Gene Information

Name : cadA_1 (PFL_5892)
Accession : YP_262950.2
Strain : Pseudomonas protegens Pf-5
Genome accession: NC_004129
Putative virulence/resistance : Resistance
Product : cadmium-exporting ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : 3.6.3.3
Position : 6701491 - 6703830 bp
Length : 2340 bp
Strand : -
Note : identified by similarity to GB:AAK48831.1; match to protein family HMM PF00122; match to protein family HMM PF00702; match to protein family HMM TIGR01494; match to protein family HMM TIGR01512; match to protein family HMM TIGR01525

DNA sequence :
ATGAGCGATTCTCTCCACAGCCCGCGCAAAACCGCGGATCAGCAGCCCCAGGCTGATCACGATCACGATCACGATCACGA
TCACGATCACAGCCATGGCCACAGCCATTCCCATGCGCACGCGGGCAAGCTGCAGCCGGTCAAGGCACACGATCATGCCG
CCCATGGCGCTTCCTGCTGCGGTTCCGCCGGCCTGGCGCCGTCCAGGGTCACCCTGAGCGAGACCCCGACCGCCGGCGCG
CGCTTGAGCAGCTTTCGCATCGAAGCCATGGACTGCCCCACCGAACAGACCCTGATCCAGAACAAGCTGGGCAAGTTGGC
CGGGGTCCAGGCGCTGGAGTTCAACCTGATGAACCGCGTGCTGGGGGTGACCCACGACCTGCCGAGCACCGCGCCTATCA
TCGAGGCGGTCAAGTCACTGGGCATGCACGCCGAACCCCTGGAACAGGAGGGTGACCAAGCCGCGCCTGCCGCAGCGCCC
GTGAAGAAGCCCTGGTGGCCGCTGGCCCTGTCCGGTATCGGCGCGCTGGCTGCCGAGGTCATCCATTTCACCAATGCCGC
GCCCGACTGGGTGATTGCCCTGGTGGCCCTGGTGTCGATCCTCAGTGGTGGCCTGACCACCTACAAGAAGGGCTGGATCG
CCCTGAAGAACCGCAACCTCAACATCAATGCCCTGATGAGCATCGCCGTGACCGGCGCCGTGCTGATCGGCCAATGGCCG
GAAGCGGCGATGGTGATGTTCCTGTTCACCGTGGCGGAATTGATCGAGGCCCGTTCCCTGGATCGGGCGCGCAACGCCAT
CAGCGGCCTGATGCAGATGACCCCGGAACGGGTCACGGTGCAGCAGGCAGACGGCAACTGGAGCGAACAGGAAGCGAAAA
ACGTCGAACTTGGCGCACTGGTGCGGGTGCGTCCCGGCGAGCGCATCGGCCTCGACGGCGAGGTCGTGTCCGGCAACTCC
ACCATCGACCAGGCGCCGATCACCGGTGAAAGCCTGCCGGTGGAAAAGACTGTGGGCGACAAGGTTTTCGCCGGCACCAT
CAACCAGGCCGGCTCCCTGGAATACCGAGTGACTGCCGCCGCCAACAACTCGACCCTGGCGCGGATCATCCACGCTGTGG
AACAGGCCCAGGGCGCCCGGGCTCCGACCCAGCGCTTCGTTGACAGCTTCTCGAAAATCTACACCCCGGCGGTATTCGCC
TTCGCCCTGGCGGTGGCGCTGATTCCGCCGCTATTCATGGGCGGCGTCTGGTTCGACTGGATCTACCGCGCCCTGGTGCT
GCTGGTAGTGGCTTGCCCCTGCGCCCTGGTGATCTCCACCCCGGTGAGCATTGTCAGCGGCCTGGCGGCTGCCGCGCGCA
AGGGCATCCTGGTCAAGGGCGGGGTCTACCTGGAAGGCGGCTACAAGCTCGACTACCTGGCCCTGGACAAGACCGGCACC
ATCACCCACGGCAAACCGGTGCAGACCGACTTCCTGTCCCTGGACCCGCTGGCCCAGGACACCGCCCCGGCCCTGGCCGC
AAGCCTGGCAGGGCGTTCCGACCACCCGGTGTCCCTGGCCATTGCCCAGGCGGCTGTGGATAAACAGTTGGCCATGCACC
CTGTGGATAACTTCGAGGCCCTGACCGGCCGAGGCGTACGCGGCGAGATCAATGGCGAGCTGTACCACCTGGGCAACCAT
CGTCTGGTGGAAGAGCTGGGCCTGTGCTCCCCGGCTTTGGAAGAAAAACTCTTCGCCCTGGAGAAGCAGGGCAAGACCGT
GGTCCTGCTGCTGGACCGCAATGGCCCGCTGGCGCTGTTCGCCGTGGCCGATACGGTGAAGGAATCCAGCCGCGAAGCCA
TCCAGCAATTGCATGAGCTGGGGATCAAGACCCTGATGCTCACCGGCGACAACCCGCATACCGCCCAGGCCATCGCGGCC
CAGGTGGGTATCGACCAGGCCCGGGGCGACCTGCTGCCCACCGACAAGCTGCAGGCCATCGAAGACCTGTACGCCCAGGG
CCACCGGGTGGGCATGGTGGGCGACGGCATCAACGACGCCCCGGCCCTGGCCCGTGCCGAAATCGGTTTCGCCATGGCTG
CGGCGGGCACCGACACTGCCATCGAGACCGCGGATGTGGCCCTGATGGACGATGATCTGCGCAAGATCCCGGCGTTTATC
CGCCTGTCGCGCCAGACCTCGGTGGTGCTCAAGCAAAATATCGTCCTGGCCCTGGTCATCAAGGCGATCTTTCTTGGGGT
AACCTTTGCCGGGCTTGCCACCATGTGGATGGCAGTATTCGCCGACATGGGCGTGAGCCTGTTGGTGGTGTTCAACGGTC
TGCGCCTGCTGCGCAAATAA

Protein sequence :
MSDSLHSPRKTADQQPQADHDHDHDHDHDHSHGHSHSHAHAGKLQPVKAHDHAAHGASCCGSAGLAPSRVTLSETPTAGA
RLSSFRIEAMDCPTEQTLIQNKLGKLAGVQALEFNLMNRVLGVTHDLPSTAPIIEAVKSLGMHAEPLEQEGDQAAPAAAP
VKKPWWPLALSGIGALAAEVIHFTNAAPDWVIALVALVSILSGGLTTYKKGWIALKNRNLNINALMSIAVTGAVLIGQWP
EAAMVMFLFTVAELIEARSLDRARNAISGLMQMTPERVTVQQADGNWSEQEAKNVELGALVRVRPGERIGLDGEVVSGNS
TIDQAPITGESLPVEKTVGDKVFAGTINQAGSLEYRVTAAANNSTLARIIHAVEQAQGARAPTQRFVDSFSKIYTPAVFA
FALAVALIPPLFMGGVWFDWIYRALVLLVVACPCALVISTPVSIVSGLAAAARKGILVKGGVYLEGGYKLDYLALDKTGT
ITHGKPVQTDFLSLDPLAQDTAPALAASLAGRSDHPVSLAIAQAAVDKQLAMHPVDNFEALTGRGVRGEINGELYHLGNH
RLVEELGLCSPALEEKLFALEKQGKTVVLLLDRNGPLALFAVADTVKESSREAIQQLHELGIKTLMLTGDNPHTAQAIAA
QVGIDQARGDLLPTDKLQAIEDLYAQGHRVGMVGDGINDAPALARAEIGFAMAAAGTDTAIETADVALMDDDLRKIPAFI
RLSRQTSVVLKQNIVLALVIKAIFLGVTFAGLATMWMAVFADMGVSLLVVFNGLRLLRK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 68
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 5e-113 46
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 2e-112 46
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 2e-112 46

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA_1 YP_262950.2 cadmium-exporting ATPase BAC0298 Protein 0.0 68
cadA_1 YP_262950.2 cadmium-exporting ATPase BAC0461 Protein 1e-76 42