Gene Information

Name : fliI (blr6885)
Accession : NP_773525.2
Strain : Bradyrhizobium japonicum USDA 110
Genome accession: NC_004463
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 7576375 - 7577700 bp
Length : 1326 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
TTGAACGCTCTTCGGCAACTCGAGTGGGCGCTGCTGGAGCTTCAGCAGAGCACTCCCCTGGCAAGCGTCAGCGGCGCGAT
CTCCGAGATCGCGCCGACGCATTTCCGTGTCTCCGGCCTGTCGCGCTTCGTCAGGCTCGGTGAACTCATCGGCGTCAACT
CCGGCGGCAAGCCCCAGATCGGCGAGGTGGTGCGGATCGACAGCGAGGGCATCATCGCCAAGCCGTTCGACCGGCAATTT
GCCGGCGGTCTCGGTTCCGTCGCCTACCGCATGCCGCCATTGTCTTTCGCGCCCGATCCGAGCTGGAAGGGCCGGGTCAT
CAACGCGCTCGGCGCCCCGCTGGACGGACAAGGCCCGCTGACGCCGGGATCGCGCGCCGTCTCGGCGGAGGCCGAGGCGC
CCTCGGCCATGAAGCGCGCGCGCGTCCACAAGCCGCTGCGCACCGGCGTGCGCGTCATCGACCTGTTCGCCCCGATCTGC
GCCGGCCAGCGCGTCGGCATCTTTGCCGGCTCCGGCGTCGGCAAATCGACGTTGCTCGCGATGCTTGCCCGCAGCCAGGG
CTTCGACACCGTCGTGCTGGCGCTCGTCGGCGAGCGCGGCCGCGAAGTGCGCGAATTCATCGAGGACGTGCTGGGCGCCA
ATCGCAATCGTGCGGTCACCATCGTATCGACGGGCGACGAGAGCCCGATGATGCGGCGGCTGGCGCCGAAGACGGCCATG
GCGGTAGCCGAATATTTCCGCGATCGGGGCGAATCGGTTCTGCTTGTGGTCGATTCGATCACCCGTTTCGCCCACGCCGC
CCGCGAGGTCGCGCTCGCCGCCGGCGAGCCCGCGGTCGCGCGCGGCTACGCGCCGACGGTCTTCACGGACCTGCCGCGCC
TTCTGGAGCGCGCCGGCCCCGGCGAGGAGGGATCCGGAACGATCACCGGGATTTTCTCCGTGCTGGTCGACGGCGACGAT
CACAACGAGCCGATCGCCGACACCATTCGCAGCACGCTCGACGGGCACATCGTGCTCTCCAGGCACATCGCCGACCAGGC
GCGCTATCCGGCCGTGGACGTTCTGGCCTCGGTCTCCCGCCTCGCCCATAACGTCTGGGACCCCGAAGAGCGCGAATTGG
TGAGCAAGCTGCGCACCATGATCGCCAAATACGAGGACACGCGCGATCTTCGCCTGATGGGCGGATACCAGTCGGGACGT
GATTCGGGCCTCGACCAGGCGATCGACCTGGTTCCGAGAATCTACAGCGCAATGCGGCAAGACGCATCGGCTCAGCCGAG
CGCAGATCCGTTCCGCGAGCTGAGGGACATGCTCAAGGGCGAGTAG

Protein sequence :
MNALRQLEWALLELQQSTPLASVSGAISEIAPTHFRVSGLSRFVRLGELIGVNSGGKPQIGEVVRIDSEGIIAKPFDRQF
AGGLGSVAYRMPPLSFAPDPSWKGRVINALGAPLDGQGPLTPGSRAVSAEAEAPSAMKRARVHKPLRTGVRVIDLFAPIC
AGQRVGIFAGSGVGKSTLLAMLARSQGFDTVVLALVGERGREVREFIEDVLGANRNRAVTIVSTGDESPMMRRLAPKTAM
AVAEYFRDRGESVLLVVDSITRFAHAAREVALAAGEPAVARGYAPTVFTDLPRLLERAGPGEEGSGTITGIFSVLVDGDD
HNEPIADTIRSTLDGHIVLSRHIADQARYPAVDVLASVSRLAHNVWDPEERELVSKLRTMIAKYEDTRDLRLMGGYQSGR
DSGLDQAIDLVPRIYSAMRQDASAQPSADPFRELRDMLKGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 3e-64 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-70 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-70 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-70 49
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-58 47
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-55 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 9e-56 46
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-66 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 7e-66 45
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-66 45
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-66 45
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-66 45
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-71 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-55 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 9e-55 45
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-70 44
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 6e-70 44
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 6e-70 44
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-70 44
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-70 44
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 7e-70 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-57 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 7e-57 44
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-55 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-56 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-52 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 3e-52 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-52 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 3e-52 42
escN AAC31513.1 L0034 Virulence LEE Protein 1e-61 41
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-61 41
escN AAC38383.1 EscN Virulence LEE Protein 8e-62 41
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-61 41
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-61 41
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 7e-52 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI NP_773525.2 flagellum-specific ATP synthase VFG0192 Protein 1e-66 50
fliI NP_773525.2 flagellum-specific ATP synthase VFG0048 Protein 5e-70 50
fliI NP_773525.2 flagellum-specific ATP synthase VFG2144 Protein 5e-69 47
fliI NP_773525.2 flagellum-specific ATP synthase VFG0515 Protein 2e-66 45
fliI NP_773525.2 flagellum-specific ATP synthase VFG2331 Protein 2e-66 44
fliI NP_773525.2 flagellum-specific ATP synthase VFG1254 Protein 3e-67 44
fliI NP_773525.2 flagellum-specific ATP synthase VFG0555 Protein 1e-52 42
fliI NP_773525.2 flagellum-specific ATP synthase VFG0730 Protein 5e-62 41
fliI NP_773525.2 flagellum-specific ATP synthase VFG0812 Protein 6e-62 41