Gene Information

Name : hrcN (ECA2083)
Accession : YP_050178.1
Strain : Pectobacterium atrosepticum SCRI1043
Genome accession: NC_004547
Putative virulence/resistance : Virulence
Product : type III secretion system protein
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 2370621 - 2371991 bp
Length : 1371 bp
Strand : -
Note : Similar to Erwinia amylovora HrcN SWALL:Q46642 (EMBL:L25828) (454 aa) fasta scores: E(): 3.9e-124, 78.7% id in 446 aa, and to Pantoea agglomerans HrcN protein HrcN SWALL:Q937I3 (EMBL:X99768) (454 aa) fasta scores: E(): 4.7e-121, 76.4% id in 445 aa

DNA sequence :
GTGATGCAGACACAATCTTCTTCTTTTCCGCTGCTCGATCAGTGGGTCGCCCGACAGCGCCAGCATCTGGCGGGTTACGC
GCCGGTGGAGAAAAAAGGCCGCGTCATGGCCGTCAGCGGTATTTTGCTGGAGTGCAGCTTGCCGCAGGCACGTATTGGCG
ATTTGTGCTGGGTGGCGCGTCAGGATGACAGCCAAATGATGGCCGAAATCGTTGGATTCAGCCCGGACAATACTTTCCTC
TCCGCACTCGGTGCGCTGGACGGCATTGCACAAGGTGCGACCGTGACGCCGCTGTATCAGCCGCACCGTATTCAGGTGTC
AGAACGTTTGCTGGGCAGCGTATTGGACGGATTTGGTCGGGCATTGGAAGACGGCGGCGAGAGCGCCTTTGTCGAACCGG
GTCAGGTCACGGGGCGCACGCAGCCGGTGCTGGGCGATGCCCCGCCGCCGACCTCCAGACCGCGCATCAGCCAGCCGCTC
CCCACCGGATTACGCGCCGTCGATGGCCTGCTGACCATCGGTCAGGGTCAGCGCGTCGGTATTTTCGCCGGTGCGGGCTG
CGGCAAAACCACGCTGCTCGCGGAACTGGCGCGTAATACGCCGTGCGACACCATCGTTTTCGGGCTGATTGGCGAACGTG
GGCGCGAACTACGCGAGTTTCTCGACCACGAACTGGACGACGAGCTGCGTAGCCGCACCGTGTTGGTATGTTCGACTTCC
GACCGTAGCAGTATGGAGCGCGCCCGCGCGGCATTCACTGCCACCGCCATCGCCGAAGCCTACCGCGCAGAAGGCCGTCA
GGTGCTGCTGATTCTTGATTCCCTGACGCGTTTCGCCCGCGCCCAGCGTGAAATCGGTCTGGCACTCGGGGAACCACAGG
GGCGAGGCGGGCTACCGCCGTCGGTCTATACGCTGCTGCCGCGTCTGGTCGAACGTGCCGGACAAACGGAAGACGGTGCC
ATCACCGCGCTCTATTCCGTGCTGATCGAACAGGATTCCATGAACGATCCGGTCGCTGACGAAGTTCGTTCGCTGATCGA
CGGACACATCGTGCTCGCCCGACGTCTGGCGGAACAAGGGCATTATCCGGCTATCGACGTGCTCGCCAGCCTGAGCCGTA
CCATGAGTAACGTCGTGGATACCGATCACACCCGCAATGCGGGCGGCGTACGGCGTCTGATGGCAGCTTACAAACAGGTC
GAGATGCTGATCCGCCTCGGCGAATACCAGCCCGGTCATGACGAATTAACCGATTCGGCAGTCAACGCCCATTCAGAGAT
CACACAGTTTCTGCGCCAGTCGATGCGTGAACCGATGCCTTATGGCGTGATTCAACAACAGCTCGCTGGAGTCAGCCGCT
ATGCCCCATAA

Protein sequence :
MMQTQSSSFPLLDQWVARQRQHLAGYAPVEKKGRVMAVSGILLECSLPQARIGDLCWVARQDDSQMMAEIVGFSPDNTFL
SALGALDGIAQGATVTPLYQPHRIQVSERLLGSVLDGFGRALEDGGESAFVEPGQVTGRTQPVLGDAPPPTSRPRISQPL
PTGLRAVDGLLTIGQGQRVGIFAGAGCGKTTLLAELARNTPCDTIVFGLIGERGRELREFLDHELDDELRSRTVLVCSTS
DRSSMERARAAFTATAIAEAYRAEGRQVLLILDSLTRFARAQREIGLALGEPQGRGGLPPSVYTLLPRLVERAGQTEDGA
ITALYSVLIEQDSMNDPVADEVRSLIDGHIVLARRLAEQGHYPAIDVLASLSRTMSNVVDTDHTRNAGGVRRLMAAYKQV
EMLIRLGEYQPGHDELTDSAVNAHSEITQFLRQSMREPMPYGVIQQQLAGVSRYAP

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-156 79
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 9e-142 70
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-141 70
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-141 70
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-142 69
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 9e-121 69
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-123 68
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-132 68
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-132 68
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-129 67
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 6e-118 64
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 6e-118 64
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-78 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-78 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-78 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-78 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-79 49
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-89 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-84 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-85 48
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-83 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-83 48
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-84 48
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-84 48
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-85 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-85 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-85 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 8e-81 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-77 46
escN AAC38383.1 EscN Virulence LEE Protein 3e-77 46
escN AAC31513.1 L0034 Virulence LEE Protein 2e-77 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-77 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-77 46
escN CAI43874.1 EscN protein Virulence LEE Protein 8e-78 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-77 45
escN AAK26715.1 EscN Virulence LEE Protein 3e-77 45
escN AAL57542.1 EscN Virulence LEE Protein 3e-77 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-77 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-76 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-77 45
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-75 45
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 1e-66 43
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-76 42
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-76 42
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-65 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-65 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-65 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-65 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 4e-65 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-64 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrcN YP_050178.1 type III secretion system protein VFG0192 Protein 4e-95 52
hrcN YP_050178.1 type III secretion system protein VFG0048 Protein 2e-90 51
hrcN YP_050178.1 type III secretion system protein VFG0390 Protein 7e-89 49
hrcN YP_050178.1 type III secretion system protein VFG0515 Protein 1e-78 49
hrcN YP_050178.1 type III secretion system protein VFG2144 Protein 6e-87 46
hrcN YP_050178.1 type III secretion system protein VFG1254 Protein 3e-74 46
hrcN YP_050178.1 type III secretion system protein VFG0730 Protein 2e-77 46
hrcN YP_050178.1 type III secretion system protein VFG0812 Protein 1e-77 46
hrcN YP_050178.1 type III secretion system protein VFG0555 Protein 1e-65 42
hrcN YP_050178.1 type III secretion system protein VFG1008 Protein 2e-63 42
hrcN YP_050178.1 type III secretion system protein VFG1778 Protein 3e-64 42
hrcN YP_050178.1 type III secretion system protein VFG2459 Protein 4e-67 41
hrcN YP_050178.1 type III secretion system protein VFG2331 Protein 9e-71 41