Gene Information

Name : fliI (c2358)
Accession : NP_754250.1
Strain : Escherichia coli CFT073
Genome accession: NC_004431
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 2168322 - 2169695 bp
Length : 1374 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTACGTCG
CTACGGGCGATTAACCCGCGCTACCGGGCTGGTGCTGGAAGCCACCGGATTACAATTGCCGCTCGGCGCAACCTGTGTCA
TTGAGCGCCAGAACGGCAGCGAAACGCACGAAGTAGAAAGCGAAGTCGTTGGCTTTAACGGTCAACGGCTGTTTTTAATG
CCGCTGGAGGAAGTGGAAGGTGTCCTGCCCGGCGCGCGTGTATATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGG
CAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGTGTTCTGGACGGTAGCGGTAAACCGCTCGATGGCCTGCCCTTCC
CCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGAC
ACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTCGGACGCGGGCAGCGTATGGGGCTGTTTGCCGGGTCCGGCGTTGG
GAAAAGTGTGCTGCTGGGGATGATGGCCCGTTACACCCGCGCCGATGTCATTGTCGTGGGTTTGATTGGTGAACGAGGGC
GCGAAGTAAAAGATTTTATTGAGAATATCCTCGGTGCCGAAGGACGTGCACGCTCCGTGGTGATTGCCGCTCCGGCGGAT
GTTTCTCCGCTCCTGCGAATGCAGGGTGCCGCCTATGCCACGCGCATTGCCGAAGATTTTCGCGATCGTGGTCAGCATGT
GTTGCTGATTATGGACTCCCTCACCCGCTACGCGATGGCCCAGCGTGAAATTGCGCTGGCGATTGGCGAACCACCTGCCA
CTAAAGGTTATCCACCGTCGGTGTTTGCCAAATTACCGGCACTGGTCGAGCGTGCCGGAAATGGCATTAGCGGCGGCGGC
TCGATTACCGCGTTTTATACCGTGCTCACCGAAGGCGATGACCAGCAGGACCCCATTGCTGACTCCGCGCGGGCAATCCT
CGACGGTCACATTGTGCTGTCTCGCCGACTGGCGGAAGCCGGGCACTATCCGGCTATCGATATTGAAGCGTCGATCAGTC
GCGCAATGACGGCGTTGATCAGTGAGCAACATTACGCGCGAGTGCGCACCTTCAAACAGCTGTTGTCGAGTTTTCAGCGT
AACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGACAAAGCCATCGCCCTGTGGCCGCA
GCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCC
CGACAGTGTCATAA

Protein sequence :
MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGLQLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLM
PLEEVEGVLPGARVYAKNISAEGLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKDFIENILGAEGRARSVVIAAPAD
VSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGG
SITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFKQLLSSFQR
NRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLERIFPTVS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-78 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-69 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 8e-70 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-70 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-69 48
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-82 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-83 47
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-82 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 7e-83 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 7e-83 47
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-81 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-82 47
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-79 46
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-79 46
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-75 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-70 45
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-70 45
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-72 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-72 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-69 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 4e-72 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-72 44
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-70 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-72 43
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-76 43
ECs4568 NP_312595.1 EscN Virulence LEE Protein 7e-71 43
escN YP_003236088.1 translocator EscN Virulence LEE Protein 7e-71 43
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-70 43
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-70 43
escN AAC31513.1 L0034 Virulence LEE Protein 6e-71 43
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-70 43
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 6e-71 43
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-70 43
escN AAC38383.1 EscN Virulence LEE Protein 4e-71 43
escN AAK26715.1 EscN Virulence LEE Protein 1e-70 43
escN NP_290268.1 hypothetical protein Virulence LEE Protein 7e-71 43
escN AAL57542.1 EscN Virulence LEE Protein 1e-70 43
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 6e-72 41
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-72 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-71 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 5e-65 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 5e-65 41
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-70 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI NP_754250.1 flagellum-specific ATP synthase VFG2331 Protein 8e-177 85
fliI NP_754250.1 flagellum-specific ATP synthase VFG2499 Protein 6e-144 70
fliI NP_754250.1 flagellum-specific ATP synthase VFG1254 Protein 2e-120 63
fliI NP_754250.1 flagellum-specific ATP synthase VFG0515 Protein 6e-70 48
fliI NP_754250.1 flagellum-specific ATP synthase VFG0048 Protein 9e-82 46
fliI NP_754250.1 flagellum-specific ATP synthase VFG0192 Protein 2e-75 46
fliI NP_754250.1 flagellum-specific ATP synthase VFG2144 Protein 5e-84 45
fliI NP_754250.1 flagellum-specific ATP synthase VFG0390 Protein 4e-75 45
fliI NP_754250.1 flagellum-specific ATP synthase VFG0730 Protein 3e-71 43
fliI NP_754250.1 flagellum-specific ATP synthase VFG0812 Protein 3e-71 43
fliI NP_754250.1 flagellum-specific ATP synthase VFG1008 Protein 5e-63 41