Gene Information

Name : pykF (c2071)
Accession : NP_753966.1
Strain : Escherichia coli CFT073
Genome accession: NC_004431
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1911623 - 1913251 bp
Length : 1629 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGGCACTCACGTTGGGCTGAGACACAAGCACACATT
CCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACAACCACTTTCGTAATACCGGATTCGCTTTCCGGCA
GTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGC
ACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTT
CTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCG
CTATCCTGCTTGATACCAAAGGTCCAGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCGGGT
CAGTCCTTTACTTTCACTACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTAC
TGACCTGTCTGTTGGCAACACCGTACTGGTTGACGACGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAG
TTATCTGTAAAGTGCTGAACAATGGCGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCCCTGCCA
GCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCG
TAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCG
AAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGT
GTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTTGTTAT
CACTGCGACACAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCA
TCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATG
GCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGTATTAC
CGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCACTGATCGTGGTTGCTACTCAGGGCGGTA
AATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCACCAG
TTAGTACTGAGCAAAGGTGTTGTGCCGCAGCTGGTTAAAGAGATCACATCTACTGATGACTTCTACCGTCTGGGTAAAGA
ACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGATGTCGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTA
CTAACACCGCATCTGTTCACGTCCTGTAA

Protein sequence :
MVSALWTVELNDSKTGTHVGLRHKHTFLCTLFRCHLSLERGTTTFVIPDSLSGSAPRKQVSPILLNLKTKTVMKKTKIVC
TIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAG
QSFTFTTDKSVIGNSEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALP
ALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGIMVARGDLG
VEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIM
ATICERTDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQ
LVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVSGALVPSGTTNTASVHVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 0.0 96
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 0.0 96
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 0.0 96
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 0.0 96

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF NP_753966.1 pyruvate kinase VFG0479 Protein 0.0 96