Gene Information

Name : SF2965 (SF2965)
Accession : NP_708739.1
Strain : Shigella flexneri 301
Genome accession: NC_004337
Putative virulence/resistance : Virulence
Product : superfamily I DNA helicase
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG1112
EC number : -
Position : 3054451 - 3057966 bp
Length : 3516 bp
Strand : +
Note : -

DNA sequence :
ATGGATGAAAATGCTTTAGGGTTTGCCTCATACTGGCGCAACTCGCTTGCGGATGCTGAGTCAGGAAAGGGCAGTTTTAA
ACGGAAAGACGCCCAAAATTTCACTCACTGGCATGGGATAGCGGCGGGACGTCTTGACGAAGCGATTGTCAGTAAATTTT
TTGAGGGAGAAAAAGACGATGTCGAAACGGTCGATGTCATCTTGCGCCCAAAAGTTTATTTCCGGTTACTGCAGCATGGT
AAGGACCGTTCCGCAGGCGCGCCTGATATTGTTACCCCGATAGTGACGCCAGCCTTGCTAAGCCGTGAGGGTTTTTTATA
TCCGACGCCAGCGACCTCCATTCCCAGAGACCTGCTTGAACCTTTGCCAAAAGGAGCATTTTCGATTGGTGAGATTGGGC
AGTATGACAAATACAAGACGACCCATACCACGTTCTCTATCAACTTTGATGACAGCGTTGATAAGACTGCCGAAACGGAT
GAAGAACGGGAAGCACGATATGCCGCCTTGCAGCAGGAGTGGCGTCAATATCTGTATGACTCAGAGAGGCTACTGAAGAG
CGTTGCCGGCGACTGGATTGAAAAACCTGAGCAATATGAACTCGCTGAGCACGGTTATATTGTTAAAACGGCTCAATCTG
GCGGTGCCAGTTCCCATATCCTTTCTCTTTATGATCACCTGCTTGTTTGCAATAAGGATGTGCCGCTCTTCAATCGCTTC
GCCTCGCGAGAGGTTCATGCTGCAGAGTCTTTGCTGGCCCCAGGAGCAAAATTCAGCGACAGGCTTGGACACTCCGGAGA
TAAGTTTCCGCTGGCAAAGGCTCAGCGCGATGCCTTAAGCCATTTTCTGGATGCAAGACATGGCGATATCCTTGCTGTTA
ATGGCCCTCCGGGAACCGGAAAAACCACGCTGGTGCTTTCTATCATCGCCACGCAGTGGGCCAGAGCGGCTCTCGAAAAA
TCTGAGCCTCCGGTTATTATCGCGACTTCAACGAATAACCAGGCTGTAACGAACATTATTGAGGCATTCGGGAAAGACTT
TTCGCAAGGTTCAGGTGCGATGGCCGGGCGATGGTTGCCAGAGCTGAAAAGCTTCGGTGCTTATTTTCCCTCAAGCAGTC
GTAAAGCTGAGGCAGCCAAAAAATATCAAACTGAAGATTTCTTCAACCAGGTTGAGTCAAAAGAGTATGTAGAGGATGCA
CTGCTGTTTTATCTGGAAAAGGCTAAGGCAGCCTTTCCTGGAAAAGAGTGTTCATCCCCTGAAAAGGTCATTGAACTCTT
GCATGGTCAGTTGGCAGCAAAATCTGAGCAACTGATAAGACTGAACGCAACATGGCAAACGTTAAGCCAGATTCGGGCTG
CGCGTGAGCTTATTGCTAATGATATTGAGCAATATCTCGATAATTTAAATAAATTACTTTCCGGACAAGAACAAAAAGTC
ACTCTACTGAAGAGTGCTAAAACGGAATGGAAAAAATATCGCGCCGGTGAATCACTGATCTATTCATTATTTTCCTGGCT
CCCGGCGGTTCGCAATAAGCGACAGTACCAAATACAGCTGTTTCTCGAAGATAAATTAGGCGCGCTGATTGCAGGAAATC
AGTGGTCTGATCCTGAAACTATCGAACGTAATATTGATGGGCTGCTCAATTCCGCTGAGCGCGAGCAAACAACATACCGG
CAGCAGATTGACTCCGCCCATGAAATCGTTCTTAAAGAACAGCAGGCGGTTCAGGAGTGGCAGAGGCTGGCATTTGATTT
AGGGTATGAGGGCGACGAGGAACTGAGCTTCTCACAGGCCGATGAACTGGCTGATACGCAGATTCGCTTCCCTGCATTTT
TACTGACGACTCACTACTGGGAAGGTCGTTGGCTGATGGATATGGCCAGCATTGATGATCTGCAGGACGAGAAGAAGAAA
AAAGGTGCTAAAGGGGTAACCGCCCGTTGGCAACGTCGAATGAAACTCACGCCATGTGTGGTGATGACATGCTATATGCT
GCCCGGTAATATGCAGATAAGTGAGCACAAAGGACAACGTAAATTCGAGAAAAGTTATTTGTATGATTTTGCCGATTTAC
TCATTGTCGATGAAGCCGGGCAGGTGCTTCCTGAAGTGGCTGCTGCCTCGTTTGCATTAGCTAAGAAGGCATTAGTGATT
GGCGATACGGAGCAGATCCCGCCAATATGGAGTATTGCTCCTGCGATTGATGTCGGTAACATGCTGGCGGAAAAAATTCT
GTCTGGCAGTACGCAAGAAGAGATTACCGAGAAATATACGGCAATCGCAGACCTTGGTAAAAGTGCCGCATCTGGCAGCG
TTATGAAAATAGCGCAGTTTGCTTCGCGCTATCAATATGATCCCGAACTGGCTCGTGGTATGTACCTATATGAACACCGC
CGGTGCTACGACAATATTATTGGATACTGTAATACGCTCTGCTATCACGGTAAGTTGTTGCCTAAAAGAGGGCGTGAAGA
GAGCAATTTAATGCCCGCAATGGGGTATCTCCATATTGATGGTAAAGGAGAGCTGGCAAGTAGTGGAAGTCGATATAATT
TGCTTGAGGCTGAAACGATAGCGGTCTGGTTGGCAGAGAACCAGCAAAATATTGAAGCGCATTACGGTAAATCGCTTCAT
GAAGTTGTCGGTATTGTGACGCCTTTTAGCGCTCAGGTATCCACTATCAAACAGGTGCTGGGCAAACAAGATATCAGTAC
AGGCACGAATGAAAAGTCGCTCACAGTGGGCACCGTGCACTCTCTTCAGGGAGCGGAAAGAGCGATTGTGATATTCTCGC
CAGTCTATTCAAAACATGAAGACGGCGGGTTTATTGATAGCGATAACAGCATGCTGAATGTTGCAGTCTCCCGTGCGAAG
GACAGTTTTCTGGTCTTCGGCGATATGGACCTGTTTGAGGTCCAGCCAGCCTCATCGCCACGGGGATTACTGGCAAAATA
CCTCTTTGAGTCAGAGAAGAATGCGCTCTCTTTTGATTATAAAGAGCGTAAGGATTTAAAAACCGCCGGGACCAAAATCT
ACACACTTCATGGTGTGGAGCAACATGATAATTTCCTGAATCAGACATTTGAAAATACCAGTAAACACATCACGATAATT
TCTCCATGGCTGACCTGGCAAAGGCTGGAGCAAACCGGTTTTCTTGATTCCATGATTGCGGCGTGTTCACGTGGAATTAA
CGTCACGATAGTCACTGACAGAAGCTACAACACTGAACATAATGATTTTGAGAAGCGAAAAGAGAAGCAGCAGAACTTTA
AAGCGGCGCTGGAGAAACTGAATGCGCTGGGTATTGCTACAAAGCTGGTAAACCGTGTTCATAGCAAAATTGTTATTGGT
GATGATGGTTTGCTGTGTGTGGGATCGTTCAACTGGTTTAGTGCGACACGGGAAGCGCGATATGAACGATACGATACATC
AATGGTTTATTGCGGTGATAACCTGAAGGGTGAGATTGAGGCTATTTATAATAGTCTTGAGAGGCGTCAGGTTTAG

Protein sequence :
MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGRLDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHG
KDRSAGAPDIVTPIVTPALLSREGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASSHILSLYDHLLVCNKDVPLFNRF
ASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQRDALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEK
SEPPVIIATSTNNQAVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVESKEYVEDA
LLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQIRAARELIANDIEQYLDNLNKLLSGQEQKV
TLLKSAKTEWKKYRAGESLIYSLFSWLPAVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYR
QQIDSAHEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRWLMDMASIDDLQDEKKK
KGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKFEKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVI
GDTEQIPPIWSIAPAIDVGNMLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYEHR
RCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLEAETIAVWLAENQQNIEAHYGKSLH
EVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSLTVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAK
DSFLVFGDMDLFEVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTFENTSKHITII
SPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKEKQQNFKAALEKLNALGIATKLVNRVHSKIVIG
DDGLLCVGSFNWFSATREARYERYDTSMVYCGDNLKGEIEAIYNSLERRQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
S3169 NP_838460.1 superfamily I DNA helicase Not tested SHI-1 Protein 0.0 100
SF2965 NP_708739.1 superfamily I DNA helicase Not tested SHI-1 Protein 0.0 100
unnamed CAD42018.1 hypothetical protein Not tested PAI II 536 Protein 0.0 98
APECO1_3532 YP_854230.1 superfamily I DNA helicase Not tested PAI I APEC-O1 Protein 0.0 98
ORF_2 AAZ04413.1 superfamily I DNA helicase Not tested PAI I APEC-O1 Protein 0.0 98

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
SF2965 NP_708739.1 superfamily I DNA helicase VFG0627 Protein 0.0 100
SF2965 NP_708739.1 superfamily I DNA helicase VFG1537 Protein 0.0 98