Gene Information

Name : hrcN (PSPTO_1400)
Accession : NP_791227.1
Strain : Pseudomonas syringae DC3000
Genome accession: NC_004578
Putative virulence/resistance : Virulence
Product : type III secretion cytoplasmic ATPase HrcN
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 1537029 - 1538378 bp
Length : 1350 bp
Strand : -
Note : see PMID:20190049 for expression data; similar to GP:11276511, GB:J04478, GB:M11718, GB:X04758, GB:M10956, GB:M11135, SP:P05997, PID:1340175, PID:179694, PID:179698, PID:180428, PID:180913, PID:2370202, and PID:553235; identified by sequence similarity; p

DNA sequence :
GTGAACGCCGCACTGAACCAGTGGAAAGACACCCACGCAGCGCGCTTGAGCCAGTACTCCGCCGTGCGCGTCAGTGGCCG
GGTGAGCGCGGTGCGCGGGATTTTGCTGGAGTGCAAGATACCGGCAGCCAAGGTCGGCGATCTGTGCGAAGTGAGCAAAG
CCGACGGCAGTTTTCTGCTGGCTGAAATCGTTGGCTTCACCCAGGAATGCACGCTGCTCAGCGCCCTCGGTGCGCCGGAC
GGCATTCAGGTCGGTGCGCCGATCCGCCCGCTCGGCATCGCCCATCGAATAGGCGTGGACGACACGTTGCTGGGCTGCGT
GCTGGACGGTTTCGGACGGCCGCTGCTCGGTGATTGCCTGGGGGCCTTTGCCGGGCCGGATGATCGCCGCGACACGCTGC
CGGTGATTGCCGACGCCTTGCCGCCAACCCAGCGGCCGCGCATCACCCGGTCTTTGCCTACCGGCATACGGGCCATCGAC
AGCGCCATTCTGCTCGGTGAAGGCCAGCGGGTCGGGCTGTTCGCCGGTGCCGGTTGCGGCAAGACCACGCTGATGGCCGA
ACTGGCCCGCAACATGGACTGCGACGTCATCGTCTTCGGGCTGATCGGTGAGCGGGGCCGCGAACTGCGCGAGTTTCTCG
ACCATGAACTGGACGAAACCCTGCGCGCTCGCTCGGTACTGGTTTGTGCCACGTCCGATCGATCCAGTATGGAACGCGCC
CGCGCCGCTTTCACGGCAACCGCCATCGCCGAAGCCTTTCGCGCGCGGGGCCAGAAGGTCTTGCTGCTGCTCGACTCCCT
GACCCGATTTGCCCGTGCGCAACGGGAAATCGGCATTGCATCGGGCGAACCGCTGGGCCGCGGCGGTCTGCCACCCTCGG
TGTACACCCTGCTGCCACGGCTGGTGGAACGTGCCGGGATGAGCGAAAACGGTTCGATCACCGCGCTCTACACCGTACTG
ATCGAGCAGGACTCGATGAACGACCCCGTGGCCGATGAAGTACGCTCGTTGCTCGACGGCCATATCGTGCTGTCGCGCAA
GCTGGCCGAGCGCGGCCACTACCCCGCTATCGACGTGTCCGCCAGCATCAGCCGGATCCTCAGCAACGTCACCGGTCGCG
ATCACCAGCGCGCCAACAATCGTCTGCGCCAGTTGCTGGCGGCCTACAAGCAAGTGGAAATGCTCTTGCGCCTGGGTGAA
TACCAGGCCGGCGCCGACCCGGTGACCGATTGCGCGGTGCAACTGAACGACGCGATCAATGCGTTTCTGCGCCAGGACCT
GCGTGAGCCTGTGCCCCTGCAGGAAACCCTCGACGGGTTGCTGCGCATCACCTCGCAACTGCCGGAGTAA

Protein sequence :
MNAALNQWKDTHAARLSQYSAVRVSGRVSAVRGILLECKIPAAKVGDLCEVSKADGSFLLAEIVGFTQECTLLSALGAPD
GIQVGAPIRPLGIAHRIGVDDTLLGCVLDGFGRPLLGDCLGAFAGPDDRRDTLPVIADALPPTQRPRITRSLPTGIRAID
SAILLGEGQRVGLFAGAGCGKTTLMAELARNMDCDVIVFGLIGERGRELREFLDHELDETLRARSVLVCATSDRSSMERA
RAAFTATAIAEAFRARGQKVLLLLDSLTRFARAQREIGIASGEPLGRGGLPPSVYTLLPRLVERAGMSENGSITALYTVL
IEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVSASISRILSNVTGRDHQRANNRLRQLLAAYKQVEMLLRLGE
YQAGADPVTDCAVQLNDAINAFLRQDLREPVPLQETLDGLLRITSQLPE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 0.0 100
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-180 100
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 0.0 94
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 0.0 94
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 8e-162 88
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 8e-162 88
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-144 85
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-126 66
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 8e-130 66
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-127 66
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-127 66
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-127 66
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-89 54
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-80 52
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-80 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-79 52
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 7e-80 52
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-80 52
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-80 52
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-80 52
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-77 52
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-77 52
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-75 51
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 7e-75 51
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-75 51
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-74 51
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-74 51
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 4e-76 50
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-68 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-68 45
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 3e-63 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-62 44
escN AAK26715.1 EscN Virulence LEE Protein 7e-68 44
escN AAL57542.1 EscN Virulence LEE Protein 7e-68 44
escN AAC38383.1 EscN Virulence LEE Protein 7e-68 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-67 44
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-65 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-67 44
escN AAC31513.1 L0034 Virulence LEE Protein 2e-67 44
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-67 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-67 44
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-67 44
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-67 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 8e-68 44
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-60 43
escN YP_003236088.1 translocator EscN Virulence LEE Protein 6e-67 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-58 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 6e-58 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-58 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-58 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG0192 Protein 2e-87 55
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG0390 Protein 1e-83 53
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG0048 Protein 5e-85 52
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG0515 Protein 1e-74 51
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG2144 Protein 5e-79 46
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG1254 Protein 5e-67 46
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG2331 Protein 3e-63 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG2499 Protein 4e-65 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG0730 Protein 4e-68 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG0812 Protein 1e-67 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG2459 Protein 2e-63 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG0555 Protein 2e-58 42
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG1008 Protein 3e-60 42
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN VFG1778 Protein 1e-58 41