVPVPPDQPLARRIGIYQGAQVKCVLTSADEPEEHDIGTVLTWQQAILSEPLQHQVPVSAQQPAYIIYTSGSTGEPKG VVISHQSALNTCVDISQRHAVSPEDRVLALSALHFDLSVYDIFGVLGAGASLVLVNDRQRRDPALWCRLIDEHGITLWNS VPALLDMLLTYSEGFDLHSPASLRLVMLSGDWIGLDLPGRYHQYRSDGRFVAMGGATEAAIWSNTCTVSRVEPHWRSIPY GTPLTNQRYRVVDETGRDCPDWVAGELWIGGAGVAHGYFNDAERSARQFVEVAGERWYRTGDMGCYWPDGTLEFLGRRDK QVKVGGYRIELGEIDAALNRLEGVKAAISMAPGEREKSLVAFVVPHGSSLCSRVEADPCTPASYADWLTPVTVATDTTDG LLADFLLEHLAFNGIPFSPSLTTDQVLQTYGSEARWHPLLTRWLEWLATQDRLCCDVEGAWHLNHSSTKATSHDVACDAL THALQAHHDALRDILSGKRSPQTLLEHPYWAPEQLLMHAPGSRETLSALATSLADLSKRLQRPVRLIEAGARSAVTGAFL LGRLDAEQLHYTALDTSQAMVLKARERLAGFIHGSARRDTDSERQALVHSADVLLINNQLHRLDMPQAALQQWTPLCAPG AAVYVLELAQASALALVSAELLNEGRSAAEQLRNSEHWTALLMQAGLGAPETDRSGALQRLILRAPDVLQRPDSARLAKA LANELPGYMIPQRLYFLDALPLTANGKIDHQALMQRCKPAATQADQCQPPENVREQVLAQLWQELLQSGAVHRHSQFFQL GGDSLLATRLIGELAGRGYHARLDDLFNFPSLHAFAATLVEHNAPATVRLEHDPAGRYQPFALSEVQQAYLVGRQPGFVL SGVGAHFFVEFSVERLNVQLFQNAWQRLIERHDILRAVVRDGMLQVLKQVQAFIPRRHRVATLEGSEAHALRERLSHQVL DAGQWPLFDAQIVEDGSPHSRIFVSLDNLLLDGMSMQILLAELETLYLHPEQTLEPIAIGFRDYQCLRASQPPSPGARAY WQRRIDNLPPAPRLPLRCAPADVGIPRFVRLAERLPADRWERLKAQANQRQLTPSGLLLSAFSAVLSAWSSAPELTLNLT LFDRQPLHTHIDRVMGDFTSLLLLAWHPTADWLGSAQNLQQRLWRDLAQRDHSAIRVMRELASRHGLAAAQMPVVFTSAL GFDKGRFMAQSSWLKPVWGISQTPQVWLDHQVYESEGDLCLNWDAVEALFDPDVLRAMFDQYVSLLERLAEDPQAWGLSL AQLVPHGHPGADVAPLPRPQPLPLPLPHEPEQQADEQLVDQIRHAFHEVVGLKLQDCRQNFFDAGASSLKLVQLHVKLTQ QGHRQLQATDLFGYPNARALARHLSQTQPANDTRDQPRQAQLTQRNARRLRRNGGGS |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| irp2 | YP_002346902.1 | yersiniabactin biosynthetic protein | Virulence | HPI | Protein | 0.0 | 53 |
| irp2 | NP_669706.1 | HMWP2 nonribosomal peptide synthetase | Virulence | HPI | Protein | 0.0 | 53 |
| irp2 | YP_070124.1 | yersiniabactin biosynthetic protein | Virulence | HPI | Protein | 0.0 | 53 |
| irp2 | NP_993007.1 | yersiniabactin biosynthetic protein | Virulence | HPI | Protein | 0.0 | 53 |
| irp2 | CAA21390.1 | - | Virulence | HPI | Protein | 0.0 | 53 |
| irp2 | YP_853075.1 | yersiniabactin biosynthetic protein | Virulence | PAI IV APEC-O1 | Protein | 0.0 | 53 |
| irp2 | YP_001006815.1 | yersiniabactin biosynthetic protein | Virulence | HPI | Protein | 0.0 | 52 |
| PMI2599 | YP_002152317.1 | non-ribosomal peptide synthase | Not tested | Not named | Protein | 0.0 | 43 |
