Gene Information

Name : lysS (PD0404)
Accession : NP_778635.1
Strain : Xylella fastidiosa Temecula1
Genome accession: NC_004556
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 494346 - 495866 bp
Length : 1521 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGAGCATCTCCCTGCGTCGCAGGTGTCCTTTGACGAGAACGCGCTGATTGCCGAGCGCCGTGCAAAACTTTTAGC
ACTACGTGCGCAAGGCGTGGCTTATCCGAATGATGTCAAACGCGAGCATTATGCTGCTGATGTGCAGGCAGCATTTGCCA
ACGTCGAGACATGGACTGCTGAGACCCTGGAGGCGTCAAGCCATCGTGTACGTATGGCTGGCCGATTGATGGCTAAGCGT
CTCATGGGCAAGGCCAGCTTTGCTCAGATTCAGGACGAGTCTGGCCGCATCCAGCTGTTAATACAGAGTAACGTGCTTGG
TGAGGACAGCTATGCCGCATTCAAGGTGTTGGATGTTGGCGACATCATTGCTGTTGAAGGAGGATTGACCCGCACCAGAA
CGGGCGAGCTGTCGGTCAAGGTGAATGTTTTGCGACTTTTGACGAAGGCGTTACGTCCATTGCCTGACAAGTGGCACGGG
CTGACTGATGTGGAGCAGCGCTACCGCCAGCGCTATGTTGATCTTATTGTGACTCCAGAATCGCGTGAGATCTTCATCAA
ACGCTCCAAGATCATTCGTGCGCTGCGTACTTGGCTGGATGCGCGTCTTTTCTTGGAAGTAGAGACGCCGATGATGCATT
ACATCCCAGGAGGTGCTGCGGCTAAGCCGTTTGTGACCTACCACAATGCCTTGGATTTAGAGTTATATCTGCGCGTGGCC
CCAGAGTTGTATCTCAAGCGTTTGGTCGTGGGTGGGCTGGAACGGGTTTACGAGATTAACCGCAACTTCCGTAACGAGGG
GGTGTCGACCCGGCACAACCCCGAATTTACGATGCTTGAGTTATACGAGGCCTATTCCACCTACCATGAGGTCATGGATC
TGGCTGAGACGATGATCCGTGATACTGCACAGTCTGTGCTGGGCACGACCCAGGTGATTTGGGACGGTGCTCAGATTGAT
CTGGGTCCGATATTCCGGCGTTGGCGCATGGATGAGGCAGTTTGCCATCATAATCCTGAGATGAGCGTTGCCGAGTGTAC
CGATCGTGATGCATTGCTCCTTCACTGTGAGAGACTCAAGATCAAGGTTAAATCGTCCTACGGTTGGGGTCGATTGCTCT
TGAGTATTTTTGAGGCGACTGTTGAGCATACGTTGATCCAGCCGACCTTCATTACGGATCATCCGGTTGAAATCTCTCCT
TTAGCGCGCGAAAGCGACATCGAATCAGGGTATACCGATCGCTTCGAACTGTTCATCAATGGGAAGGAAATCGCCAACGG
TTTCTCGGAATTGAACGATCCGGAGGAGCAGGCGATGCGCTTCCAAAAGCAGGTTGAAGCCAAAGAAGGGGGGGATGATG
AGGCCATGTACTACGATGCCGACTATATTCGCGCTTTGGAGTATGGGATGGCGCCAACCGGAGGGTTAGGGATTGGTGTT
GATCGACTGGTGATGCTGCTGACCGGTAGCACCTCAATCCGCGATGTGCTGTTATTCCCTTATATGCGTCCAGAACGTTA
A

Protein sequence :
MTEHLPASQVSFDENALIAERRAKLLALRAQGVAYPNDVKREHYAADVQAAFANVETWTAETLEASSHRVRMAGRLMAKR
LMGKASFAQIQDESGRIQLLIQSNVLGEDSYAAFKVLDVGDIIAVEGGLTRTRTGELSVKVNVLRLLTKALRPLPDKWHG
LTDVEQRYRQRYVDLIVTPESREIFIKRSKIIRALRTWLDARLFLEVETPMMHYIPGGAAAKPFVTYHNALDLELYLRVA
PELYLKRLVVGGLERVYEINRNFRNEGVSTRHNPEFTMLELYEAYSTYHEVMDLAETMIRDTAQSVLGTTQVIWDGAQID
LGPIFRRWRMDEAVCHHNPEMSVAECTDRDALLLHCERLKIKVKSSYGWGRLLLSIFEATVEHTLIQPTFITDHPVEISP
LARESDIESGYTDRFELFINGKEIANGFSELNDPEEQAMRFQKQVEAKEGGDDEAMYYDADYIRALEYGMAPTGGLGIGV
DRLVMLLTGSTSIRDVLLFPYMRPER

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-127 54
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-79 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS NP_778635.1 lysyl-tRNA synthetase VFG1668 Protein 3e-127 54