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Name : lysS (ECA0774) Accession : YP_048886.1 Strain : Pectobacterium atrosepticum SCRI1043 Genome accession: NC_004547 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 844582 - 846099 bp Length : 1518 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGGCTGAATCACAATCACAGGGTGCCGATCAGGCGCAAGATCTGAATAATGAATTAAAAGCGCGTCGTGAAAAGCTGGT GACGCTGCGTGAAAACGGGATCGCATTCCCGAATGATTTCCGCCGTGACAGCATCTCCGATCACTTGCACGCTGAGTTCG ATGCCAAAGAGAACGAAGAGCTGGAAGAACTGGGTATCGAAGTGACCGTTGCGGGCCGTATGATGACCCGTCGCATCATG GGTAAAGCCTCTTTCGTAACCTTGCAGGACGTCGGTGGCCGCATTCAGCTGTATGTTTCCCGCGACGATCTGGCGGAAGG CATCTATAACGAGCAGTTCAAGAAATGGGATCTGGGCGATATCCTGGGTGCGCGCGGCAAGCTGTTCAAAACCAAAACGG GCGAGCTGTCCATTCACTGTACCGAGCTGCGTCTGCTGACCAAAGCGCTGCGCCCGCTGCCGGATAAATTCCACGGTCTG GCCGATCAGGAAACCCGCTATCGTCAGCGCTATCTGGATCTGATCGCTAACGACGACTCTCGTAATACCTTCCGCATCCG TTCTAACGTGATGGCGGCCATTCGCCGCTTCATGGTGGATAACGGCTTTATGGAAGTGGAAACGCCGATGATGCAGGTGA TCCCCGGCGGCGCGTCTGCTCGTCCGTTTATCACGCACCATAACGCGCTGGACATTGACATGTACCTGCGTATTGCACCG GAACTGTATCTGAAGCGTCTGGTTGTAGGTGGTTTTGAGCGTGTGTTCGAGATTAACCGTAACTTCCGTAACGAAGGTGT TTCCCCACGTCACAACCCTGAATTCACCATGATGGAACTTTATATGGCGTATGCCGATTATAAAGACCTGATTGTGTTGA CGGAAAACCTGTTCCGTACGCTGACGCAGGACGTGCTGGGCTCGACGACGGTGGTGTACGGCGATCAGACATTCGACTTC GGTAAGCCGTTTGATAAGCTGACGATGCGCGAAGCGATCTGTAAATACCGTCCTGAAACCAACGTTGCCGATCTGGACGA TCTGGAGAAAGCGACCGCGATCGCACTGTCTCTGGGCATCAAGATCGAGAAAAGCTGGGGCTTGGGACGCATCGTTACCG AGATCTTCGAAGAGACAGCAGAAAGCAGCCTGATCCAACCAACCTTCATCACCGAATATCCGGCTGAAGTGTCGCCGCTG GCGCGTCGTAACGATCAGAACTCGGAAATCACCGATCGCTTTGAGTTCTTCATCGGCGGTCGTGAAATTGGCAACGGTTT CTCCGAGCTGAATGATGCCGAAGATCAAGCAGAGCGTTTTGCTCAGCAGGTCAATGCTAAAGATGCGGGCGATGACGAAG CGATGTTCTACGACGAAGACTACGTGACGGCGTTGGAGCACGGTTTGCCGCCGACAGCGGGTCTGGGTATTGGTATCGAC CGTATGGTGATGTTGTTCACGAACAGCCACACCATTCGCGACGTCATCCTGTTCCCAGCGATGCGTCCGCAGAAATAA Protein sequence : MAESQSQGADQAQDLNNELKARREKLVTLRENGIAFPNDFRRDSISDHLHAEFDAKENEELEELGIEVTVAGRMMTRRIM GKASFVTLQDVGGRIQLYVSRDDLAEGIYNEQFKKWDLGDILGARGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL ADQETRYRQRYLDLIANDDSRNTFRIRSNVMAAIRRFMVDNGFMEVETPMMQVIPGGASARPFITHHNALDIDMYLRIAP ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMELYMAYADYKDLIVLTENLFRTLTQDVLGSTTVVYGDQTFDF GKPFDKLTMREAICKYRPETNVADLDDLEKATAIALSLGIKIEKSWGLGRIVTEIFEETAESSLIQPTFITEYPAEVSPL ARRNDQNSEITDRFEFFIGGREIGNGFSELNDAEDQAERFAQQVNAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGID RMVMLFTNSHTIRDVILFPAMRPQK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 3e-165 | 68 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 3e-88 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_048886.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 2e-165 | 68 |
