Gene Information

Name : pyk_2 (PFL_4797)
Accession : YP_261878.1
Strain : Pseudomonas protegens Pf-5
Genome accession: NC_004129
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 5537332 - 5538783 bp
Length : 1452 bp
Strand : +
Note : identified by similarity to SP:P21599; match to protein family HMM PF00224; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGTCCGTTCGTCGTACCAAAATCGTTGCCACCCTTGGCCCGGCCAGTAACTCGCCGGAAGTTCTCGAACAGCTGATTCT
CGCGGGCCTGGATGTTGCCCGTCTGAACTTCTCCCACGGCACTCCGGACGAGCACAAGGCTCGCGCCAAACTGGTCCGCG
ACCTGGCAGCCAAGCACGGCCGCTTCGTGGCCATCCTCGGCGACCTGCAGGGCCCGAAGATCCGCATCGCCAAATTCGCC
AACAAGCGCATCGAGCTGAAGATCGGTGACAAGTTCACCTTCTCCACCAGCCATCCGCTGACCGAAGGCACCCAGGATAT
CGTCGGCATCGACTACCCGGATCTGGTCAAGGACTGCGGCGTGGGCGACGAGCTGCTGCTGGACGACGGCCGCGTGGTGA
TGCGTGTGGAAACCGCCACCGCCACCGAGCTGCATTGCGTGGTGACCATCGGCGGCCCGCTGTCCGACCACAAGGGCATC
AACCGTCGTGGTGGTGGCCTGACCGCTCCGGCCCTGACCGAGAAAGACAAGGCCGACATCAAGCTCGCGGCCGAGATGGA
AGTCGATTACCTGGCCGTGTCCTTCCCACGGGACGCCGCCGACATGGAATACGCGCGCAAGCTGCGTGACGAAGCCGGTG
GTACTGCCTGGCTGGTAGCGAAGATCGAACGCGCGGAAGCGGTGGCCGACGACGAAACCCTCGACGGCCTGATCAAGGCC
AGCGACGCGGTCATGGTTGCCCGTGGCGACCTGGGCGTGGAAATCGGCGACGCCGAGCTGGTGGGCATCCAGAAGAAAAT
CATTCTGCACGCCCGTCGCCACAACAAGGCGGTGATCGTTGCGACCCAGATGATGGAGTCGATGATCCAGAACCCGATGC
CGACCCGCGCCGAAGTGTCCGACGTGGCCAACGCCGTGCTCGACTACACCGATGCGGTGATGCTCTCGGCGGAAAGCGCA
GCCGGTGCCTACCCGCTGGAAGCGGTGCAGGCCATGGCGCGGATCTGCGTCGGTGCGGAAAAGCACCCCACCAGCAAGAC
CTCCAGCCACCGCATGGGCAAGGTGTTCGAAAGCTGCGACCAGAGCATCGCCCTGGCTGCCATGTACACCGCCAACCACT
TCCCGGGCGTGAAGGCGATCATCGCCCTGACCGAAAGTGGCTACACCCCGCTGATCATGTCGCGCATTCGCTCCTCGGTG
CCGATCTACGCCTACTCCCCGCACCGCGAAACCCAGGCTCGCGCAGCCATGTTCCGTGGCGTCTACACCATCCCGTTCGA
TCCGGCTTCCCTGCCGCCCGAGCAGGTCAGCCAGGCAGCGATCGATGAGCTGCTCAAGCGCGGTGTCGTAGAGAAAGGCG
ACTGGGTGATTCTCACCAAGGGCGACAGCTACCACACCACCGGCGGCACCAACGGCATGAAGATCCTGCACGTGGGCGAC
CCGATGGTCTGA

Protein sequence :
MSVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRDLAAKHGRFVAILGDLQGPKIRIAKFA
NKRIELKIGDKFTFSTSHPLTEGTQDIVGIDYPDLVKDCGVGDELLLDDGRVVMRVETATATELHCVVTIGGPLSDHKGI
NRRGGGLTAPALTEKDKADIKLAAEMEVDYLAVSFPRDAADMEYARKLRDEAGGTAWLVAKIERAEAVADDETLDGLIKA
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGAYPLEAVQAMARICVGAEKHPTSKTSSHRMGKVFESCDQSIALAAMYTANHFPGVKAIIALTESGYTPLIMSRIRSSV
PIYAYSPHRETQARAAMFRGVYTIPFDPASLPPEQVSQAAIDELLKRGVVEKGDWVILTKGDSYHTTGGTNGMKILHVGD
PMV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 2e-77 46
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-60 45
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-60 45
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-59 45
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-60 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk_2 YP_261878.1 pyruvate kinase VFG0479 Protein 1e-60 45