Gene Information

Name : hrpI (ECA2085)
Accession : YP_050180.1
Strain : Pectobacterium atrosepticum SCRI1043
Genome accession: NC_004547
Putative virulence/resistance : Virulence
Product : type III secretion system protein
Function : -
COG functional category : U : Intracellular trafficking, secretion and vesicular transport
COG ID : COG4789
EC number : -
Position : 2372933 - 2375035 bp
Length : 2103 bp
Strand : -
Note : Similar to Erwinia amylovora harpin secretion protein HrpI SWALL:HRPI_ERWAM (SWALL:P35654) (697 aa) fasta scores: E(): 2e-193, 76.35% id in 702 aa, and to Pantoea agglomerans HrcV protein HrcV SWALL:Q937I5 (EMBL:X99768) (719 aa) fasta scores: E(): 2.2e-19

DNA sequence :
ATGAATCTGCTGATTATCTGGCTAAATCGTATTGCGCTGAGTGCAATGCAGCGCTCGGAGGTGGTGGGCGCGGTGATCGT
CATGTCTATCGTTTTCATGATGATCATCCCGCTGCCCACCGGACTGATCGATGTGCTGATCGCGCTTAATATCTGTGCCT
CCTCTCTGCTAATCGTGCTGGCGATGTACCTGCCTAAACCACTGGCTTTCTCGACGTTTCCGGCGGTGCTGCTGCTAACC
ACCATGTTCCGGCTGGCGATCTCCATTTCCACCACGCGCCAAATTCTGCTCCAGCAGGACGGCGGCCATATCGTTGAGGC
TTTCGGTAACTACGTGGTGGGCGGGAATCTGGCGGTCGGTCTGGTTATTTTCCTGATTCTGACGGTAGTGAATTTTATGG
TCATCACCAAAGGCTCTGAACGTGTGGCCGAGGTGGCGGCACGCTTCACGCTCGATGCGATGCCAGGTAAACAGATGTCC
ATCGACAGCGATCTCCGCGCTGGGTTAATCGAAGCCCATCAGGCACGGCAGCGGCGGGAAAATCTGGCGAAAGAAAGCCA
GCTTTTCGGGGCAATGGATGGCGCGATGAAGTTCGTTAAAGGCGATGCGATTGCGTCGCTGGTTATCGTGTTCATTAACA
TGATCGGCGGCTTTGCCATCGGCGTGTTGCAACACAATATGGCCGCGTCCGATGCCATGCACGTCTACTCGGTATTGACC
ATCGGCGATGGGCTGATCGCCCAGATTCCGGCACTGCTGATTTCACTGACCGCCGGGATGATCATTACTCGCGTTTCGGC
CGACGGGCAAAAAGTGGATGCCAATATTGGTCGGGAAATAGCGGAGCAGCTCACCAGCCAGCCCAAGGCGTGGATCATCT
CCTCCATCGGCATGTTTGGATTTGCACTGCTGCCGGGGATGCCAACGTTGGTGTTTGTCGCCATCAGTTTGTCCTCGCTC
GGCAGCGGTCTGTTTCAGCTCTGGCGCATCAAACAACAAGGCCAGTTGGATGCCAACCAATTGGAAGCGGACAACATGCC
TGCCGAACAGAACGGTTATCAAGATCTACGGCGCTTCAACCCCACGCGTGCCTACCTGCTGCTGTTTCATCCGGTCTGGC
AAGGGCAGCCGACGGCGACGGTGTTGGTGCAGAATATCCGTCGCCTGCGTAATCGGCTGGTCTACCGCTTTGGCTTTACG
CTGCCGTCCTTTGATATCGAATTCAGCGACCGTCTGGCGGAAGATGAATTCCAGTTTTGTGTCTATGAAATTCCCTACGT
TAAAGCCACATTTGTCACCGACCAGTTGGCGGTTGCCAATGGAGCCATCGAACAGGTTGACGCGACGATCGCCACGCCAG
GCCACACGTTACGGGATGAAAGCCAGTGGCTATGGCTGCCGCTGGCGCATGTCGCCCAGCAGCCGGACGACATACCGCGC
TGGACATCAGATGAGCTGATTCTGGCTCGCATGGAACAGGCCATTCACCGCACAGGTTCACAGTTCATCGGCTTACAGGA
AACCAAATCTATTCTGGCCTGGCTGGAGAGTGAACAGCCGGAGTTGGCGCAGGAGCTACAGCGCATCATGCCGCTTTCGC
GTTTTGCCAGCGTACTGCAACGGCTGGCTTCCGAACGTGTACCGCTGCGGTCGGTGCGCCCTATCGCCGAAGCGCTGATT
GAAGTCGGCCAACACGAACGGGACACGCTGGCGTTGACCGACTACGTGCGTCTGGCGCTCAAATCACAAATCTGCCACCA
GTACAGCGATGAGAACAACCTCGCGGTCTGGCTGCTGACGCCGGAAAGCGAAGAGCTGCTGCGCGATGCGCTACGCCAGA
CGCAGAACGAAACCTTCTTCGCCCTGACGCAAGACTACGCCTCAACGCTGCTCAGCCAATTGCGGCAGGCGTTTCCACCG
TTCTCGTCACAGAGCAGTCTGGTGCTGGTAGCACAGGATTTACGCAGCCCGCTGCGCACGCTGTTACAGGACGAATTTCA
CCACGTCCCCGTGCTGTCCTTCACCGAACTGGAATCCACCCTGTCGATCAACGTCATTGGACGCCTTGATCTTTACGACT
CCCCTGATCCCTTTAGCGCATAG

Protein sequence :
MNLLIIWLNRIALSAMQRSEVVGAVIVMSIVFMMIIPLPTGLIDVLIALNICASSLLIVLAMYLPKPLAFSTFPAVLLLT
TMFRLAISISTTRQILLQQDGGHIVEAFGNYVVGGNLAVGLVIFLILTVVNFMVITKGSERVAEVAARFTLDAMPGKQMS
IDSDLRAGLIEAHQARQRRENLAKESQLFGAMDGAMKFVKGDAIASLVIVFINMIGGFAIGVLQHNMAASDAMHVYSVLT
IGDGLIAQIPALLISLTAGMIITRVSADGQKVDANIGREIAEQLTSQPKAWIISSIGMFGFALLPGMPTLVFVAISLSSL
GSGLFQLWRIKQQGQLDANQLEADNMPAEQNGYQDLRRFNPTRAYLLLFHPVWQGQPTATVLVQNIRRLRNRLVYRFGFT
LPSFDIEFSDRLAEDEFQFCVYEIPYVKATFVTDQLAVANGAIEQVDATIATPGHTLRDESQWLWLPLAHVAQQPDDIPR
WTSDELILARMEQAIHRTGSQFIGLQETKSILAWLESEQPELAQELQRIMPLSRFASVLQRLASERVPLRSVRPIAEALI
EVGQHERDTLALTDYVRLALKSQICHQYSDENNLAVWLLTPESEELLRDALRQTQNETFFALTQDYASTLLSQLRQAFPP
FSSQSSLVLVAQDLRSPLRTLLQDEFHHVPVLSFTELESTLSINVIGRLDLYDSPDPFSA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcV AAB05999.2 HrcV Virulence Hrp PAI Protein 0.0 78
hrcV AAT96256.1 HrcV Virulence S-PAI Protein 0.0 69
hrcV ABA47273.1 HrcV Virulence S-PAI Protein 0.0 69
hrcV AAT96297.1 HrcV Virulence S-PAI Protein 0.0 68
hrcV AAT96337.1 HrcV Virulence S-PAI Protein 0.0 68
hrpI AAA03015.1 HrpI Virulence Hrp PAI Protein 0.0 65
hrcV ABQ88352.1 HrcV Virulence Hrp PAI Protein 0.0 65
hrcV NP_791229.1 type III secretion protein HrcV Virulence Hrp PAI Protein 0.0 65
hrcV AAG33877.1 HrcV Virulence Hrp PAI Protein 0.0 65
hrcV AAT96139.1 HrcV Virulence T-PAI Protein 0.0 64
hrcV AAT96193.1 HrcV Virulence T-PAI Protein 0.0 64
hrcV AAR25631.1 type III secretion protein HrcV Not tested Not named Protein 0.0 63
invA AAS66860.1 InvA Not tested SSR-2 Protein 1e-99 41
YPO0266 YP_002345347.1 secretion system apparatus protein SsaV Virulence Not named Protein 2e-102 41
lssD AAO18050.1 LssD Virulence TTSS locus Protein 7e-120 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrpI YP_050180.1 type III secretion system protein VFG0064 Protein 5e-128 44
hrpI YP_050180.1 type III secretion system protein VFG0384 Protein 4e-119 41
hrpI YP_050180.1 type III secretion system protein VFG0198 Protein 2e-108 41