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Name : lysS (CTC00211) Accession : NP_780922.1 Strain : Clostridium tetani E88 Genome accession: NC_004557 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 170435 - 171943 bp Length : 1509 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCAAATGAAGAAAAGAATTTAATGAATGAAGAACAAGAATTTAATCAGCTTATTCAAGAAAGAAGACAGAAGTTTTT TGATCTTCAGGCTGAAGGAAAAGATCCTTTTGAAGTAACAAAAGTAGAAAGAACTCATACTTCCGAAGAAATAAAAAGTA ATTTTGAAGAACTAGAAGATAAAGAGGTTACTGTAGCTGGAAGACTAATGTCTAAAAGAGTACATGGTAAAGCTGGTTTT TCCGATATTCATGATAGATATGGGAAAATTCAACTTTATATTAAAATAAATGATGTAGGAGAAGAAAGATTAAAAGAATA TAAATCTTTTGATATAGGAGATTTTGTTACTATTACAGGAACGGTATTTAAAACGAGAACGGGAGAAGTTTCTATACACA TAACAGACTTTAAGCTTATAGCAAAGTCTTTAAAGCCATTACCAGAAAAATGGCATGGACTAAAAGATCCGGATTTAAGA TATAGACAAAGATATGTAGATTTAATTGTAAACGAAGATGTAAGAGATACTTTCATGAAAAGAACTAAAATAATAAAATA TATGAGAGAATTTCTAGATGCTAAAGATTACATAGAAGTAGAAACACCTGTACTATCACCAATAGCAGGGGGAGCAGCAG CTAGACCTTTTACAACACATCACAATGCATTAGATATAGATATGTATTTAAGAATTGCTACTGAACTTTATTTGAAAAGA TTAATAGTTGGTGGATTTGAAAAAGTATATGAAATAGGTAAAAACTTTAGAAATGAAGGAATAGATATTAGACATAACCC TGAGTTTACAATGATTGAATTATATGAAGCATATGCAGATTATAATGATATGATGGAAATAACAGAAAATATGGTAGCTT ATATAGCGGAAAAGGTTTGTGGAAATACAAAAGTAAAATATGAAGAAACAGAAATAGAATTTAGACCTCCGTGGAAGAGA ATCACAATGGTAGACTCTGTTAAAGAATATTCTGGAGTAGATTTCAACGAAATAAAAACAGATGAAGAAGCAAGAGAGCT TGTTAAAGGAAAAGACTTTGAATTAAAGAAAAAATTAGAAGACTGTACAAAAGCAGATATTTTAAATCTTTTCTTTGAAG AATATGTTGAAAAGAATTTAATTCAACCTACTTTTGTATGTGATTATCCAGTTGAAATATCTCCTTTAAGTAAGAAAAAG AATGATAACCCTGAGTATACAGAAAGATTTGAAGGATTTGTGTACGGAAGAGAATTATGTAATGCATATACTGAGTTAAA TGATCCTATAGTACAAAAAGAGAGATTTATTCAACAATTAAAAGAAAGAGAATTAGGTGATGACGAAGCATTTATGATGG ACGAAGACTTTATTAATGCTTTAGAAATAGGTATGCCTCCGACAGGTGGCTTAGGGATAGGAGTAGACAGATTAGTTATG TTCTTAACAGATTCTCATTCAATAAGAGATGTAATTCTATTCCCAACTATGAAGCCAACACATAATTAA Protein sequence : MSNEEKNLMNEEQEFNQLIQERRQKFFDLQAEGKDPFEVTKVERTHTSEEIKSNFEELEDKEVTVAGRLMSKRVHGKAGF SDIHDRYGKIQLYIKINDVGEERLKEYKSFDIGDFVTITGTVFKTRTGEVSIHITDFKLIAKSLKPLPEKWHGLKDPDLR YRQRYVDLIVNEDVRDTFMKRTKIIKYMREFLDAKDYIEVETPVLSPIAGGAAARPFTTHHNALDIDMYLRIATELYLKR LIVGGFEKVYEIGKNFRNEGIDIRHNPEFTMIELYEAYADYNDMMEITENMVAYIAEKVCGNTKVKYEETEIEFRPPWKR ITMVDSVKEYSGVDFNEIKTDEEARELVKGKDFELKKKLEDCTKADILNLFFEEYVEKNLIQPTFVCDYPVEISPLSKKK NDNPEYTERFEGFVYGRELCNAYTELNDPIVQKERFIQQLKERELGDDEAFMMDEDFINALEIGMPPTGGLGIGVDRLVM FLTDSHSIRDVILFPTMKPTHN |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-107 | 48 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | NP_780922.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 8e-108 | 48 |
