Gene Information

Name : irp3 (PSPTO_2599)
Accession : NP_792408.1
Strain : Pseudomonas syringae DC3000
Genome accession: NC_004578
Putative virulence/resistance : Virulence
Product : yersiniabactin synthetase, thiazolinyl reductase component
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG4693
EC number : -
Position : 2872651 - 2873733 bp
Length : 1083 bp
Strand : -
Note : This enzyme reduces the carbon-nitrogen bond of the second of three thiazoline rings of the siderophore yersiniabactin. Most probably this reduction takes place while an intermediate of the biosynthesis is still bound to irp2, the non-ribosomal peptide sy

DNA sequence :
ATGAAAAGGCCATTGAAGGTGCTGGTTGTCGGGGCCAAGTTCGGGGAGCTGTACCTCAACAGTTTTCTGCTCGAACAGCC
CGGTTTGCAGCTGGCCGGGCTGCTGGCCAACGGCAGCCCGCGCGCCAGAGCGCTGGCCAACGCGTTCGGCATCGCGCTGT
ATACCGATATCGAGCAACTGCCGGACGACCTGGACATTGCCTGCGTGGTGGTGCGCTCGGCAGTGGTCGGCGGGGCCGGT
GGGCAATTGACCGAGAGCCTGCTGCGCCGAGGGTTGCATGTCATTCAAGAGCACCCGGTGCACCCGGATGAATTGGTTCG
GCATCAGCAACTGGCCACCCGGATGGGCTGCCAGTACATCGTCAACAGCTTCTACCCGCACACCGAGGCGGGCCGCTGCT
GGGCCGGCACGGCGCAACGCATCTCGCGGCTGCTGGAGGGCCGCAAGCCCAACCTGGCACAGCTGACCACCAGTCGCCAG
TTGCTCTACTCGGGCCTGGACCTGCTGTTTCAGGCGCTCGGCTGCGACCTGGCCAGGCAAGTCAGCGTCAGCCTGCTCGA
CGGCGACGATGACTTTCATACCCTGTCGCTGAACCTGCCGGACAGCCGCGTACTGCTGCGCCTGCAACGCTGGATGGCGG
CCGACGATCCGGACCTGCACAGCCTGGTCATGCACCAGCTCAATCTGGCGTGGCCCAGTGGCTACCTTTCGCTGGACGCC
ACTTACGGCCCGGTCAACTGGACACCTGCGCTGCACGCCGAGGCCCATGACGACGATCGTCAGACCCTCTACACCAGCAA
CGCCGACTATCTGCAGCACGCAACCGCGATGCCGCTGCACCCGGCAGCCAGTCACTGGCGTAGCGCCCATGAAATCGAAG
GGCCTGCCGGTGTCGGCTGGCTGCTGCAACAACTGCTGGCTGTCCTGCAAGGCCGGCCCCGCGCCGACGGCCTGCAGGCT
GATTATCAGTTGGCCGTGGCCAGGCTCTGGCAAGACATCCTGCGCTGCGCCGGACCCGCCGAGCAGCGCATGGCCAGCGC
GCCGCTTTTCATTGATGCCGCGCAATTGCGGGCGGAGGGTTGA

Protein sequence :
MKRPLKVLVVGAKFGELYLNSFLLEQPGLQLAGLLANGSPRARALANAFGIALYTDIEQLPDDLDIACVVVRSAVVGGAG
GQLTESLLRRGLHVIQEHPVHPDELVRHQQLATRMGCQYIVNSFYPHTEAGRCWAGTAQRISRLLEGRKPNLAQLTTSRQ
LLYSGLDLLFQALGCDLARQVSVSLLDGDDDFHTLSLNLPDSRVLLRLQRWMAADDPDLHSLVMHQLNLAWPSGYLSLDA
TYGPVNWTPALHAEAHDDDRQTLYTSNADYLQHATAMPLHPAASHWRSAHEIEGPAGVGWLLQQLLAVLQGRPRADGLQA
DYQLAVARLWQDILRCAGPAEQRMASAPLFIDAAQLRAEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtU CAA21392.1 - Virulence HPI Protein 9e-61 50
irp3 CAA73128.1 Irp3 protein Virulence HPI Protein 4e-62 50
irp3 YP_002346900.1 yersiniabactin biosynthetic protein YbtU Virulence HPI Protein 9e-61 50
ybtU NP_669708.2 thiazolinyl-S-HMWP1 reductase Virulence HPI Protein 9e-61 50
ybtU NP_993005.2 yersiniabactin biosynthetic protein YbtU Virulence HPI Protein 9e-61 50
ybtU YP_070122.1 yersiniabactin biosynthetic protein YbtU Virulence HPI Protein 9e-61 50
irp3 YP_001006817.1 yersiniabactin biosynthetic protein YbtU Virulence HPI Protein 5e-62 50
ybtU YP_853077.1 yersiniabactin biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-60 50
nrpU YP_002152319.1 siderophore biosynthetic protein Virulence Not named Protein 8e-65 45