Gene Information

Name : fliI (blr2201)
Accession : NP_768841.1
Strain : Bradyrhizobium japonicum USDA 110
Genome accession: NC_004463
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 2377131 - 2378456 bp
Length : 1326 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGAAGGCTCTGGCCGAACAGATCGGCGATATCGACGGCGTCAATATCTATGGCCGTGTGGTCGGTGTGCGCGGCCTGAT
GGTCGAGGTGGCCGGCCCGATCCATGCGATGTCGGTCGGCGCGCGGCTGGTGATCGAAACCGGCGCCAACCGTTCCATCC
CCTGCGAGGTGATCGGCTTCTCCGGCAACAATGCTGTCGTGATGCCGTTCGCCGGTCTCGACGGCGTGCGCCGCGGCTGC
AAGGCGGTCATCGCCAATGCCGCCAATCAGGTGCGGCCCTCGACGGCCTGGCTCGGCCGCGTCGTCAATGCGCTGGGCGA
GCCCATCGACGGCAAGGGGCCGTTGCCGCAGGGGTCCTCGCCGATGCCGTTCCGCAATACGCCGCCGCCGGCGCATTCGC
GCAAGCGCGTAGGTAGCCCGCTCGATCTCGGCGTGCGCGCGATGAACACGTTCCTCACCTGCTGCCGCGGCCAGCGCATG
GGCATCTTCGCGGGCTCCGGCGTCGGCAAATCGGTGCTGCTCTCGATGCTGGCACGCAACGTGGACGCCGCCGTCAGCGT
CATCGGGCTGATCGGCGAGCGCGGCCGCGAGGTTCAGGAGTTCTTGCAGGATGATCTCGGCGAGGAGGGCCTGGCGCGCT
CCGTCGTCGTGGTGGCGACCTCCGACGAACCGGCGCTGATGCGCCGCCAGGCCGCCTATCTGACGCTCGCGGTCGCCGAA
TATTTTCGCGACGAGGATAAGGACGTCCTCTGCCTGATGGACTCGGTGACGCGCTTTGCCATGGCCCAGCGCGAGATCGG
CCTGTCCGCTGGCGAGCCGCCGACCGCCAAGGGCTATACGCCGACCGTGTTCACCGAGTTGCCGAAGCTGTTGGAGCGTG
CCGGACCGGGCCTGGGGGAGGGCGCGATCACCGCGATCTTCACGGTGCTGGTCGACGGCGACGACCACAACGAGCCGATC
GCCGACGCCGTCCGCGGCATCCTCGACGGCCACATCGTGATGCAGCGCTCGATCGCCGAGCGCGGCCGCTACCCCGCCAT
CAACATCCTCAAATCCGTCTCCCGCACCATGCCGAAATCGGCCGATCCGGAGTTCTGGCCGACCATCCAGAAGGCGCGCC
AAGTGATGGCGACCTATGCCGACATGGAGGAATTGATCCGGCTTGGCGCCTACCGGGCTGGCTCCAGCCCCGAGGTCGAC
GAGGCGATCCGGCTGCACGAACCCCTGGAAGGCTTCCTGCGCCAGCGCAAGGACGAAAATGCCTCATTGGCGGACGGTTA
CCGCCAGTTGGCGCAAATCCTCGGTAATTTGGAAACGGAACGCTAA

Protein sequence :
MKALAEQIGDIDGVNIYGRVVGVRGLMVEVAGPIHAMSVGARLVIETGANRSIPCEVIGFSGNNAVVMPFAGLDGVRRGC
KAVIANAANQVRPSTAWLGRVVNALGEPIDGKGPLPQGSSPMPFRNTPPPAHSRKRVGSPLDLGVRAMNTFLTCCRGQRM
GIFAGSGVGKSVLLSMLARNVDAAVSVIGLIGERGREVQEFLQDDLGEEGLARSVVVVATSDEPALMRRQAAYLTLAVAE
YFRDEDKDVLCLMDSVTRFAMAQREIGLSAGEPPTAKGYTPTVFTELPKLLERAGPGLGEGAITAIFTVLVDGDDHNEPI
ADAVRGILDGHIVMQRSIAERGRYPAINILKSVSRTMPKSADPEFWPTIQKARQVMATYADMEELIRLGAYRAGSSPEVD
EAIRLHEPLEGFLRQRKDENASLADGYRQLAQILGNLETER

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 5e-84 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-72 47
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-79 46
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-79 46
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-80 46
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-80 46
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-80 46
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-72 46
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-71 46
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-71 46
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-72 46
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 3e-72 46
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-79 44
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-79 44
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-79 44
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-80 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 9e-72 43
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 9e-72 43
unnamed AAL06369.1 EscN Virulence LEE Protein 5e-72 43
escN YP_003236088.1 translocator EscN Virulence LEE Protein 4e-72 43
escN AAC31513.1 L0034 Virulence LEE Protein 7e-72 43
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 7e-72 43
escN AAC38383.1 EscN Virulence LEE Protein 2e-71 43
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-71 43
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-71 43
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-71 43
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-71 43
escN AAK26715.1 EscN Virulence LEE Protein 3e-71 43
escN AAL57542.1 EscN Virulence LEE Protein 3e-71 43
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-71 43
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-72 43
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-59 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-59 42
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-59 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 5e-59 42
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 6e-69 41
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 5e-61 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI NP_768841.1 flagellum-specific ATP synthase VFG2499 Protein 3e-80 48
fliI NP_768841.1 flagellum-specific ATP synthase VFG1254 Protein 6e-83 47
fliI NP_768841.1 flagellum-specific ATP synthase VFG2144 Protein 3e-88 46
fliI NP_768841.1 flagellum-specific ATP synthase VFG0515 Protein 1e-72 46
fliI NP_768841.1 flagellum-specific ATP synthase VFG0048 Protein 1e-85 45
fliI NP_768841.1 flagellum-specific ATP synthase VFG0192 Protein 3e-82 45
fliI NP_768841.1 flagellum-specific ATP synthase VFG2331 Protein 2e-80 44
fliI NP_768841.1 flagellum-specific ATP synthase VFG0390 Protein 1e-83 44
fliI NP_768841.1 flagellum-specific ATP synthase VFG0812 Protein 4e-72 43
fliI NP_768841.1 flagellum-specific ATP synthase VFG0730 Protein 1e-71 43
fliI NP_768841.1 flagellum-specific ATP synthase VFG0555 Protein 2e-59 42