Gene Information

Name : topB (ECA0525)
Accession : YP_048644.1
Strain : Pectobacterium atrosepticum SCRI1043
Genome accession: NC_004547
Putative virulence/resistance : Unknown
Product : DNA topoisomerase III
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0550
EC number : -
Position : 599597 - 601615 bp
Length : 2019 bp
Strand : +
Note : decatenates replicating daughter chromosomes

DNA sequence :
ATGATGCGACTTTTTCTTTGTGAAAAGCCGAGCCAGGGGCGAGATATCGCCCGTTTTATCGGTGCAACTCAACGAGGGAA
AGGTTTTTTATCAGGCACCGGAACTGTCGTAACTTGGGCGTTTGGGCATCTTCTGGAAGTCGCCTCACCTGAAGAATACG
GTGCTCAGTTCGGCAAACCCTGGCGTATGGATGTTCTACCTATTCTGCCAACAGAGTGGAAAATGGTTGTTAAGCAAAGT
TCTGCTGATCAATTCGCTGTAATTAGTCGATTACTGAAACAGGCAGATGAAGTTGTTATTGCGACAGATGCCGATAGAGA
AGGAGAAGTACTGGCGAGAGAGCTATTGGATTATTGTCATTTTACTGGGAATGTAAAACGTCTGTGGCTTAGTGCTCTTG
ACGATGCCAGCATCCGAAAGGGATTAGATAACCTTCTCCCAGGCGAGAAAACAGTTCGTCTTTATTATGCTGGCATGGGG
CGTAGCAAGGCTGACTGGCTGATTGGTATGAATTTAACTCGAATATATACCCTTAAAGCTTCAGAACAAGGTATCGATGA
GCTGTTGTCTATAGGACGAGTTCAAACCCCAACACTGGCGCTTGTTGTCCAGAGAGATAGCGATATTGAGAATTTCTCTC
CTAAGCCCTACTGGCAGGTGTTTGCTGATATTGAAAAAGCCGGAATGACCTTTAGAGCTCAATGGGTACCAGCTGCTGGA
TATTGCGATGATGAAAAGCGCTGTATTCAGCATAATGCAGCCCTTGCAGTGGAGCAGCTCTGCAAGCAAACAGCAACAAC
ATCTGTCGTTGATGTGTTACTTAAGAGAGAAAAAACGCCAGCGCCGTTATGCTTTAGCTTGAGCGTGTTGCAGAAAGCCT
GTTCTGATAAATGGGGAATGATCGCCCAGAAGGTATTAGAAATCGCTCAGTCACTTTATGAGGTTCACAAAGCGACGACT
TATCCCAGAACTGATTGTGGATACTTGCCTGAGTCCATGAAAGGGGAGGTGAGCGATGTGCTTTCCGCTATTTTACAGTC
AGATCCTGCCGCCACATCACTTATTAAAGATGCTGATCGTTCTTTTGTTTCCCGTATCTGGAATGACAAAAAAATTTCTG
CTCACCATGCAATTATTCCTACGAAACAACCCTTCGATATAAGCAAATTATCAGATGATGAGTTGAATGTTTATCAACTC
ATTCGTCTGCATTATTTTGCTCAGTTTTTTCCTCTCCATGAGTCCGATGTTACAGATGTTACATTTAATTTAGGTGGGCA
ACTATTTAAAACTCGAGGTAGGGTGAGTGTCGTTATTGGATGGAAGAAACTCTTTTTCAATGATAAAGAGCCCGATATTC
AATCTGATGAAAATGGAAGTCAAGGGAGCCTTCCTGTTTTACATAACGGTGATATTTGTAATGTATTGGATTGTGAAGTT
AAAGCCCAACAGACTAAACCGCCATCTCGATTTACTGATGGCACATTGATTGATGCAATGAAGAATTCAGCTAGTTTTAT
TAGTGATCCGACATTAAAGAAAATTTTACGTGATAATGCGGGGCTGGGTACCGAGGCTACTCGTGCAAATATATTAGATG
TACTATTTAAGCGTAATTACCTAAAACGGCGTGAAAAGTTCATTTATTCAACGCAGCTGGCCAGAGAGCTAATCTCTCAA
TTACCAGAGGCTCTAACTAATCCAGGCATGACAGCGCTTTGGGAACAGGCATTGGATGATGTTGCTGAAGGACGCATGTC
GTTGGATGCATTTTTACAAAAGCAAAAAGAATGGACATTAAGATTAGTAGATAGGGGGCGTCAACAAGCGATTAAGTTGA
CGGCACCAGTCTCTCCATTGTGTCCCCAGTGTGGTGGGATAATGAGAAAAAGAAACAGTGATAATGGTGTGTTTTGGGGG
TGTGTGAAGTACCCGCTCTGTAAAGGGATCATTAGCGAAGGGACAAAAGGTAAGAGGGGTAAGAAAAATCTGAAAACTAA
GAATAAACCAGCTACCTGA

Protein sequence :
MMRLFLCEKPSQGRDIARFIGATQRGKGFLSGTGTVVTWAFGHLLEVASPEEYGAQFGKPWRMDVLPILPTEWKMVVKQS
SADQFAVISRLLKQADEVVIATDADREGEVLARELLDYCHFTGNVKRLWLSALDDASIRKGLDNLLPGEKTVRLYYAGMG
RSKADWLIGMNLTRIYTLKASEQGIDELLSIGRVQTPTLALVVQRDSDIENFSPKPYWQVFADIEKAGMTFRAQWVPAAG
YCDDEKRCIQHNAALAVEQLCKQTATTSVVDVLLKREKTPAPLCFSLSVLQKACSDKWGMIAQKVLEIAQSLYEVHKATT
YPRTDCGYLPESMKGEVSDVLSAILQSDPAATSLIKDADRSFVSRIWNDKKISAHHAIIPTKQPFDISKLSDDELNVYQL
IRLHYFAQFFPLHESDVTDVTFNLGGQLFKTRGRVSVVIGWKKLFFNDKEPDIQSDENGSQGSLPVLHNGDICNVLDCEV
KAQQTKPPSRFTDGTLIDAMKNSASFISDPTLKKILRDNAGLGTEATRANILDVLFKRNYLKRREKFIYSTQLARELISQ
LPEALTNPGMTALWEQALDDVAEGRMSLDAFLQKQKEWTLRLVDRGRQQAIKLTAPVSPLCPQCGGIMRKRNSDNGVFWG
CVKYPLCKGIISEGTKGKRGKKNLKTKNKPAT

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topB AAF14809.1 topoisomerase B Not tested SPI-7 Protein 0.0 62
topB NP_458624.1 topoisomerase B Not tested SPI-7 Protein 0.0 62
topB NP_807836.1 DNA topoisomerase III Not tested SPI-7 Protein 0.0 62
PMI2630 YP_002152332.1 DNA topoisomerase III Not tested Not named Protein 0.0 55
pph56 CAI36097.1 Topoisomerase IA Not tested PPHGI-1 Protein 8e-159 49
topB1 YP_005176228.1 DNA topoisomerase III Not tested ICEPmu1 Protein 3e-151 49
topB AAN62194.1 putative DNA topoisomerase III Not tested PAGI-2(C) Protein 1e-158 47
topB AAN62317.1 putative DNA topoisomerase III Not tested PAGI-3(SG) Protein 6e-154 47
topB AAW83109.1 TopB Not tested GGI Protein 6e-127 42