Gene Information

Name : entE (ECA0478)
Accession : YP_048596.1
Strain : Pectobacterium atrosepticum SCRI1043
Genome accession: NC_004547
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 544947 - 546572 bp
Length : 1626 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGAGCATTGAATTTACGCCCTGGCCAGAGGAACTGGCACAGCGTTATCGCGACAAGGGATACTGGGTCGGCCTGCCGTT
AACGGATGCCTGGGAACGTCATCTGGCGACACAGCCCGATGCGGTTGCGGTCGTGTGCGGCGAACGTCAGTGGAGCTATC
GGGAGCTGGATCAACAGTCTTCCGCGCTGGCATCGCGCTTGACGCAAAACGGCCTGCGCGGTGGTGACACTGCTCTGGTG
CAGTTGCCGAACGTGGCCGAATTTTACCTGACCTTTTTTGCGTTGCTGAAAATGGGCGTCGCGCCGGTTAACGCGCTGTT
CAGCCATAACAAGCTGGAGCTGCTGTCGTACGCTACGCAGATCGAGCCGCGTTTGCTGATCGCATCGGCCGAACATCCGC
TGTTTGGCAACGGTGAGTTTCTGGATCGGCTACAAACGCAGGTTCCTAGCCTGCAAACAGTGGTGATGCTCGGCGACAGC
CCACTGGGGCACAGTCTGACGGACTGGCTGCAACCGCACGCTTCGGTCAGTAAGTATCAGCCTTCCGCGTCGGGGCAGGT
CGCCTTCTTTCAGCTTTCTGGCGGCAGTACCGGCACGCCGAAGCTAATTCCCCGTACCCATGATGATTACTACTACAGCG
TGCGCCGCAGCGTGGAAATCTGTGAGCTGACGCCACAAACCCGCTATCTGTGTGCGTTGCCGGCACCGCATAACTTCCCG
TTAAGTTCACCCGGTTCACTGGGCGTATTTTACGCTGGTGGACGGGTCGTGTTGGCGTCAGATCCGGGGGCGATGACTTG
TTTCCCGCTGATCGAACGTCATCAGATCGATATAACTTCGCTGGTGCCGCCCGCTGCCGCGCTGTGGATCCAAGCGGCAG
AACAGTTTGGTGAAGCGCTGCGTAGCCTGAAGATCTTGCAGGTGGGCGGGGCGAAGCTGAGTGAAACCGTCGCACGTCGT
ATTCCGGCGGTGCTGGGCTGTCAGTTGCAACAGGTGTTGGGGATGGCGGAAGGGCTGGTGAACTACACCCGACTGGACGA
CAGCGACGAGCACATCTTTACCACGCAGGGCTACCCGATGAGCCCGGACGATGAAGTGAAGGTGTTGGACAGGGACGGTA
ATCCGCTGCCGAGGGGTGAGGCGGGGCTATTGGCGACGCGTGGCCCGTATACCTTCCGTGGCTATTACCGCAGCCCGGAG
CACAATGCTCGCGCTTTCGACCGTGACGGTTTCTACCACTCGGGCGATGTGGTGCAGATGACCGAAGAGGGCTACTTGCG
TGTGGTCGGGCGAGAAAAAGATCAGATTAACCGCGGCGGTGAAAAGATCGCTGCCGAAGAGATCGAAAATCTGCTGCTTA
AACATGACGGCATTCTCCATGCCGCGCTGGTGTCCATGCCCGATCCGGTCATGGGCGAAAAAAGCTGTGCTTTTCTGGTG
GTCAGCGATTCCTCACTGAAAGCCATCACGTTAAGAAAATATCTTCGCAATCAGGGTATTGCCGAATTCAAACTGCCGGA
CCGCTTTGAGATGATCGACACCTTGCCCGTCACACCGGTCGGCAAGATTGATAAGAAATCACTCCGTCAGCGCATCCAGA
CCCAACTCAGCATTCAAAATAAATAA

Protein sequence : 
MSIEFTPWPEELAQRYRDKGYWVGLPLTDAWERHLATQPDAVAVVCGERQWSYRELDQQSSALASRLTQNGLRGGDTALV
QLPNVAEFYLTFFALLKMGVAPVNALFSHNKLELLSYATQIEPRLLIASAEHPLFGNGEFLDRLQTQVPSLQTVVMLGDS
PLGHSLTDWLQPHASVSKYQPSASGQVAFFQLSGGSTGTPKLIPRTHDDYYYSVRRSVEICELTPQTRYLCALPAPHNFP
LSSPGSLGVFYAGGRVVLASDPGAMTCFPLIERHQIDITSLVPPAAALWIQAAEQFGEALRSLKILQVGGAKLSETVARR
IPAVLGCQLQQVLGMAEGLVNYTRLDDSDEHIFTTQGYPMSPDDEVKVLDRDGNPLPRGEAGLLATRGPYTFRGYYRSPE
HNARAFDRDGFYHSGDVVQMTEEGYLRVVGREKDQINRGGEKIAAEEIENLLLKHDGILHAALVSMPDPVMGEKSCAFLV
VSDSSLKAITLRKYLRNQGIAEFKLPDRFEMIDTLPVTPVGKIDKKSLRQRIQTQLSIQNK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-90 43
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 6e-90 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-90 43
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-90 43
ybtE CAA21394.1 - Virulence HPI Protein 6e-90 43
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-90 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 8e-90 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-89 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-89 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_048596.1 enterobactin synthase subunit E VFG0932 Protein 2e-168 65
entE YP_048596.1 enterobactin synthase subunit E VFG0168 Protein 7e-111 46
entE YP_048596.1 enterobactin synthase subunit E VFG1817 Protein 9e-89 41