Gene Information

Name : c3655 (c3655)
Accession : NP_755530.1
Strain : Escherichia coli CFT073
Genome accession: NC_004431
Putative virulence/resistance : Virulence
Product : antigen 43
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG3468
EC number : -
Position : 3491808 - 3494936 bp
Length : 3129 bp
Strand : +
Note : AG43; Sap; Escherichia coli K-12 ortholog: b2000; Escherichia coli O157:H7 ortholog: z1651

DNA sequence :
ATGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATTACGGGCGCTTTCGTGGTTGCCTCCGA
ACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCGGTCACGCCACTCCCGGTGCTGT
CTGCTGACATCGTTGTGCACCCGGGTGAAACAGTGAATGGCGGAACACTGGTAAACCATGACAACCAGTTTGTATCCGGA
ACAGCTAATGGCGTGACTGTCAGTACCGGGCTTGAGCTGGGGCCGGACAGTGACGAAAACACCGGCGGGCAATGGATAAA
AGCGGGTGGCACAGGCAGAAACACCACTGTCACCGCAAATGGTCGTCAGATTGTGCAGGCAGGAGGAACTGCCAGTGATA
CGGTTATTCGTGATGGCGGAGGGCAGAGCCTTAACGGACTGGCGGTGAACACCACGCTGGATAACAGAGGTGAGCAGTGG
GTACACGGGGGAGGGAAAGCTGCCGGTACAATTATTAACCAGGATGGTTACCAGACCATAAAACATGGCGGACTGGCAAC
CGGAACCATCGTCAACACCGGTGCAGAAGGTGGTCCGGAGTCTGAAAATGTGTCCAGCGGTCAGATGGTCGGAGGGACGG
CTGAATCCACCACCATCAATAAAAATGGCCGGCAGGTTATCTGGTCTTCGGGGATGGCACGGGACACCCTCATTTACGCC
GGTGGTGACCAGACGGTACACGGAGAGGCACATAACACCCGACTGGAGGGGGGTAACCAGTATGTACACAACGGTGGCAC
GGCAACAGAGACGCTGATAAACCGTGATGGCTGGCAGGTGATTAAGGAAGGAGGAACTGCCGCGCATACCACCATCAACC
AGAAAGGAAAGCTGCAGGTGAATGCCGGCGGTAAAGCGTCTGATGTCACCCAGAACACGGGCGGTGCACTGGTTACCAGT
ACAGCTGCAACCGTCACCGGCACAAACCGCCTGGGAGCATTCTCCGTTGTGGCGGGTAAGGCTGATAATGTCGTACTGGA
AAATGGCGGACGTCTGGATGTGCTGAGCGGACACACAGCCACTAATACCCGTGTGGATGATGGCGGAACGCTGGATATCC
GCAACGGTGGTGCCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGT
ACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCAC
GCTGAACGCCGGTGATACGGCCACGGATACCACGGTAAATGGCGGACTGTTCACCGCCAGGGGCGGCACACTGGCGGGCA
CCACCACGCTGAATAACGGCGCCATACTTACCCTTTCCGGGAAGACGGTGAACAACGATACCCTGACCATCCGTGAAGGC
GATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAA
CACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCA
TTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTC
GCCTCCGATGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGAT
TCATTTCACCTCCTCACGCACAGGGACATTTGTGCCGGCGACTCTGAAAGTGAAAAACCTGAACGGGCAGAATGGCACCA
TCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGACTGGTCATTGACGGCGGCAGGGCAACCGGAAAA
ACCATCCTGAACCTGGTGAACGCCGGCAACAGTGCGTCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGC
CATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACAGGCTGCAGGCCGGGGCCTTTAACTACTCCCTCA
ACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGTCCCCCTGTATGCCTCCATGCTG
ACACAGGCAATGGACTATGACCGGATTCTGGCAGGCTCACGCAGCCATCAGACCGGTGTAAACGGTGAAAATAACAGCGT
CCGTCTCAGCATTCAGGGCGGCCATCTCGGTCACGATAACAACGGCGGTATTGCCCGTGGGGCCACGCCGGAAAGCAGCG
GCAGCTATGGCTTCGTCCGCCTGGAGGGTGACCTGCTGAGAACAGATGTTGCCGGTATGTCTGTGACCGCAGGGATATAT
GGTGCTGCAGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAG
CCTGGGCGGATATATGAACCTGACACACACCTCCTCCGGCCTGTGGGCTGACATAGTGGCACAGGGAACCCGCCACAGTA
TGAAAGCGTCATCGGGCAATAACGACTTCCGCGCACGGGGCCGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTC
AGTATCACTGACAACCTGATGCTGGAGCCACGGCTGCAGTACACCTGGCAGGGGCTTTCGCTGGATGACGGTAAGGACAA
CGCCGGTTATGTGAAGTTCGGGCATGGCAGTGCACAACATGTGCGTGCCGGTTTCCGTCTGGGCAGCCACAACGATATGA
CCTTTGGTGAAGGCACCTCATCCCGTGCCCCCCTGCGTGACAGTGCAAAACACAGTGTGCGTGAACTGCCGGTGAACTGG
TGGGTACAGCCTTCCGTTATCCGCACCTTCAGCTCCCGGGGTGATATGCGTGTGGGGACCTCCACTGCAGGCAGCGGGAT
GACGTTCTCTCCCTCACAGAATGGCACATCACTGGACCTGCAGGCCGGACTGGAAGCCCGTGTCCGGGAAAATATCACCC
TGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTG
ACCTTCTGA

Protein sequence :
MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAAVTPLPVLSADIVVHPGETVNGGTLVNHDNQFVSG
TANGVTVSTGLELGPDSDENTGGQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYA
GGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTS
TAATVTGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDIRNGGAATTVSMGNGGVLLADSGAAVSG
TRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKTVNNDTLTIREG
DALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTL
ASDATWNIPDNATVQSVVDDLSHAGQIHFTSSRTGTFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGK
TILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASML
TQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTDVAGMSVTAGIY
GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASSGNNDFRARGRGWLGSLETGLPF
SITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNW
WVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNV
TF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SF2991 NP_708765.1 outer membrane fluffing protein Not tested SHI-1 Protein 0.0 96
unnamed CAD66200.1 hypothetical protein Virulence PAI III 536 Protein 0.0 96
sap AAK00474.1 Sap Not tested SHI-1 Protein 0.0 96
aidA ADD91708.1 AidA Not tested PAI-I AL862 Protein 0.0 88
flu CAI43838.1 antigen 43 Not tested LEE Protein 0.0 87
Z1211 NP_286746.1 adhesin Not tested TAI Protein 0.0 58
Z1651 NP_287154.1 adhesin Not tested TAI Protein 0.0 58
unnamed AAL08472.1 putative autotransporter Not tested SRL Protein 0.0 57
unnamed CAE85197.1 antigen43 protein orthologue Not tested PAI V 536 Protein 0.0 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
c3655 NP_755530.1 antigen 43 VFG1675 Protein 0.0 96
c3655 NP_755530.1 antigen 43 VFG0655 Protein 0.0 96
c3655 NP_755530.1 antigen 43 VFG1063 Protein 0.0 57